Abstract
Membrane bound-luminally oriented hepatic UDP-GT is largely unexpressed in liver intact microsomes, and is fully revealed by pretreatment with membrane perturbing agents (latency). Whether the response of UDP -GT to these agents changes during development is poorly known, besides the few existing studies having been carried out without monitoring of microsomal structural integrity. We examined the response of UDP-GT (naphtol as substrate) to a detergent (CHAPSO) and to the putative physiological cofactor NAG in developing rat liver microsomes. Phospholipid (PL) membrane content, and 2 independent parameters of membrane integrity, i.e. latency of low Km microsomal marker Man6Pase and permeability to EDTA, were also assessed.
RESULTS(*p<0.05:developing vs adults)
CONCLUSION We have shown in rat liver intact microsomes that response of UDP-GT to both NAG and detergent activation undergoes major changes during development.
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Vajro, P., Blanckaert, N. & Thaler, M. DEVELOPMENTAL CHANGES OF LATENCY AND UDP-N-ACETYLGLUCOSA MINE (NAG) EFFECT ON HEPATIC UDP-GLUCURONYLTRANSFERASE (UDP-GT) IN RAT LIVER INTACT MICROSOMES. Pediatr Res 22, 242 (1987). https://doi.org/10.1203/00006450-198708000-00173
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DOI: https://doi.org/10.1203/00006450-198708000-00173