Abstract
In an effort to mimic the in vitro situation in CD, the effect of gliadin peptides III and IV on the biosynthesis of a number of BBM hydrolases was studied. Thus biopsies from patients with CD in remission were labelled with 35S-methionine for 24 hours in the presence or absence of gliadin and α-casein. Sucrase (SI), lactase (LPH), maltase (MGA), aminopeptidase N (APN), di-peptidylpeptidase IV (DPPIV) and angiotensin-converting enzyme (ACE) were immunoprecipitated with monoclonal antibodies and analyzed by SDS-PAGE, fluorography and densitometric scannings. Gliadin inhibitory effect on the synthesis of the hydrolases is expressed as % decrease in labelling intensity versus control. Thus SI synthesis was inhibited by 72%, that of MGA and APN by 85-88%, whereas DPPIV, ACE and LPH were completely absent in gliadin-treated biopsies. α-Casein affected the synthesis of SI, LPH and ACE by about 35%, APN was not affected whereas DPPIV was to about 55 %. These values are significantly lower than their counterparts in gliadin-treated biopsies.
Conclusion: This in vitro system provides therefore a promising approach for studying the mechanisms involved in the induction of mucosal damage at the molecular level.
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Nairn, H., Ambühl, P., Sterchi, E. et al. 39. AN IN VITRO MODEL SYSTEM FOR STUDYING THE BIOSYNTHESIS OF BRUSH BORDER MEMBRANE (BBM) HYDROLASES IN COELIAC DISEASE (CD). Pediatr Res 22, 102 (1987). https://doi.org/10.1203/00006450-198707000-00060
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DOI: https://doi.org/10.1203/00006450-198707000-00060