Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

RNA Synthesis Startpoints in Bacteriophage λ: Are the Promoter and Operator Transcribed?

Nature New Biology volume 237, pages 227–232 (1972)Cite this article

Abstract

Hybridization and fingerprint analysis of in vitro synthesized λ RNA shows that four chains are initiated at sites corresponding to those seen in vivo, and that each molecule starts with a specific sequence. In one case examined, the major leftward operon, the promoter and operator and not transcribed into RNA.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).

    Article  CAS  Google Scholar 

  2. Szybalski, W., Bøvre, K., Flandt, M., Hayes, M., Hradecna, Z., Kumar, S., Lozeron, H. A., Nijkamp, H. J. J., and Stevens, W. F., Cold Spring Harbor Symp. Quant. Biol., 25, 341 (1970).

    Article  Google Scholar 

  3. Blattner, F. R., Dahlberg, J. E., Boettiger, J. K., Fiandt, M., and Szybalski, W., Nature New Biology, 237, 232 (1972).

    Article  CAS  Google Scholar 

  4. Roberts, J. W., Nature, 224, 1168 (1969).

    Article  CAS  Google Scholar 

  5. Davis, R. W., and Hyman, R. W., Cold Spring Harbor Symp. Quant. Biol., 35, 269 (1970).

    Article  CAS  Google Scholar 

  6. Szybalski, W., Boslash;vre, K., Flandt, M., Guha, A., Hradecna, Z., Kumar, S., Lozeron, H. A., Maher, V. M., Nijkamp, K. J. J., Summers, W. C., and Taylor, K., J. Cell. Physiol., 74 (Suppl. 1), 33 (1969).

  7. Champoux, J. J., Cold Spring Harbor Symp. Quant. Biol., 35, 319 (1970).

    Article  CAS  Google Scholar 

  8. Hayes, S. J., Fed. Proc., 31, 444, Abstr. (1972).

    Google Scholar 

  9. Reichart, L., and Kaiser, A. D., Proc. US Nat. Acad. Sci., 68, 2185 (1971).

    Article  Google Scholar 

  10. Herskowitz, I., and Singer, E., Cold Spring Harbor Symp. Quant. Biol., 35, 355 (1970).

    Article  CAS  Google Scholar 

  11. Taylor, K., Hradecna, Z., and Szybalski, W., Proc. US Nat. Acad. Sci., 57, 1618 (1967).

    Article  CAS  Google Scholar 

  12. Szybalski, W., Kubinski, H., and Sheldrick, P., Cold Spring Harbor Symp. Quant. Biol., 31, 123 (1966).

    Article  CAS  Google Scholar 

  13. Champoux, J. J., thesis, Stanford Univ. (1969).

  14. Takanami, M., Okamoto, T., and Sugiura, M., Cold Spring Harbor Symp. Quant. Biol., 35, 179 (1970).

    Article  CAS  Google Scholar 

  15. Sanger, F., Brownlee, G. G., and Barrell, B. G., J. Mol. Biol., 13, 373 (1965).

    Article  CAS  Google Scholar 

  16. Billeter, M. A., Dahlberg, J. E., Goodman, H. M., Hindley, J., and Weissmann, C., Cold Spring Harbor Symp. Quant. Biol., 34, 635 (1969).

    Article  CAS  Google Scholar 

  17. Lebowitz, P., Wiesman, S. M., and Radding, C. M., J. Biol. Chem., 246, 5120 (1971).

    CAS  PubMed  Google Scholar 

  18. Marcaud, L., Portier, M. M., Kourilsky, P., Barrell, B. G., and Gros, F., J. Mol. Biol., 57, 247 (1971).

    Article  CAS  Google Scholar 

  19. Lozeron, H. A., Funderburgh, M. L., and Szybalski, W., Fed. Proc., 30, 1315, Abstr. (1971).

    Google Scholar 

  20. Lozeron, H. A., Funderburgh, M. L., Dahlberg, J. E., Stark, B. P., and Szybalski, W., Abstracts Ann. Meet. Amer. Soc. Microbiol., 237 (1972).

  21. Blattner, F. R., Boettiger, J. K., Fiandt, M., Lozeron, H. A., Funderburgh, M. L., Dahlberg, J. E., and Szybalski, W., Fed. Proc., 31, 472, Abstr. (1972).

    Google Scholar 

  22. Gottesman, M. E., and Weisberg, R. A., in The Bacteriophage Lambda (edit, by Hershey, A. D.), 113 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  23. Jacob, F., and Wollman, E. L., Ann. Inst. Pasteur, 87, 653 (1954).

    CAS  Google Scholar 

  24. Ptashne, M., in The Bacteriophage Lambda (edit, by Hershey, A. D.), 221 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  25. Nijkamp, H. J. J., Bøvre, K., and Szybalski, W., J. Mol. Biol., 54, 599 (1970).

    Article  CAS  Google Scholar 

  26. Kaiser, A. D., and Jacob, F., Virology, 4, 504 (1957).

    Article  Google Scholar 

  27. Kumar, S., and Szybalski, W., Virology, 47, 665 (1970).

    Article  Google Scholar 

  28. Chadwick, P., Pirrotta, V., Steinberg, R., Hopkins, N., and Ptashne, M., Cold Spring Harbor Symp. Quant. Biol., 35, 283 (1970).

    Article  CAS  Google Scholar 

  29. Steinberg, R. A., and Ptashne, M., Nature New Biology, 230, 76 (1971).

    Article  CAS  Google Scholar 

  30. Simon, M. N., Davis, R. W., and Davidson, N., in The Bacteriophage Lambda (edit, by Hershey, A. D.), 313 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  31. Westmoreland, B. C., Szybalski, W., and Ris, H., Science, 163, 1343 (1969).

    Article  CAS  Google Scholar 

  32. Pirrotta, V., and Ptashne, M., Nature, 111, 541 (1969).

    Article  Google Scholar 

  33. Pereira da Silva, L., and Jacob, F., Ann. Inst. Pasteur, 115, 145 (1968).

    Google Scholar 

  34. Chamberlin, M., and Berg, P., Proc. US Nat. Acad. Sci., 48, 81 (1962).

    Article  CAS  Google Scholar 

  35. Burgess, R. R., Travers, A. A., Dunn, J. J., and Bautz, E. K. F., Nature, 111, 43 (1969).

    Article  Google Scholar 

  36. Symons, R. H., Biochim. Biophys. Acta, 190, 548 (1969).

    Article  CAS  Google Scholar 

  37. Nygaard, A. P., and Hall, B. D., J. Mol. Biol., 9, 125 (1964).

    Article  CAS  Google Scholar 

  38. Bøvre, K., Lozeron, H. A., and Szybalski, W., in Methods in Virology (edit, by Maramorosch, K., and Koprowski, H.), 5, 271 (Academic Press, New York, 1971).

    Google Scholar 

  39. Fiandt, M., Hradecna, Z., Lozeron, H. A., and Szybalski, W., in The Bacteriophage Lambda (edit, by Hershey, A. D.), 329 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  40. Court, D., and Sato, K., Virology, 39, 348 (1969).

    Article  CAS  Google Scholar 

  41. Kayajanian, G., Virology, 41, 170 (1970).

    Article  CAS  Google Scholar 

  42. Kayajanian, G., Virology, 36, 30 (1968).

    Article  CAS  Google Scholar 

  43. Guha, A., Saturen, Y., and Szybalski, W., J. Mol. Biol., 56, 53 (1971).

    Article  CAS  Google Scholar 

  44. Stevens, W. F., Adhya, S., and Szybalski, W., in The Bacteriophage Lambda (edit, by Hershey, A. D.), 515 (Cold Spring Harbor Laboratory, New York, 1971).

    Google Scholar 

  45. Ghysen, A., and Pirono, M., J. Mol. Biol., (in the press).

  46. Blattner, F. R., Boettiger, J. K., and Dahlberg, J. E., Fed. Proc., 30, 1315, Abstr. (1971).

    Google Scholar 

  47. Schös, M., and Inman, R. B., J. Mol. Biol., 55, 316 (1970).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin, 53706

    FREDERICK R. BLATTNER

  2. Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin, 53706

    JAMES E. DAHLBERG

Authors
  1. FREDERICK R. BLATTNER
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. JAMES E. DAHLBERG
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

BLATTNER, F., DAHLBERG, J. RNA Synthesis Startpoints in Bacteriophage λ: Are the Promoter and Operator Transcribed?. Nature New Biology 237, 227–232 (1972). https://doi.org/10.1038/newbio237227a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio237227a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing