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Organelle biogenesis

Where did I come from?

Nature Reviews Molecular Cell Biology volume 6, pages 672–673 (2005)Cite this article

The authors worked in Saccharomyces cerevisiae and created a system that allowed the real-time imaging of peroxisome biogenesis. They focused on peroxin-3 (Pex3), an integral membrane protein that is essential for the biogenesis of these organelles. They made a yeast strain that could be induced to express endogenous levels of fluorescently labelled Pex3 (Pex3–YFP) by putting the gene encoding this construct under the control of a galactose-inducible promoter and exposing cells to galactose for a limited time. In the absence of galactose, Pex3 and peroxisomes were absent from these cells.

Using this strain, Tabak and co-workers created two further strains. The first constitutively expressed a fluorescent marker of the ER, whereas the second constitutively expressed a fluorescent marker of peroxisomes. They then used galactose to induce peroxisome reformation in both strains and monitored Pex3–YFP until peroxisomes had reformed. They saw that when Pex3–YFP first appeared, it colocalized with labelled ER structures. It subsequently moved to mature, labelled peroxisomes through dot-like structures, and these observations were confirmed using a biochemical approach.

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References

ORIGINAL RESEARCH PAPER

  1. Hoepfner, D. et al. Contribution of the endoplasmic reticulum to peroxisome formation. Cell 122, 85–95 (2005)

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FURTHER READING

  1. Schekman, R. Peroxisomes: another branch of the secretory pathway? Cell 122, 1–2 (2005)

    Article  CAS  Google Scholar 

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Authors
  1. Rachel Smallridge
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Smallridge, R. Where did I come from?. Nat Rev Mol Cell Biol 6, 672–673 (2005). https://doi.org/10.1038/nrm1725

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