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Functional evidence for a single endodermal origin for the thymic epithelium

Nature Immunology volume 5, pages 546553 (2004) | Download Citation

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Abstract

T cell development depends critically on several distinct thymic epithelial cell types that are organized into two main compartments: cortex and medulla. The prevailing hypothesis suggests that these derive from ectoderm and endoderm, respectively. Here we show that lineage analysis provides no evidence for an ectodermal contribution to the thymic rudiment. We further demonstrate, via ectopic transplantation, that isolated pharyngeal endoderm can generate a functional thymus containing organized cortical and medullary regions and that this capacity is not potentiated by the presence of pharyngeal ectoderm. These data establish that the cortical and medullary thymic epithelial compartments derive from a single germ layer, the endoderm, thus refuting the 'dual-origin' model of thymic epithelial ontogeny.

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Acknowledgements

We thank A. Medvinsky for critical discussion, C.L. Bennett for assistance with flow cytometry and Biomed Unit staff for animal care. Supported by the Medical Research Council, UK (C.C.B., J.G., J.S. and V.A.W.), Leukaemia Research Fund, UK (C.C.B., N.B. and A.F.), National Institutes of Health-National Institute of Child Health and Human Development (HD035920 to N.R.M.) and Wellcome Trust (C.C.B. and N.R.M.).

Author information

Author notes

    • Julie Gordon

    Present address: Department of Genetics, Life Sciences Building, University of Georgia, Athens, Georgia 30602, USA.

Affiliations

  1. Institute for Stem Cell Research, The University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JQ, UK.

    • Julie Gordon
    • , Valerie A Wilson
    • , Natalie F Blair
    • , Julie Sheridan
    • , Alison Farley
    •  & C Clare Blackburn
  2. Division of Biomedical and Clinical Laboratory Science, The University of Edinburgh, George Square, Edinburgh EH8 9XD, UK.

    • Linda Wilson
  3. Department of Genetics, Life Sciences Building, University of Georgia, Athens, Georgia 30602, USA.

    • Nancy R Manley

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The authors declare no competing financial interests.

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Correspondence to C Clare Blackburn.

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DOI

https://doi.org/10.1038/ni1064

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