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Ultrahigh density microarrays of solid samples

Nature Methods volume 2, pages 511–513 (2005)Cite this article

Abstract

We present a sectioning and bonding technology to make ultrahigh density microarrays of solid samples, cutting edge matrix assembly (CEMA). Maximized array density is achieved by a scaffold-free, self-supporting construction with rectangular array features that are incrementally scalable. This platform technology facilitates arrays of >10,000 tissue features on a standard glass slide, inclusion of unique sample identifiers for improved manual or automated tracking, and oriented arraying of stratified or polarized samples.

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Figure 1: Ultrahigh density microarraying of solid samples using CEMA.
Figure 2: Utility of CEMA arrays for broad range of substrates.

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Acknowledgements

Animal studies were approved by Georgetown University Animal Care and Use Committee. Supported by US National Institutes of Health grants R01-DK52013 and R01-CA101841 (to H.R.) and T32-CA009686-08 (to M.J.L.). We thank Lombardi Comprehensive Cancer Center shared resources of Histopathology, Microscopy and Imaging, and Tissue Culture (supported in part by NIH 1P30-CA-51008), and K. Catlin-LeBaron for help with graphic design.

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Authors and Affiliations

  1. Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, NRB E504, 3970 Reservoir Rd, NW, DC 20057-1469, Washington, USA

    Matthew J LeBaron, Heidi R Crismon, Fransiscus E Utama, Lynn M Neilson, Ahmed S Sultan, Kevin J Johnson, Eva C Andersson & Hallgeir Rui

Authors
  1. Matthew J LeBaron
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  2. Heidi R Crismon
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  3. Fransiscus E Utama
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  4. Lynn M Neilson
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  5. Ahmed S Sultan
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  6. Kevin J Johnson
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  7. Eva C Andersson
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  8. Hallgeir Rui
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Corresponding author

Correspondence to Hallgeir Rui.

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Competing interests

Intellectual property arising from this work belongs to Georgetown University (GU). A patent application, which covers the work described in this manuscript, naming H.R and M.J.L. as inventors has been filed and is managed by GU Office of Technology Licensing according to institutional guidelines.

Supplementary information

Supplementary Fig. 1

Ultrahigh density microarraying of solid samples using CEMA. (PDF 761 kb)

Supplementary Fig. 2

CEMA arrays of stratified tissues and 3D cell culture samples. (PDF 1453 kb)

Supplementary Fig. 3

Frozen tissue array of skeletal muscle. (PDF 496 kb)

Supplementary Methods (PDF 103 kb)

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LeBaron, M., Crismon, H., Utama, F. et al. Ultrahigh density microarrays of solid samples. Nat Methods 2, 511–513 (2005). https://doi.org/10.1038/nmeth772

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