Article | Open

Biological iron-sulfur storage in a thioferrate-protein nanoparticle

  • Nature Communications 8, Article number: 16110 (2017)
  • doi:10.1038/ncomms16110
  • Download Citation
Published online:


Iron–sulfur clusters are ubiquitous in biology and function in electron transfer and catalysis. They are assembled from iron and cysteine sulfur on protein scaffolds. Iron is typically stored as iron oxyhydroxide, ferrihydrite, encapsulated in 12 nm shells of ferritin, which buffers cellular iron availability. Here we have characterized IssA, a protein that stores iron and sulfur as thioferrate, an inorganic anionic polymer previously unknown in biology. IssA forms nanoparticles reaching 300 nm in diameter and is the largest natural metalloprotein complex known. It is a member of a widely distributed protein family that includes nitrogenase maturation factors, NifB and NifX. IssA nanoparticles are visible by electron microscopy as electron-dense bodies in the cytoplasm. Purified nanoparticles appear to be generated from 20 nm units containing 6,400 Fe atoms and 170 IssA monomers. In support of roles in both iron–sulfur storage and cluster biosynthesis, IssA reconstitutes the [4Fe-4S] cluster in ferredoxin in vitro.


Iron is an essential nutrient for almost all known organisms. It functions as a protein cofactor in fundamental pathways including respiration, photosynthesis and the biogeochemical cycling of sulfur and nitrogen. There are two major types of iron-containing protein cofactors, haemes and iron–sulfur clusters. The most common iron–sulfur cluster is the cubane-type [4Fe-4S] cluster, which is involved in electron transfer, catalysis, DNA repair and small molecule sensing1. In spite of their high sensitivity to degradation by oxygen and reactive oxygen species, [4Fe-4S] clusters are ubiquitous in biology. More complex iron–sulfur-containing cofactors containing modified [4Fe-4S] clusters catalyse more chemically challenging reactions in nitrogenase, carbon monoxide dehydrogenase, acetyl-coA synthase and hydrogenase1.

Iron–sulfur cluster biosynthesis is carried out by two main systems in microorganisms. Bacteria (and mitochondria) typically use the ISC system while archaea and some bacteria (and some plastids) use the SUF system2. The ISC and SUF systems have similar mechanisms wherein an iron donor and a cysteine desulfurase provide Fe and S to a scaffold protein, and the metastable scaffold-bound cluster is rapidly delivered to a carrier protein3. Due to the complex chemistry and energetic cost involved (ATP is used in initiating cluster release or recruiting Fe and cysteine must be regenerated), it is efficient for a cell to repair iron-sulfur clusters that become damaged by reactive oxygen or nitrogen species, and several repair systems have been proposed4,5. In particular, many [4Fe-4S]2+ clusters will lose Fe reversibly upon oxidation, to form cubane-type [3Fe-4S]+ clusters or cysteine persulfide-ligated [2Fe-2S]2+ clusters. Repair of such degraded forms is efficient because they can be restored simply by reduction and the addition of ferrous iron without the need for the full biosynthetic machinery.

While the identity or need for a specific Fe donor for iron-sulfur cluster biosynthesis is still under debate6, Fe import and storage systems allow cells to avoid the toxicity of free ferrous iron in the presence of O2 while maintaining sufficient cellular iron in spite of the insolubility of free ferric iron at neutral pH7,8. In many organisms, Fe is stored in ferritin, which assembles into a 24-mer hollow sphere. Ferritin catalyses the assembly (and release) of a ferrihydrite-type ferric oxy-hydroxide (FeOOH) from Fe2+ and O2 in the interior of the sphere using catalytic iron sites, but the mechanism of Fe release from the mineral core is still largely unresolved. Although ferritin homologues exist throughout the three domains of life, including anaerobes, an anaerobic oxidant that facilitates oxidation of Fe2+ has yet to be identified, and the physiological role of ferritin in many organisms is not clear8. The exact composition of the iron oxide component of ferritin also varies among different species, as does its crystallinity8. Abiotic ferrihydrite is disordered or nanocrystalline and porous9. Thus this material lends itself to a highly dense form of iron storage that maintains accessibility of the iron due to the high specific area (300 m2 g−1) and metastability (relative to other minerals)9.

To investigate the process of iron and sulfur storage and their incorporation into iron-sulfur clusters in an anaerobic micro-organism that cannot use oxygen to oxidize ferrous iron, we examined the archaeon Pyrococcus furiosus, which grows optimally near 100 °C in hydrothermal marine vents10. This strict anaerobe grows in the presence of elemental sulfur (S0) and uses it as an (insoluble) electron acceptor to generate (soluble) hydrogen sulfide10. It has been shown that at least some Archaea use extracellular sulfide directly, rather than cysteine, for iron-sulfur cluster biosynthesis11. P. furiosus does contain a homologue of the SufS cysteine desulfurase1,2, but its natural hydrothermal vent environment is typically rich in sulfide and this could be directly incorporated into Fe–S clusters, which would be much more efficient than cysteine degradation to produce sulfide. In support of this idea, transcriptional analysis comparing P. furiosus grown with and without S0 revealed upregulation in the expression of numerous genes during hydrogen sulfide production, including those involved in iron and iron-sulfur cluster metabolism12. However, the most highly upregulated of these genes (PF2025) encodes a conserved hypothetical protein that at the time was termed sulfur-induced protein A, or SipA (ref. 13). Since expression of sipA is only upregulated by sulfide in the presence of sufficient iron14, this prompted us to investigate whether this protein acts in iron-sulfur cluster metabolism with direct sulfide incorporation. Based on the results presented herein, we rename SipA to the more specific iron–sulfur storage protein A, or IssA.

In this study, we present characterization of IssA. Transmission electron microscopy (TEM) of P. furiosus cells expressing IssA shows naturally electron-dense particles that co-locate with IssA immunolabelling. TEM of purified IssA reveals particles up to 300 nm, which appear to be comprised of 20 nm spheres. X-ray absorption spectroscopy (XAS) strongly supports a thioferrate-type linear (FeS2)n structure of iron and sulfur, and the EPR of IssA is in accord with this assignment. Finally, we show that IssA is capable of assembling a [4Fe-4S] cluster on P. furiosus ferredoxin (Fd), an abundant electron carrier in this organism, in the presence of the small molecule thiol, dithiothreitol (DTT). These properties of IssA together with the conditions under which it is expressed13,14 lead us to conclude that P. furiosus stores excess Fe and S, when they are both highly abundant, in IssA-bound thioferrate, an iron–sulfur structure not previously known in biology. The stored thioferrate can subsequently be mobilized for assembly of [4Fe-4S] clusters, which are widely used in P. furiosus. Phylogenetic analyses suggest that homologues of IssA in many archaea, and possibly bacteria as well, may also store iron as thioferrate.


