Abstract
The aim of the study was to develop an in vitro method to measure histamine release from human small bowel mucosal mast cells after inmunologic as well as non-immunologic mast cell activation. The method was developed using pieces of healthy small bowel mucosa when it could be obtained from patients who underwent surgery. The mucosal tissue (150 mgs) was treated with collagenase (90 mins/37°C), which dispersed the cells. The suspension (containing 2-8% mast cells) was washed 3 times and incubated with anti-IgE, Con-A and the Ca-ionophore A 23187. Histamine release was measured fluorometrically after adsorbtion to glass microfibers. In 5 non allergic patients Con-A and A 23187 released histamine (dose dependend) in all patients. Two responded to anti-IgE. The basophils from the same patients responded to Con-A in 3 - A 23187 in 5 and Anti-IgE in 4 patients.
The results show that human basophils are not necessarily activated by the same stimuli as the mucosal mast cells in the same patient. The method is believed to be useful in the diagnosis of gastrointestinal allergy.
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Schiôstz, P., Skov, P. & Mauritzen, K. HISTAMINE RELEASE FROM HUMAN BASOPHILS AND SMALL BOWEL MUCOSAL MAST CELLS. Pediatr Res 20, 696 (1986). https://doi.org/10.1203/00006450-198607000-00064
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DOI: https://doi.org/10.1203/00006450-198607000-00064