Generating mouse models that have targeted genetic mutations currently relies on the use of targeting vectors and mutant embryonic stem cells (ESCs). Wefers et al. identified a new, quicker way of making mouse models of disease by microinjecting transcription activator-like effector nucleases (TALENs) and synthetic oligodeoxynucleotides into single-cell embryos. The authors created disease-relevant, correctable mutations in the small GTPase RAB38. This technology enabled heterozygous mutant mice to be available within 18 weeks (ESC-based mutant mice can take a year or longer to produce).
References
Wefers, B. et al. Direct production of mouse disease models by embyro microinjection of TALENs and oligodeoxynucleotides. Proc. Natl Acad. Sci. USA 110, 3782–3787 (2013)
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Charlotte, H. A speedy route to mutant mice. Nat Rev Drug Discov 12, 346 (2013). https://doi.org/10.1038/nrd4020
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DOI: https://doi.org/10.1038/nrd4020
