Researchers have developed a new serial multiphoton microscopy approach to investigate the motility of podocytes and parietal epithelial cells (PECs) in vivo. Using this technique to visualize glomeruli over several days, Hackl and colleagues showed simultaneous migration of multiple podocytes, PEC-to-podocyte migration, and the presence of nanotubules connecting PECs with podocytes, in mouse models of glomerular injury with fluorescent lineage tags.