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Reply to 'VLA-5 and transendothelial migration'

Nature Medicine volume 8, pages 765–766 (2002)Cite this article

Zou and Curiel reply

We thank Drs. Sanchez-Mateos, de la Rosa and Longo for their thorough analysis of our work1. As they point out, VCAM-1 is not a ligand for VLA-5, and therefore experiments with anti-VCAM-1 should not be used to draw conclusions regarding the potential role for VLA-5 in cell migration. Our work demonstrated that tumor-associated preDC2s expressed CD49d (VLA-4) equivalently to blood preDC2s, whereas CD49e (VLA-5) was expressed to a significantly greater degree. Further, tumor-derived SDF-1, which we implicated in tumor immunopathology, increased VLA-5 expression in blood preDC2s. Thus, our data implicated VLA-5 as a potential mediator of preDC2 adhesion/transmigration. The correct experiment, as pointed out by Sanchez-Mateos et al., is to use anti-VLA-5 on preDC2s, and evaluate the effect on cell trafficking, which we have now done.

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  1. Section of Hematology and Medical Oncology Tulane Medical School, New Orleans, Louisiana, USA

    Weiping Zou & Tyler Curiel

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  1. Weiping Zou
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Zou, W., Curiel, T. Reply to 'VLA-5 and transendothelial migration'. Nat Med 8, 765–766 (2002). https://doi.org/10.1038/nm0802-765b

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