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Simple purification of human chromosomes to homogeneity using muntjac hybrid cells

Nature Genetics volume 7, pages 29–33 (1994)Cite this article

Abstract

Chromosome sorting from hybrid cells offers enormous advantages for gene mapping and cloning, but purification of most chromosomes has been largely hindered by their similarity in size to other chromosomes. We have developed a novel cell line and strategy that allows simple, mass purification of mammalian chromosomes, permitting significant target genome enrichment. This strategy takes advantage of the small number of giant chromosomes (1,2,X) of the female Indian muntjac, a barking deer, avoiding the problem of size similarity. We introduced human chromosomes into a cell line derived from a muntjac and purified them to homogeneity using a relatively simple technique.This strategy should facilitate the isolation of chromosomes from species other than human for which hybrid cells are not available currently.

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Author notes

  1. Andrew P. Feinberg

    Present address: Departments of Medicine and Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, Maryland, 21205, USA

Authors and Affiliations

  1. Howard Hughes Medical Institute and Departments of Internal Medicine and Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan, 48109, USA

    Jae-Yong Lee, Minoru Koi & Andrew P. Feinberg

  2. Department of Microbiology and Molecular Genetics, University of California Irvine, College of Medicine, Irvine, California, 92717, USA

    Eric J. Stanbridge

  3. Department of Molecular and Cell Genetics, School of Life Sciences, Tottori University, Nishimachi 86, Yonago-city, Tottori-ken, 683, Japan

    Mitsuo Oshimura

  4. Center for Reproduction of Endangered Species, Zoological Society of San Diego, San Diego, California, 92112, USA

    Arlene T. Kumamoto

Authors
  1. Jae-Yong Lee
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  2. Minoru Koi
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  3. Eric J. Stanbridge
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  4. Mitsuo Oshimura
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  5. Arlene T. Kumamoto
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  6. Andrew P. Feinberg
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Lee, JY., Koi, M., Stanbridge, E. et al. Simple purification of human chromosomes to homogeneity using muntjac hybrid cells. Nat Genet 7, 29–33 (1994). https://doi.org/10.1038/ng0594-29

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  • DOI: https://doi.org/10.1038/ng0594-29

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