Abstract
The subdivision of total genomic human yeast artificial chromosome (YAC) libraries into specific chromosome clone collections will greatly facilitate the construction of an integrated genetic, physical and transcriptional map of the genome. We report the isolation of 388 YAC clones from a human library with an average insert size of 620 kilobases (kb) by the hybridization of a composite chromosome 21 probe to a high–density array of YAC clones. Roughly half of these clones hybridize to chromosome 21 by fluorescence in situ hybridization. These clones represent a twofold coverage of the chromosome. The technique offers the potential of sub–dividing whole genomic YAC libraries into their chromosomal elements to produce high–resolution tools for genome mapping.
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References
Burke, D.T. et al. Science 236, 806–812 (1987).
Larin, Z. et al. Proc. natn. Acad. Sci. U.S.A. 88, 4123–4127 (1991).
Green, E.D. & Olson, M.V. Proc. natn. Acad. Sci. U.S.A. 87, 1213–1217 (1990).
Olson, M. et al. Science 245, 1434–1435 (1989).
Monaco, A.P. et al. Nucl. Acids Res. 19, 3315–3318 (1991).
Nizetic, D. et al. Proc. natn. Acad. Sci. U.S.A. 88, 3233–3237 (1991).
Ross, M.T. et al. in Techniques for the Analysis of Complex Genomes (ed. Anand, R.) 137–153 (Academic Press, London, 1992).
Maier, E. et al. Nature Genet. 1, 273–277 (1992).
McCormick, M.K. et al. Proc. natn. Acad. Sci. U.S.A. 86, 9991–9995 (1989).
Abidi, F.E. et al. Genomics 7, 363–376 (1990).
Schlessinger, D. et al. Genomics 11, 783–793 (1991).
Albertsen, H.M. et al. Proc. natn. Acad. Sci. U.S.A. 87, 4256–4260 (1990).
Collins, C. et al. Genomics 11, 997–1006 (1991).
Van Dilla, M.A. et al. Biotechnology 4, 537–552 (1986).
Van Dilla, M. et al. in Flow cytogenetics (ed. Gray, J.) 257–274 (Academic Press, New York, 1989).
Baldini, A. et al. Genomics (in the press).
Henderson, A. et al. Proc. natn. Acad. Sci. U.S.A. 69, 3394–3398 (1972).
Kurnit, D. et al. Cytogenet Cell Genet. 38, 99–105 (1984).
Kurnit, D. et al. Cytogenet. Cell Genet. 43, 109–116 (1986).
Riley, J. et al. Nucl. Acids Res. 18, 2887–2890 (1990).
Millington-Ward, A. et al. Cytogenet Cell Genet. 40, 699 (1985).
Tantravahi, U. et al. Hum. Genet. 79, 196–202 (1988).
Burmeister, M. et al. Genomics 9, 19–30 (1991).
Chumakov, I.M. et al. Nature Genet. 1, 222–225 (1992).
Lehrach, H. et al. in Genome Analysis: Genetic and Physical Mapping Vol 1 (eds Davies, K.E. & Tilghman, S.M.) 39–81 (Cold Spring Harbor Laboratory Press, 1990).
Baxendale, S. et al. Nucl. Acids Res. 19, 6651 (1991).
Larin, Z. & Lehrach, H., Genet. Res. (Camb.) 56, 203–208 (1990).
Sealey, P.G. et al. Nucl. Acids Res. 13, 1905–1922 (1985).
Lichter, P. et al Science 247, 64–69 (1990).
Ried, T. et al. Proc. natn. Acad. Sci. U.S.A. 89, 1388–1392 (1992).
Baldini, A. & Ward, D.C. Genomics 9, 770–774 (1991).
Van Camp, G. et al. Hum Genet. 87, 649–653 (1991).
Patterson, D. Cytogenet. Cell Genet. 57, 167–174 (1991).
Korenberg, J.R. et al. Cytogenet. Cell Genet. 40, 673 (1985).
Cox, D.R. & Shimizu, N. Cytogenet. Cell Genet. 58, 800–826 (1991).
Van Hul, W. et al. Cytogenet. Cell Genet. 58, 2041 (1991).
Gardiner, K. et al. EMBO J. 9, 25–34 (1990).
Cox, D.R. et al. Science 250, 245–250 (1990).
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Ross, M., Nižetić, D., Nguyen, C. et al. Selection of a human chromosome 21 enriched YAC sub–library using a chromosome–specific composite probe. Nat Genet 1, 284–290 (1992). https://doi.org/10.1038/ng0792-284
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DOI: https://doi.org/10.1038/ng0792-284