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In vivo models of proliferative vitreoretinopathy

Nature Protocols volume 2, pages 67–77 (2007)Cite this article

Abstract

We outline current in vitro and in vivo models for experimental proliferative vitreoretinopathy (PVR) and provide a detailed protocol of our standardized in vivo PVR model. PVR is the leading cause of failed surgical procedures for the correction of rhegmatogenous retinal detachment. The pathogenesis of this multifactorial condition is still not completely understood. Experimental models for PVR help us understand the factors that play a role in the pathogenesis of the disease process in a controlled manner and allow for reproducible preclinical assessment of novel therapeutic interventions. We describe a cell injection model in detail that uses homologous retinal pigment epithelial (RPE) cell cultures to induce PVR over a 2–8 week period.

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Figure 1: Rabbit eye with the cornea and lens removed.
Figure 2: Rabbit eye is prepared for surgery as described.
Figure 3: RPE cell injection into the eye.
Figure 4: Classification of experimental PVR in a rabbit eye.

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Acknowledgements

The authors would like to thank Ernesto Barron for assistance with the figures and Susan Clarke for editorial assistance. This work was supported by National Institutes of Health grant EY02061 and a National Eye Institute core grant to the Doheny Eye Institute (EY03040).

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Authors and Affiliations

  1. Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, 90033, CA, USA

    Rajat N Agrawal, Shikun He, Christine Spee, Stephen J Ryan & David R Hinton

  2. Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, 90033, CA, USA

    Shikun He & David R Hinton

  3. Doheny Eye Institute, Keck School of Medicine of the University of Southern California, Los Angeles, 90033, CA, USA

    Rajat N Agrawal, Shikun He, Christine Spee, Stephen J Ryan & David R Hinton

  4. Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, V5Z 3N9, BC, Canada

    Jing Z Cui

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Correspondence to David R Hinton.

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Agrawal, R., He, S., Spee, C. et al. In vivo models of proliferative vitreoretinopathy. Nat Protoc 2, 67–77 (2007). https://doi.org/10.1038/nprot.2007.4

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