Abstract
A whole-genome radiation hybrid (RH) panel was used to construct a high-resolution map of the rat genome based on microsatellite and gene markers. These include 3,019 new microsatellite markers described here for the first time and 1,714 microsatellite markers with known genetic locations, allowing comparison and integration of maps from different sources. A robust RH framework map containing 1,030 positions ordered with odds of at least 1,000:1 has been defined as a tool for mapping these markers, and for future RH mapping in the rat. More than 500 genes which have been mapped in mouse and/or human were localized with respect to the rat RH framework, allowing the construction of detailed rat-mouse and rat-human comparative maps and illustrating the power of the RH approach for comparative mapping.
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Acknowledgements
We thank the staff of Otsuka GEN Research Institute, especially Y. Kuga, A. Takaichi, F. Aki, Y. Fujii, N. Haraguchi, K. Higashi, S. Kato, K. Nakagawa, Y. Nakahara, A. Sano, T. Suto, K. Yamada and T. Wakamatsu for their technical assistance; T. Matise for help with MultiMap; P. Rodriguez-Tomé and P. Lijnzaad for help with RHdb submissions; and R.E. White for his generous help. Work in Oxford was supported by the Wellcome Trust. G.M.L. is a Wellcome Trust Principal Research Fellow. Work in both Oxford and Cambridge was partly supported by EC Biotechnology Grant BIO4-CT96-0372. Work in the laboratory of M.R.J. also used tools developed under EC grant BMH4-CT95-1565 and Wellcome Trust grant 045148/B/95.
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Watanabe, T., Bihoreau, MT., McCarthy, L. et al. A radiation hybrid map of the rat genome containing 5,255 markers. Nat Genet 22, 27–36 (1999). https://doi.org/10.1038/8737
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DOI: https://doi.org/10.1038/8737
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