Expression of IssA nanoparticles is sulfur-responsive

When S0 was added to a growing P. furiosus culture, IssA could be detected in cells by TEM and immuno-gold labelling after 20 min (Fig. 1a). IssA concentration increased over subsequent hours (Supplementary Fig. 1) as well as the area of the cytoplasm occupied by IssA (Fig. 1a), eventually reaching 10–30% of the sectioned cellular area (Fig. 1). IssA is visible not only by immunolabelling but also as electron-dense blotches that co-locate with immunolabelling of IssA both spatially and temporally (Fig. 1). These observations indicate that this protein forms large (≥50 nm) aggregates in vivo after 1 h of IssA expression. Energy dispersive X-ray analysis of the TEM-visualized electron-dense blotches indicated that IssA is associated with iron and sulfur in vivo (Supplementary Fig. 2). In addition, proteomic analysis of IssA immunoprecipitated from P. furiosus cell extract showed that no other protein is associated with the purified IssA nanoparticle in vitro (Supplementary Table 1), suggesting homomeric IssA nanoparticles exist in vivo.

Scheme 1: Electron micrographs showing IssA assemblies in vivo.
Figure 1

(a) The area of IssA localization (immunolabelled with gold nanoparticles) increases over time after addition of S0 to a growing culture of P. furiosus (scale bars are 200 nm). (b) Cells grown using a S0 electron acceptor exhibit native electron-dense regions that overlap with immunolabelled IssA (scale bar is 200 nm).

IssA binds iron and sulfur

IssA was purified from cell extracts of S0-grown P. furiosus based on its massive size and high-density relative to other cellular components. Even though the amino acid sequence showed no indication of membrane-association, when cytoplasmic and membrane-associated proteins were separated by ultracentrifugation, IssA was the major protein in the sedimented pellet, as determined by SDS-gel electrophoresis (Supplementary Fig. 3). The membrane-bound proteins in the pellet were dissolved by treatment with detergent (sodium dodecyl sulfate, 1%), however, IssA was not solubilized by this treatment. Density equilibrium centrifugation of the detergent-insoluble material resulted in a black band in which IssA is the only protein present (Supplementary Fig. 3). Metal analysis by inductively coupled plasma mass spectrometry (53 elements) of purified IssA showed that it contains approximately 38 iron atoms and 1 zinc atom per 19 kDa IssA monomer (Supplementary Table 2). Colorimetric assays indicated that IssA contains 38 acid-labile sulfide ions and 17 sulfane sulfur atoms per protein monomer. The interpretation of the sulfur measurements is discussed further below.

Characterization of IssA nanoparticles

Sedimentation of IssA after ultracentrifugation was consistent with the observation of large particles via TEM. This was investigated further using size exclusion chromatography. Most (90%) of the protein was too large to enter the chromatography column and was retained on the pre-column filter (diameter 1 μm). IssA that entered and eluted from the chromatography column did so in a peak at the exclusion limit (40 MDa) or just after the exclusion limit (100 MDa dextran or 400 nm spheres) from Superose 6 and Sephacryl S-1000 SF columns, respectively (Supplementary Fig. 4). Dynamic light scattering analysis was attempted to obtain more precise sizing information. However, reproducible results could not be obtained from IssA. Centrifugation to minimize dynamic light scattering interference by small air bubbles led to sedimentation of IssA, overall signal was low due to the high extinction coefficient of IssA samples (Gregory L. Hura, personal communication), which appear black, and IssA particles are not uniform in size, as was observed on electron micrographs.

Negative stain TEM of the purified protein showed IssA assemblages with dimensions ranging from 20 to 300 nm (Fig. 2a). Incubation of IssA with 3 M guanidinium chloride, 0.5 mM EDTA and 25 mM DTT, however, yields a more monodisperse sample comprised of roughly spherical particles 16–22 nm in diameter (Fig. 2b). Thus it appears that the 19 kDa IssA monomer forms modular nanostructures based on a 20 nm packing unit, containing 6,400 Fe atoms and 170 IssA monomers. A protein complex of this size of average density would have a mass of 3.5 MDa.

Scheme 2: Electron micrographs showing IssA assemblies in vitro.
Figure 2

(a) IssA is purified as nanoparticles of 20–300 nm as shown by negative stain TEM (scale bar is 50 nm). (b) Magnified region of a to match scale of c. (c) Treatment of IssA with guanidinium, EDTA and DTT yields a more uniform size distribution of 16–22 nm (scale bar is 50 nm). (d) Individual particles from c at higher magnification (scale bar is 25 nm).

XAS and EPR spectroscopy of IssA-bound iron and sulfide

XAS was conducted at both Fe K-edge and S K-edge absorption energies to characterize the iron and sulfur bound by IssA. Figure 3a compares the X-ray absorption near-edge spectra of IssA with a number of different iron–sulfur proteins. For both the S and Fe K-edges, the spectra strongly resemble those of the linear [3Fe-4S]+ cluster in the high pH form of the enzyme aconitase15. The sulfur K-edge data of IssA show two pronounced peaks, one at low energy at 2467.1 eV, and a broader less-well defined feature at 2470.2 eV. The former, lower energy absorption, is characteristic of sulfide co-ordinated to ferric ions, arising from dipole-allowed transitions of the 1 s electron to unfilled molecular orbitals involving both sulfur 3p and metal 3d orbitals, with vacancies due to covalency of the Fe–S bond 16,17. The second, higher energy transition is attributable to other types of sulfur in the system, such those in the one cysteine and four methionine residues in the IssA monomer (see below). In both S and Fe K-edge cases, the IssA spectra are highly characteristic of a Fe(III) oxidation state16,18.

Scheme 3: IssA X-ray absorption spectra.
Figure 3

(a) X-ray absorption near-edge spectra of IssA compared with a number of Fe–S proteins; a: IssA, b: linear 3Fe-4S cluster in human aconitase, c: P. furiosus 3Fe-4S ferredoxin and d: P. furiosus rubredoxin. The feature marked (*) in the IssA spectrum is due to a trace amount of sulfonate buffer. For both the S and Fe K-edge data, the IssA spectrum most resembles that of the linear 3Fe-4S cluster. (b) S and Fe K-edge EXAFS spectra, together with EXAFS Fourier transforms (S–Fe and Fe–S phase-corrected, respectively) showing experimental data (blue lines) together with best fits (red lines), the inset in the Fourier transform figure shows the structure used to compute the multiple scattering EXAFS. Best fits were computed with two S–Fe at 2.239(3) Å, σ2=0.0049(3) Å2 and four Fe–S at 2.243(1) Å, σ2=0.0044(1) Å2, two Fe····Fe at 2.704(1) Å, σ2=0.0032(1) Å2 and two Fe····Fe at 5.408 Å and σ2=0.0064 Å2.

