Wylie et al. describe a method to pattern growth factors in a three-dimensional hydrogel in a controllable manner. They introduced reactive thiol sites in the hydrogel with selective two-photon uncaging of coumarin-caged thiols; these sites serve as anchors for proteins such as streptavidin or barnase. Growth factors can then be immobilized at these sites by fusing them to the cognate binding partners of streptavidin, biotin, or barnase, barstar. Growth factors introduced in this manner guided cell migration in the hydrogel.
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Growth-factor immobilization in hydrogels. Nat Methods 8, 791 (2011). https://doi.org/10.1038/nmeth.1726
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DOI: https://doi.org/10.1038/nmeth.1726
