Jones, A.M. et al. Science 344, 711–716 (2014).

A large subset of the interactome—the set of all physical interactions between proteins—remains unknown for most species. Identifying potential interactors of membrane proteins is particularly challenging. Jones et al. now use the established yeast split-ubiquitin assay to screen for the interactions of more than 3,000 membrane proteins from Arabidopsis, between one another and between the membrane proteins and over 1,000 cytosolic signaling factors. The 30,000 interactions identified, which constitute less than 1% of all tested pairs, were further validated in a secondary screen, yielding a set of about 12,000 interactions termed the Membrane-linked Interactome Database version 1 (MIND1). This data set contains many previously unknown interactions and should prove a useful resource for the study of membrane-protein function in Arabidopsis.