The S and Fe K-edge EXAFS oscillations, together with the corresponding EXAFS Fourier transforms, are shown in Fig. 3b, with the best fits. The Fe EXAFS data is dominated by intense backscattering from four Fe–S interactions at 2.24 Å, plus backscattering attributable to two Fe····Fe interactions at 2.70 Å. The sulfur K-edge EXAFS, on the other hand, fits to two S–Fe interactions, with no substantial outer shell contributions (Fig. 3b). Experiments at the low X-ray energies of the sulfur K-edge are more challenging than at the iron K-edge, and for this reason the latter data are of substantially better signal to noise ratio. The iron EXAFS also shows a second less intense outer shell Fourier transform peak at 5.4 Å, twice that of the shorter Fe····Fe interaction. This fits well to a long-range Fe····Fe interaction once multiple scattering interactions for a linear arrangement of iron atoms are included. This 5.4 Å interaction is close to the limit of the noise, being about three times the transform peak height of the noise as estimated from higher R values, using data to 18 Å−1. However, it shows behaviour characteristic of real EXAFS, rather than a noise peak in the Fourier transform, so that the feature persists irrespective of the k-ranges, and moreover fits to a very similar Fe····Fe distance with different k-ranges. The use of multiple scattering EXAFS reproduces many weaker features in the EXAFS (Fig. 3b) that also appear to be above the noise level (Supplementary Fig. 5). Other weak interactions in the EXAFS data, such as the 3.3 Å feature in the S K-edge EXAFS, do not behave in this manner and these are likely due to noise. Similar long-range Fe····Fe interactions have previously been observed in the Fe K-edge EXAFS of aconitase containing a linear [3Fe-4S]+ cluster15.

Taken together these XAS data indicate that IssA contains a linear (FeS2)n polymer with two sulfur atoms bridging each pair of Fe(III) ions. Compounds with such a structure are known as thioferrates19,20. They have been synthesized intentionally21 and unintentionally22 and occur naturally as the mineral erdite23, however, this is the first time they have been found in a biological system. Thioferrates contain Fe3+ and S2− in anionic chains of edge-sharing FeS4 tetrahedra separated by charge-balancing cations (Fig. 4), and the oxidation states and atomic structure are in agreement with our conclusions for IssA based on XAS. In agreement with this hypothesis, the sulfur K near-edge spectrum of CsFeS2 has been reported17, and shows a distinctive low-energy peak at 2467.0 eV (allowing for the different energy calibration used by Rose et al.17). Other mineral forms of iron sulfide are inconsistent with the EXAFS because they would show more than two short-range Fe····Fe interactions and would lack the observed long-range 5.4 Å Fe····Fe interactions. Moreover, discrete clusters such as the aconitase linear [3Fe-4S] cluster would require protein-based external thiolate donors that are not present in IssA (it contains only one Cys residue/monomer, see below). We therefore conclude that IssA contains polymeric ferric sulfide with a thioferrate-type (FeS2)n structure. This is also supported by the measured acid-labile iron, sulfide and sulfane sulfur content of IssA (38 Fe, 38 S2− and 17 S0 atoms/monomer). Degradation of synthetic thioferrates in acid (the conditions used for the assays) has been shown to produce Fe2+, S2− and S0 in a 2:3:1 ratio22, which explains the detection of ‘S0’ in a 1:2 ratio with iron. Since S0 will also be produced under the acidic conditions of the S2− assay, formation of polysulfides further reduces the amount of sulfur available for detection as H2S (ref. 24).

Scheme 4: Thioferrate structure.
Figure 4

(a) Inorganic potassium thioferrate (KFeS2) supercell of the crystal structure using three times the crystallographic c axis. Potassium is shown as purple spheres, iron as red spheres and sulfur as yellow spheres. Sections of five linear FeS2 chains are visible. (b) A five Fe section of a single thioferrate chain, the proposed Fe–S component of IssA.

IssA exhibits a very broad, isotropic-type EPR signal spanning 3,000 Gauss and centred around g2.2. It is only observable above 60 K and increases in intensity with increasing temperature (Supplementary Fig. 6). At 60 K, the spectra show weak resonances centred around g values of 4.3 and 2.0, which increase in intensity with decreasing temperature. The g=4.3 signal is indicative of trace amounts of adventitiously-bound high-spin (S=5/2) Fe3+ or magnetically isolated linear [3Fe-4S]+ clusters (S=5/2)4. The origin of the weak signal in the g=2 region (<0.01 spin/IssA monomer) is unknown. The anomalous temperature dependence of the very broad isotropic signal is unique among protein-derived EPR signals, but is in agreement with the EPR signal observed for synthetic thioferrates20,25. Generally, EPR signals increase in intensity with decreasing temperature, however, the antiferromagnetic coupling between high-spin Fe3+ ions in thioferrate polymers results in decreased intensity at temperatures below the Néel temperature26. At elevated temperature, thermal disordering of the orientation of electron spins results in increased net magnetization and synthetic thioferrates yield a broad, isotropic EPR signal centred near g=2 (refs 20, 25).

In vitro IssA-mediated reconstitution of apo-Fd

To test the hypothesis that IssA functions as a storage protein for Fe and S that can be used for the assembly of iron-sulfur clusters, the ability of IssA to reconstitute the [4Fe-4S] cluster in the apo-form of P. furiosus ferredoxin (Fd) was investigated. P. furiosus almost exclusively contains [4Fe-4S] cluster-containing Fe–S proteins, and P. furiosus Fd is an abundant protein that is used as electron donor for numerous enzymes. Reconstitution experiments were carried out anaerobically under a variety of conditions. IssA with stoichiometric or a twofold excess of bound Fe and S (as thioferrate) was mixed with apo-Fd at pH 6.8 (the physiological pH for P. furiosus growth) and incubated at room temperature for 24 h or at 80 °C for 1 h. Since a [4Fe-4S]2+ cluster is more reduced than the all-Fe3+ thioferrate iron and sulfide donor, sodium dithionite (Em−420 mV versus NHE), DTT (Em−330 mV versus NHE), or tris(2-carboxyethyl) phosphine (TCEP; Em −280 mV versus NHE) were included in the reaction mixture as a reductant, a disulfide-cleaving reagent (DTT and TCEP) and a dithiol-metal chelating agent (DTT). After centrifugation to remove unreacted IssA, the Fd was purified and the cluster content and integrity were assessed compared with native holo-Fd based on ultraviolet–visible absorption and CD spectra quantified based on protein determinations. No reconstitution occurred in the presence of dithionite or TCEP. However, reconstitution of the [4Fe-4S]2+ cluster was observed in the presence of DTT (Fig. 5). Reconstitutions with a twofold excess of IssA-bound Fe and S resulted in 50±5% cluster incorporation after incubation with IssA for 24 h at room temperature. Cluster incorporation was greatly accelerated at more physiologically relevant temperatures, with 45±5% cluster incorporation after incubation with near stoichiometric IssA Fe and S at 80 °C for 1 h. These results provide in vitro evidence that the IssA thioferrate core can provide iron and sulfide for [4Fe-4S] cluster assembly in P. furiosus. They also suggest that in vivo an as yet unknown thiol(s), that is replaced in vitro by DTT, plays a role in disassembling the thioferrate polymer into transferrable pieces by chelating Fe, [2Fe-2S]2+, or linear [3Fe-4S]1+ fragments under reducing conditions that are then assembled into [4Fe-4S]2+ clusters in acceptor proteins such as Fd.

Scheme 5: IssA-mediated reconstitution of a [4Fe-4S]2+ cluster.
Figure 5

Reference UV-visible and CD spectra for holo- and apo- forms of P. furiosis Fd are shown by black and magenta lines, respectively. Repurified Fd products of IssA-mediated reconstitution of apo-Fd in the presence of DTT are shown in red (room temperature for 24 hours) and blue (80 °C for 1 hour).

Phylogeny of IssA is distinct from Nif-related families

IssA from P. furiosus is composed of 179 amino acids and has a predicted molecular weight of 19 kDa from its gene sequence (PF2025). The N-terminal 109 residues comprise an IPR003731 InterPro globular domain27 while the C-terminal residues form a tail region that contains the one cysteine residue in the protein. The globular domain has been identified in over 5,000 proteins widely distributed throughout Archaea and Bacteria. These include many hypothetical proteins as well as members of the NifB, NifX and NafY protein families that function in the maturation of the iron–molybdenum–sulfur (Fe–Mo–S) cluster used by nitrogenase. A cladogram of all proteins containing the IPR003731 domain (Fig. 6a) clearly differentiates a NifX clade (84% bootstrap confidence) and a closely related NafY clade (100% confidence). NifB proteins, which also contain a radical-SAM domain, form a distinct clade (92% confidence) that is the most distantly related of the Nif proteins to the IssA clade (78% confidence), which is based on the P. furiosus protein.

Scheme 6: IssA bioinformatic analysis.
Figure 6

(a) Cladograms of proteins containing the IPR003731 domain. Coloured clades contain members of the NifB (IPR005980, blue), NifX (IPR013480, turquoise) and NafY (IPR031763, purple) InterPro protein families. The proposed IssA clade (red) is based on predicted protein isoelectric point (pI) and occurrence of proteins with a glycine rich region at the C-terminus. P. furiosus IssA and the homologue from Methanothermobacter thermautotrophicus (MTH1175) are shown by red and yellow arrowheads respectively. (b) Modelled structure of the IssA IPR003731 domain based on the NMR structure of MTH1175 (red). The C-terminal region is shown unstructured, as predicted for the apo-form.

NifB, NifX and NafY all bind complex iron-sulfur clusters and transfer them to other proteins. NifB and NifX bind a precursor (NifB-co)28 in the biosynthesis of the nitrogenase Fe–Mo–S cluster (MoFe7S9C)29, which is bound by NafY. NifB-co is assembled on the IPR003731 domain of NifB with a proposed composition of Fe8S9C (ref. 30). NifB proteins contain many highly conserved cysteine residues that bind the Fe–S cluster precursors to NifB-co. However, most IPR003731 domain-containing proteins (2,948) contain fewer than the four cysteines required to fully ligate an iron-sulfur cluster (Supplementary Fig. 7). Due to sequence diversity in this large InterPro family, there is little broad amino acid conservation. However, members of the IssA clade contain conserved acidic residues suited to binding the ferric iron necessary for thioferrate synthesis. Thus, we propose that the IPR003731 domain in P. furiosus IssA functions as a scaffold for thioferrate assembly and binding, analogous to the role of the homologous NifB domain in NifB-co assembly. Unlike thioferrate, NifB-co likely contains ferrous iron30,31. However according to the proposed Fe6S9C composition30, it is likely an anionic iron–sulfur species like thioferrate (overall charge of −2 or −4 (refs 30, 31)) and may require electrostatic interactions with the protein for stable binding.

The IssA IPR003731 domain binds iron and sulfur

A gene encoding the polyhistidine-tagged globular IPR003731 domain of IssA (residues 1–109) was constructed, expressed in E. coli, and purified by affinity chromatography (apo-IssA) to investigate whether the domain binds iron and sulfide and how this affects oligomerization. When colourless monomeric apo-IssA was incubated with a 40-fold excess of iron (ferrous ammonium sulfate) and sulfur (sodium sulfide), the black product eluted from a Superose 6 SEC column with molecular weights ranging from 200 kDa to 900 kDa with up to 25 Fe per IssA monomer. Hence, the IPR003731 globular domain of IssA clearly binds Fe and S, stimulating oligomerization in vitro, although the product is very heterogeneous and it was not characterized further.

Chemical character and conservation of the IssA C-terminus

The C-terminal tail (70 of 179 residues) of P. furiosus IssA contains a proline-rich region (9 prolines in 23 amino acids; or 11 in 70) followed by a flexible region comprised predominantly of cationic (7 of 70), aromatic (14 of 70) and glycine (15 of 70) residues. Secondary structure prediction32 indicates that this tail is unstructured. We propose that this cationic region (the predicted tail pI is 10.7) is involved in binding thioferrate formed by the N-terminal domain. Many members of the IssA clade (Fig. 6a) also have a positively charged tail region with a high aromatic and glycine content (Supplementary Fig. 7) although the sequence of the tail in the P. furiosus protein is highly conserved only in IssA proteins in species within the Thermococcales. Indeed, some members of the IssA clade have a shortened tail (<70 residues) and some have little or no tail region, which includes the IssA-type protein from Methanothermobacter thermautotrophicus that was used to model the structure of the IPR003731 domain of P. furiosus IssA (Fig. 6b). In spite of a lack of high sequence conservation in long-tailed IssA members the prevalence of key residues (tryptophan, glycine and arginine) is conserved and a similar repeating pattern is present thereby conserving the chemical character of the C-terminal region of these IssA-like proteins. This is consistent with their proposed role in binding electrostatically to thioferrate, which is negatively charged along its length and does not require ligands with precise positioning. Interestingly, the cladogram reveals another clade of non-themrophilic IssA homologues (termed IssX, Supplementary Fig. 7) that share key features with IssA including a high pI and a glycine rich tail, although none have yet been characterized.


The results presented herein show that IssA is a novel type of protein with a unique role in iron–sulfur metabolism. As indicated by TEM analysis of natively purified protein, it assembles into massive polymeric nanostructures reaching 300 nm in size. XAS analyses indicate that Fe and S are bound to the protein in thioferrate-type linear chains of ferric sulfide (Figs 3 and 4). While other large monomeric metalloprotein complexes are known (12 nm diameter ferritin33 and a 30–35 nm diameter calcium-binding piscine betanodavirus capsid34), IssA forms much larger particles and by far the most iron of any complex known (96,000 in a 300 nm string of 20 nm spheres), though less iron per kDa of protein than ferritin (1.8 Fe/kDa versus 9.6 Fe/kDa, respectively)8. Treatment of purified IssA with guanidinium, EDTA and DTT in an aerobic environment leads to homogeneous, spherical particles of 20 nm diameter (Fig. 2b), which may comprise a basic unit of assembly for larger structures observed in vivo (Fig. 1a,b) and in vitro (Fig. 2a). These treatment conditions are capable of destabilizing protein structure as well as likely degrading thioferrate through chelation of ferric iron by EDTA and removal of sulfide to form DTT persulfide from DTT disulfide. Thus it seems likely that thioferrate is involved in holding these 20 nm units together in the larger nanostructures. A 20 nm sphere would be expected to contain approximately 6,400 Fe atoms and 170 copies of the 19 kDa polypeptide. Ferrihydrite iron, found in ferritin, is accessible to iron removal due to its low crystallinity and high specific area9. Similarly, thioferrate iron may be relatively accessible due to its one-dimensional structure, requiring fewer bond scissions for iron removal than three-dimensional minerals.

We have also demonstrated that IssA can provide Fe and S for assembly of [4Fe-4S]2+ clusters in apo-Fd from P. furiosus under anaerobic conditions in the presence of DTT (Fig. 5). The mechanism of this process has yet to be determined, but likely involves the ability of DTT (in vitro) or a cellular thiol (in vivo) to bind Fe3+ and/or [2Fe-2S]2+/[3Fe-4S]1+ thioferrate fragments (Fig. 4). For example, DTT is known to ligate Fe3+ and Fe2+ (ref. 35) and the non-cysteinyl-ligated sites of protein-bound [4Fe-4S]2+ clusters36. Moreover, a [2Fe-2S]2+ or [3Fe-4S]+ fragment of thioferrate could theoretically be obtained from IssA by simple ligand exchange if appropriately activated (for example, by reduction), and both clusters can be readily converted to [4Fe-4S]2+ clusters in biological and in synthetic chemistry37,38. The conversion of two [2Fe-2S]2+ clusters to generate a [4Fe-4S]2+ cluster occurs via two-electron reductive coupling37,38, and this reaction is believed to be involved in de novo cluster assembly in the ISC system38,39. In aconitase and in synthetic chemistry, linear [3Fe-4S]1+ clusters can be converted to a [4Fe-4S]2+ cluster by the addition of Fe2+ and one electron37. While we have yet to determine the physiological mechanism for Fe–S cluster assembly in P. furiosus, the in vitro cluster assembly results presented here, coupled with the high Fe and S content of IssA and its iron- and sulfide-dependent expression, strongly support a role for IssA in storing Fe and S that can be used for the biosynthesis of Fe–S clusters. Moreover, the in vitro results raise the possibility of spontaneous [4Fe-4S] cluster assembly from Fe3+,2+ and S2− on acceptor proteins under Fe and sulfide replete conditions in some strictly anaerobic hyperthermophilic archaea such as P. furiosus. Except for SufCBD and two putative SufS cysteine desulfurases, P. furiosus does not encode any other known Fe–S cluster assembly protein. Moreover, SufC and SufD contain no cysteine residues and the putative SufB scaffold protein has only two cysteines (compared to 13 in E. coli SufB), which are both rigorously conserved in other SufB proteins. Hence, the scaffolding hypothesis that constitutes the current paradigm for Fe–S cluster assembly39, may not apply in P. furiosus and related organisms.

Because IssA is only produced in P. furiosus when the organism is grown in the presence of abundant iron and sulfide, we propose that the thioferrate structure is synthesized directly from these inorganic precursors. The fact that apo-IssA binds Fe and S from inorganic salts also supports this idea. Interestingly, expression of the genes encoding the two cysteine desulfurase homologues in P. furiosus (PF0164 and PF1066) are strongly down-regulated (5.1- and 3.2-fold) in response to S0, while the sufBD homologues, which are likely to be involved with some aspect of Fe–S cluster trafficking, are strongly upregulated along with issA when S0 is present12. Thus, sulfide itself rather than cysteine is likely to provide sulfur for Fe–S cluster assembly under conditions of intracellular sulfide production in P. furiosus. Consequently, Fe–S clusters synthesized from thioferrate may require less energy than canonical ATP-driven scaffold-assembled Fe–S clusters.

Binding of inorganic Fe and S to IPR003731 domain of apo-IssA suggests that native IssA binds anionic thioferrate (FeS2)n at the IPR003731 domain and that the tail region stabilizes the structure through electrostatic interactions. The 70 amino-acid C-terminal region of IssA contains 7 cationic residues (mostly arginine) that are sufficiently close to each other to preclude a folded structure without a negatively charged counterpart such as thioferrate. In addition, the abundance of glycine residues further indicates a lack of secondary structure in the absence of thioferrate. Hence we propose a model in which the cationic tail may bind the anionic thioferrate chain by wrapping around thioferrate in perhaps a helical arrangement, which is further stabilized by interactions between the tail’s aromatic residues. This interaction would confer a defined structure on the otherwise disordered IssA tail. Since apo-IssA is purified as a monomer, but oligomerizes in the Fe–S bound state, we suggest that formation of the observed 20 nm spherical IssA particles is also dependent on association with thioferrate. According to the estimated Fe-protein ratio, additional cations are needed to completely balance the negative charge on thioferrate, and we expect these are provided by loosely bound cations as well as the single zinc ion per protein (Supplementary Table 1), which may also play a structural role in nanoparticle formation.


Thin section electron microscopy

P. furiosus was grown as described12. Cells were fixed with 2% paraformaldehyde and 1% glutaraldehyde, dehydrated with ethanol, infiltrated with LR White and polymerized at 50 °C. Final samples were sectioned with a diamond ultratome and placed on nickel grids. Grids were blocked with 50 mM Tris-HCl (pH 7.4), 0.5 M NaCl, 0.05% v/v Tween 20 with 3% w/v bovine serum albumin, then incubated with primary antibody14 (1:10,000) followed by 10 nm gold-conjugated anti-rabbit IgG (1:50). Samples were stained with 2% uranyl acetate; imaging and energy dispersive X-ray analysis was performed using a FEI Technai 20 transmission electron microscope (Center for Advanced Ultrastructural Research, University of Georgia).

IssA aggregate protein analysis

The IssA nanoparticle aggregate was immunoprecipitated from P. furiosus cells grown continuously with elemental sulfur12. Cells were lysed40, DNA was sheared using a 21 gauge needle, and protein concentration was brought to 2.5 mg ml−1. Cell lysates were pre-cleared with rabbit IgG and then immunoprecipitated with purified IssA antibody cross-linked to Protein A magnetic beads according to the manufacturer’s protocol. The immunoprecipitated sample was digested with 10 ng μl−1 trypsin at 37 °C overnight and spotted onto a MALDI Anchor plate according to the manufacturer’s protocol using NuTip C-18 tips with α-cyano-4-hydroxycinnamic acid matrix. The resulting peptide masses were analysed using MASCOT software searching a Pyrococcus furiosus-specific database and allowing for 2 missed cleavages and a mass difference of ±0.4 Da.

Native protein purification

IssA was purified from Pyrococcus furiosus cells grown continuously with S0 (ref. 12). Cells were lysed40 and centrifuged at 100,000g for 1 h. The pellet was washed first in 50 mM Tris-HCl (pH 8.0), 2 mM dithionite and 2 mM DTT (buffer A) containing 1% w/v sodium dodecyl sulfate, then twice in buffer A and applied to a caesium chloride gradient (density=1.4 g ml−1, 260,000 g, 9.5 h) to remove precipitated material from the media. Fractions containing IssA were pooled and dialyzed against 4 l buffer A and concentrated using a Centricon centrifugal concentrator with a 10 kDa cut-off (Millipore). Protein concentration was estimated using the bicinchoninic acid method at 60 °C (ref. 41), following precipitation with 20% w/v trichloroacetic acid. Gel electrophoresis and western blot analyses were performed as described14.

Elemental and chemical analyses

An Agilent 7500c ICP-MS was used to quantify iron and other metals. Sample processing, instrument settings and analysis of mass spectrometry data have been previously described42. Colorimetric assays were used to measure iron43, sulfide12 and sulfane sulfur44 in purified protein.

Negative stain electron microscopy

Purified IssA exists as a suspension that settles in 1 h. TEM was conducted on purified IssA as well as on solubilized samples. Solubilization was achieved by adding 20 μl denaturing buffer (6 M guanidinium chloride, 50 mM Tris, 1 mM EDTA, 50 mM DTT) to 20 μl IssA sample. This mixture was shaken at room temperature for 4 h after which the sample was fully dissolved. We diluted the solution six fold with deionized water (dH2O) and applied 3 μl to a glow discharged carbon-coated TEM grid. The grid was washed with 3 μl dH2O and stained with 3 μl of 2% uranyl acetate. Microscopy was conducted on a JEOL 2010 F TEM operated at 200 kV high tension and 50 kX magnification. 100 electron micrographs were recorded in a Gatan Ultrascan 4 K by 4 K CCD camera. We automatically selected 30,000 raw particles and performed 2D image classification in EMAN 2 (ref. 45). Composition of the complexes observed in solubilized IssA was estimated using the volume of a 20 nm sphere, protein density of 1.37 g ml−1, FeS density of 4.28 g ml−1, a ratio of 38 Fe: 76S: 1 Zn: 1 protein, and the assumption that all volume is occupied by FeS or protein.

XAS data acquisition and analysis

Details of iron and sulfur K-edge data collection are described in Supplementary Methods, and the effects of radiation exposure on the sulfur K-edge signal from IssA are shown in Supplementary Fig. 8. The EXAFS oscillations χ(k) were quantitatively analysed as previously described46 by curve-fitting using the EXAFSPAK suite of computer programs47 using ab initio theoretical phase and amplitude functions calculated using the programme FEFF version 8.25 (ref. 48). No smoothing, filtering or related operations were performed on the data.

EPR analysis

100 mg purified IssA was loaded into a quartz EPR tube and centrifuged for 30 min. at 1,000g. The supernatant was removed and the X-band (9.6 GHz) EPR spectrum was obtained using a Bruker ESP-300E EPR spectrometer equipped with an ER-4116 dual-mode cavity and an Oxford Instruments ESR-9 flow cryostat.

In vitro IssA-mediated reconstitution of apo-Fd from P. furiosus

Apo-Fd from P. furiosus was prepared using the method of Moulis and Meyer49, except that a 2-h incubation with 8% (w/v) TCA at room temperature was required to fully bleach and precipitate the protein. After centrifugation, the apo-protein pellet was dissolved in 200 mM PIPES buffer, pH 6.8, with 1 M NaCl and 1 M KCl, under anaerobic conditions. Apo-Fd (0.25 mM) was incubated with IssA (0.052 mM in monomer, that is, 8:1 ratio of Fe:Fd) in the same buffer for 24 h at room temperature in the presence of 10 mM DTT, 10 mM sodium dithionite or 10 mM TCEP. The experiment was repeated using the same protocol except that apo-Fd (0.58 mM) was incubated with IssA (0.067 mM in monomer, that is, 4.4:1 ratio of Fe:Fd) at 80 °C for 1 h. In all cases the resultant Fd was repurified by centrifugation to remove unreacted IssA, buffer exchanged into 50 mM Tris-HCl buffer, pH 7.8, loaded onto a HiTrap Q column and removed as a single band with a 0-1 M NaCl gradient. After desalting and concentrating by Amicon ultrafiltration with a 3 kDa membrane, purity was assessed to be >95% based on gel electrophoresis and the protein concentration was determined using the Bradford assay. [4Fe-4S] cluster content and integrity were assessed compared to the holo-Fd using ultraviolet–visible absorption and CD spectra with ɛ and Δɛ values based on protein concentrations.

Expression and purification of apo-IssA using E. coli

Details of design, expression and purification of the apo-IssA construct are described in Supplementary Methods.

Apo-IssA reconstitution

IssA reconstitution was carried out by adding ferrous ammonium sulfate (10 mM) and sodium sulfide (10 mM) in buffer A to apo-IssA (0.25 mM) and incubating with shaking for 1 h at 80 °C. Excess iron and sulfide were removed by buffer exchange using a Centricon concentrator (Millipore) with a 10 kDa cut-off.

Phylogenetic analysis and structural model

Details of IPR003731 sequence selection and alignment, phylogenetic tree generation and refinement, construction of the structural model of IssA, and related analyses are described in Supplementary Methods.

Data availability

Additional data that support the findings of this study are available from the corresponding author upon reasonable request.

Additional information

How to cite this article: Vaccaro, B. J. et al. Biological iron-sulfur storage in a thioferrate-protein nanoparticle. Nat. Commun. 8, 16110 doi: 10.1038/ncomms16110 (2017).

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.


  1. 1.

    , , & Structure, function, and formation of biological iron-sulfur clusters. Annu. Rev. Biochem. 74, 247–281 (2005).

  2. 2.

    & Building Fe–S proteins: bacterial strategies. Nat. Rev. Microbiol. 8, 436–446 (2010).

  3. 3.

    , & Monothiol glutaredoxins function in storing and transporting [Fe2S2] clusters assembled on IscU scaffold proteins. J. Am. Chem. Soc. 134, 15213–15216 (2012).

  4. 4.

    , & Biological and synthetic [Fe3S4] clusters. Adv. Inorg. Chem. 47, 1–82 (1999).

  5. 5.

    et al. Reversible cycling between cysteine persulfide-ligated [2Fe-2S] and cysteine-ligated [4Fe-4S] clusters in the FNR regulatory protein. Proc. Natl Acad. Sci. USA 109, 15734–15739 (2012).

  6. 6.

    et al. The role of mitochondria in cellular iron–sulfur protein biogenesis and iron metabolism. Biochim. Biophys. Acta–Mol. Cell. Res. 1823, 1491–15082012.

  7. 7.

    , & Coordination chemistry of microbial iron transport. Acc. Chem. Res. 48, 2496–2505 (2015).

  8. 8.

    , & Ferritins: furnishing proteins with iron. J. Biol. Inorg. Chem. 21, 13–28 (2016).

  9. 9.

    & A surface structural model for ferrihydrite I: sites related to primary charge, molar mass, and mass density. Geochim. Cosmochim. Acta 73, 4423–4436 (2009).

  10. 10.

    & Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100 °C. Arch. Microbiol. 145, 56–61 (1986).

  11. 11.

    , , & Cysteine is not the sulfur source for iron-sulfur cluster and methionine biosynthesis in the methanogenic archaeon Methanococcus maripaludis. J. Biol. Chem. 285, 31923–31929 (2010).

  12. 12.

    , & Insights into the metabolism of elemental sulfur by the hyperthermophilic archaeon Pyrococcus furiosus: characterization of a coenzyme A-dependent NAD(P)H sulfur oxidoreductase. J. Bacteriol. 189, 4431–4441 (2007).

  13. 13.

    , & DNA microarray analysis of the hyperthermophilic archaeon Pyrococcus furiosus: evidence for a new type of sulfur-reducing enzyme complex. J. Bacteriol. 183, 7027–7036 (2001).

  14. 14.

    , , & The elemental sulfur-responsive protein (SipA) from the hyperthermophilic archaeon Pyrococcus furiosus is regulated by sulfide in an iron-dependent manner. J. Bacteriol. 192, 5841–5843 (2010).

  15. 15.

    et al. Human cytosolic iron regulatory protein 1 contains a linear iron−sulfur cluster. J. Am. Chem. Soc. 123, 10121–10122 (2001).

  16. 16.

    et al. Sulfur K-edge X-ray absorption spectroscopy of 2Fe−2S ferredoxin: covalency of the oxidized and reduced 2Fe forms and comparison to model complexes. J. Am. Chem. Soc. 123, 5444–5452 (2001).

  17. 17.

    et al. Investigation of the electronic structure of 2Fe−2S model complexes and the Rieske protein using ligand K-edge X-ray absorption spectroscopy. J. Am. Chem. Soc. 121, 2353–2363 (1999).

  18. 18.

    et al. Ligand K-edge X-ray absorption spectroscopy and DFT calculations on [Fe3S4]0,+ clusters: delocalization, redox, and effect of the protein environment. J. Am. Chem. Soc. 126, 16868–16878 (2004).

  19. 19.

    , & The antiferromagnetic structures of KFeS2, RbFeS2, KFeSe2, and RbFeSe2 and the correlation between magnetic moments and crystal field calculations. J. Solid State Chem. 70, 262–270 (1987).

  20. 20.

    et al. Magnetic susceptibility and ESR study of the covalent-chain antiferromagnets TlFeS2 and TlFeSe2. Phys. Rev. B 65, 014433 (2001).

  21. 21.

    , , & Oxidation by elemental sulfur and coupling of iron/sulfur complexes. Synthesis and structural characterization of (Et4N)4[Fe4S6(SEt)4], a new Fe/S cluster with a linear Fe4 backbone. Inorg. Chem. 30, 1163–1164 (1991).

  22. 22.

    & The nature of green alkaline iron sulfide solutions and the preparation of sodium iron (III) sulfide, NaFeS2. Can. J. Chem. 56, 2797–2802 (1978).

  23. 23.

    & The crystal structure of erdite, NaFeS2·2H2O. Am. Mineral. 65, 516–521 (1980).

  24. 24.

    in Elemental Sulfur und Sulfur-Rich Compounds II (ed. Steudel, R.) 127–152Springer Berlin Heidelberg (2003).

  25. 25.

    & Magnetic properties of potassium dithioferrate: A linear chain antiferromagnet and model compound for the exchange interactions in two-iron ferredoxins. Biochim. Biophys. Acta 286, 26–35 (1972).

  26. 26.

    , & Magnetic behavior of the KFeS2. J. Magn. Magn. Mater. 272–276, 521–522 (2004).

  27. 27.

    et al. The InterPro protein families database: the classification resource after 15 years. Nucleic Acids Res. 43, D213–D221 (2015).

  28. 28.

    et al. NifX and NifEN exchange NifB cofactor and the VK-cluster, a newly isolated intermediate of the iron-molybdenum cofactor biosynthetic pathway. Mol. Microbiol. 63, 177–192 (2007).

  29. 29.

    et al. X-ray emission spectroscopy evidences a central carbon in the nitrogenase iron-molybdenum cofactor. Science 334, 974–977 (2011).

  30. 30.

    et al. The nitrogenase FeMo-cofactor precursor formed by nifb protein: a diamagnetic cluster containing eight iron atoms. Angew. Chem. Int. Ed. 55, 12764–12767 (2016).

  31. 31.

    & The role of X-ray spectroscopy in understanding the geometric and electronic structure of nitrogenase. Biochim. Biophys. Acta–Mol. Cell. Res. 1853, 1406–1415 (2015).

  32. 32.

    , , & JPred4: a protein secondary structure prediction server. Nucleic Acids Res. 43, W389–W394 (2015).

  33. 33.

    et al. Ferritin structure from Mycobacterium tuberculosis: comparative study with homologues identifies extended C-terminus involved in ferroxidase activity. PLoS ONE 6, e18570 (2011).

  34. 34.

    et al. Crystal structures of a piscine betanodavirus: mechanisms of capsid assembly and viral infection. PLoS Pathog. 11, e1005203 (2015).

  35. 35.

    , , , & Spectroscopic and functional characterization of iron-bound forms of Azotobacter vinelandiiNifIscA. Biochemistry 51, 8056–8070 (2012).

  36. 36.

    & Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans. Proc. Natl Acad. Sci. USA 101, 12870–12875 (2004).

  37. 37.

    , & Iron-sulfur clusters: nature's modular, multipurpose structures. Science 277, 653–659 (1997).

  38. 38.

    et al. Formation and properties of [4Fe-4S] clusters on the IscU scaffold protein. Biochemistry 46, 6804–6811 (2007).

  39. 39.

    et al. IscU as a scaffold for iron−sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU. Biochemistry 39, 7856–7862 (2000).

  40. 40.

    et al. Key role for sulfur in peptide metabolism and in regulation of three hydrogenases in the hyperthermophilic archaeon Pyrococcus furiosus. J. Bacteriol. 183, 716–724 (2001).

  41. 41.

    et al. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76–85 (1985).

  42. 42.

    et al. Microbial metalloproteomes are largely uncharacterized. Nature 466, 779–782 (2010).

  43. 43.

    , & Studies on the chemical nature of clostridial ferredoxin. J. Biol. Chem. 238, 3899–3913 (1963).

  44. 44.

    & Polysulfide as a possible substrate for sulfur-reducing bacteria. Arch. Microbiol. 160, 377–382 (1993).

  45. 45.

    et al. EMAN2: an extensible image processing suite for electron microscopy. J. Struct. Biol. 157, 38–46 (2007).

  46. 46.

    , , & The molybdenum site of sulfite oxidase: a comparison of wild-type and the cysteine 207 to serine mutant using X-ray absorption spectroscopy. J. Am. Chem. Soc. 118, 8588–8592 (1996).

  47. 47.

    & EXAFSPAK (2001).

  48. 48.

    & Theoretical approaches to X-ray absorption fine structure. Rev. Mod. Phys. 72, 621–654 (2000).

  49. 49.

    & Characterization of the selenium-substituted 2[4Fe-4Se] ferredoxin from Clostridium pasteurianum. Biochemistry 21, 4762–4771 (1982).

Download references


This research was supported by a grant from the Division of Chemical Sciences, Geosciences and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy (DOE; DE-FG05-95ER20175 to M.W.W.A.) We thank Robert M. Glaeser for providing negative stain electron micrographs and John P. Shields for assistance with whole-cell electron microscopy. Bioinformatic analysis was conducted using the Georgia Advanced Computing Resource Center, a partnership between the University of Georgia’s Office of the Vice President for Research and Office of the Vice President for Information Technology. Work at the University of Saskatchewan was supported by the CRC (G.N.G.), NSERC Canada and CIHR (J.J.H.C is a CIHR-THRUST Associate, M.J.H. held a CIHR PDF award and is a CIHR-THRUST fellow). SSRL is supported by the U.S. DOE and NIH. EPR and IssA-mediated apo-Fd reconstitution studies were supported by a grant from the National Institutes of Health (GM62524 to M.K.J.)

Author information

Author notes

    • Sonya M. Clarkson

    Present address: Conagen Inc., 15 DeAngelo Dr., Bedford, Massachusetts 01730, USA

    • James F. Holden

    Present address: Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003, USA

    • Dong-Woo Lee

    Present address: Laboratory of Applied Biochemistry, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea

    • Mark J. Hackett

    Present address: Department of Chemistry, Curtin University, Perth, Western Australia 6102, Australia

    • Brian J. Vaccaro
    •  & Sonya M. Clarkson

    These authors contributed equally to this work.


  1. Department of Chemistry, University of Georgia, Athens, Georgia 30602, USA

    • Brian J. Vaccaro
    • , Sahel Mohebbi
    •  & Michael K. Johnson
  2. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA

    • Brian J. Vaccaro
    • , Sonya M. Clarkson
    • , James F. Holden
    • , Dong-Woo Lee
    • , Chang-Hao Wu
    • , Farris L. Poole II
    •  & Michael W. W. Adams
  3. Department of Geological Sciences and Chemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5C5, Canada

    • Julien J. H. Cotelesage
    • , Mark J. Hackett
    •  & Graham N. George
  4. Cryo-EM Structural Biology Laboratory, Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan 49503, USA

    • Jingchuan Sun
    •  & Huilin Li


  1. Search for Brian J. Vaccaro in:

  2. Search for Sonya M. Clarkson in:

  3. Search for James F. Holden in:

  4. Search for Dong-Woo Lee in:

  5. Search for Chang-Hao Wu in:

  6. Search for Farris L. Poole II in:

  7. Search for Julien J. H. Cotelesage in:

  8. Search for Mark J. Hackett in:

  9. Search for Sahel Mohebbi in:

  10. Search for Jingchuan Sun in:

  11. Search for Huilin Li in:

  12. Search for Michael K. Johnson in:

  13. Search for Graham N. George in:

  14. Search for Michael W. W. Adams in:


B.J.V., S.M.C., J.F.H., D.-W.L. and M.W.W.A. designed the experiments. B.J.V., S.M.C., J.F.H. and D.-W.L. purified IssA and conducted biochemical analyses. C.-H.W. prepared the recombinant form of IssA. B.J.V. and S.M.C. analysed IssA by TEM in vivo. J.S. and H.L. analysed IssA by TEM in vitro. J.J.H.C., M.J.H. and G.N.G. conducted XAS measurements and data analysis. B.J.V. and M.K.J. conducted EPR analysis. B.J.V. and F.L.P. conducted bioinformatic analyses. M.K.J. designed and S.M. conducted the IssA-mediated Fd reconstitution experiments. B.J.V. wrote the manuscript in collaboration with S.M.C., M.K.J., G.N.G. and M.W.W.A.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Michael W. W. Adams.

Supplementary information


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Creative Commons BYOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit