Correct outer protein shell assembly is a prerequisite for virion infectivity in many multi-shelled dsRNA viruses. In the prototypic dsRNA bacteriophage φ6, the assembly reaction is promoted by calcium ions but its biomechanics remain poorly understood. Here, we describe the near-atomic resolution structure of the φ6 double-shelled particle. The outer T=13 shell protein P8 consists of two alpha-helical domains joined by a linker, which allows the trimer to adopt either a closed or an open conformation. The trimers in an open conformation swap domains with each other. Our observations allow us to propose a mechanistic model for calcium concentration regulated outer shell assembly. Furthermore, the structure provides a prime exemplar of bona fide domain-swapping. This leads us to extend the theory of domain-swapping from the level of monomeric subunits and multimers to closed spherical shells, and to hypothesize a mechanism by which closed protein shells may arise in evolution.
The protein shells of icosahedral viruses assemble with remarkable accuracy from smaller subunits1,2,3. Accumulating evidence suggests that such viruses can be grouped into a fairly small number, perhaps less than a dozen, of viral lineages, each sharing a set of common assembly principles4,5. However, explaining how such closed, highly symmetric shells may have arisen during evolution poses a challenge, as formation of a closed shell first requires a network of complementary subunit-subunit interactions with roughly 50% of the surfaces buried from solvent6. One of the most complex examples of such interactions is the capsid of bacteriophage HK97 where the capsid proteins are intertwined to form a topologically linked ‘chain mail’ organization7. A simpler question to ask is how do multimeric proteins evolve from monomers? It has been suggested that this can be achieved by a mechanism called ‘domain swapping’8,9. In the resulting multimer, the domain-swapped subunits in an ‘open’ conformation recapitulate the domain-domain interactions of the original ‘closed’ conformation of the monomer. This concept explains how mutations increasing the flexibility of a linker region of the monomer can facilitate domain swapping to give rise to the multimer.
Domain-swapping may play a role not just in the assembly and evolution of multimeric proteins, but also of viral shells10,11,12. The structure of rice yellow mottle virus (RYMV; genus Sobemovirus), and its comparison to the related southern cowpea mosaic virus (SCPMV), provides an interesting exemplar12. In SCPMV, the T=3 icosahedral capsid is formed in an usual way by trimers in a compact, closed conformation13. In RYMV, however, the trimers are atypical and adopt an open conformation, swapping domains with the neighbouring trimers and creating long range interactions, to which the increased stability of the capsid has been attributed12. However, it remains unclear whether RYMV trimers can exist in both open and closed conformations.
We use bacteriophage φ6, a member of the Cystoviridae family, as a model system to study the assembly of a complicated multi-shelled virus. The inner T=1 shell, composed of protein P1, forms a dodecahedral polymerase complex (PC) harbouring the three viral dsRNA segments (S, M, and L)14,15, the viral polymerase P2 (refs 16, 17), the hexameric packaging protein P4 (refs 18, 19), and the assembly cofactor P7 (refs 20, 21). Unlike eukaryotic dsRNA viruses, φ6 and other cystoviruses undergo a dramatic conformational change during RNA packaging and replication, from an empty collapsed PC to a fully expanded, packaged PC22,23. The expanded PC is covered by a layer of P8, forming the outer T=13 shell23. This double-shelled particle, or nucleocapsid (NC), is itself covered by a protein-lipid envelope during virion assembly24,25. A remarkable aspect of the φ6 system is that PC and NC particles can be assembled in vitro from purified protein and RNA components26. In addition to φ6 bacteriophage (Cystovirus), many other dsRNA viruses, such as aquareovirus (Aquareovirus)27, mammalian reovirus (Orthoreovirus)28, blue tongue virus (Orbivirus)29,30, rice dwarf virus (Phytoreovirus)31,32, rotavirus (Rotavirus)33, and Banna virus (Seadornavirus)34, form a multi-shelled particle with an inner T=1 and one or more outer T=13 shells. While structures for some of these have been solved to near-atomic resolution shedding light into molecular interactions in the T=1 and T=13 shells27,29,30,33, assembly of the T=13 shell in φ6 NC has remained elusive.
Here we present the structure of φ6 NC to near-atomic resolution using electron cryomicroscopy and single particle analysis. The structure of the outer NC shell reveals an intricate domain-swapped architecture created by P8 trimers. The majority of the P8 subunits (540 of 600) are in an open conformation, swapping domains with their neighbours. The inter-trimer interactions recapitulate the intra-trimer interactions of the closed conformation that were also observed within the NC. These observations not only provide a possible explanation for the calcium-regulated assembly of the φ6 NC particle, but they also constitute an exemplar of bona fide domain swapping in a viral shell, allowing us to propose a mechanism for how such complex shells may have arisen during evolution.
Near-atomic resolution structure of the φ6 nucleocapsid
We determined the structure of φ6 double-shelled particle by electron cryomicroscopy (cryo-EM; Fig. 1; Table 1). A high-resolution cryo-EM data set was acquired by imaging purified φ6 NC samples, some of which had spontaneously lost the RNA genome (Supplementary Fig. 1a). Two-dimensional image classification revealed the morphology of NC particles (Supplementary Fig. 1b). Some particles had lost either fully or partially the outer P8 shell (Supplementary Fig. 1a,b). Complete double-shelled particles were used to calculate a three-dimensional reconstruction using a ‘gold-standard’ refinement approach (see Methods; Table 1). The overall resolution of the icosahedrally symmetric areas of the reconstruction was 4.0 Å, as determined by Fourier shell correlation (FSC, threshold=0.143; Supplementary Fig. 1c). In many areas the local resolution was approaching 3.0 Å, while some areas, especially solvent exposed loops and areas around the icosahedral five-fold vertices in the outer protein shell were resolved to a lower resolution (Supplementary Fig.1d–i). The resolution was sufficient to not only trace the course of the polypeptide chain but also to align the sequence to the structure and refine the structure, for nearly all of P1 and P8 residues (see Methods). The P4 hexamers located at the icosahedral five-fold axes of symmetry were not resolved due to the symmetry mismatch (Fig. 1). Density corresponding to the three linear dsRNA genome segments appeared as concentric layers spaced ∼3 nm apart but the detailed organization of the segments could not be addressed using icosahedral reconstruction (Fig. 1b).
Architecture of the nucleocapsid outer shell
The NC structure revealed, consistent with our earlier observations23, a total of 200 P8 trimers forming the outer protein shell, following T=13laevo quasi-symmetry (Fig. 1a). Four types of trimers (P8Q, P8R, P8S and P8T) occupy the different positions in the shell: Q-type trimers around the icosahedral five-fold axis of symmetry, S-type trimers adjacent to the two-fold axis, T-type trimers on the three-fold axis and R-type trimers between the other trimers. Each of the 60 asymmetric units of the shell thus consists of 10 P8 chains, as the P8T trimers contribute only one chain to each asymmetric unit.
The T=13 shell revealed a remarkable domain swapped organization of P8 trimers (Fig. 2). The relatively high resolution of the reconstruction allowed modelling the fold of the P8 structure (residues 3–147; Fig. 2a,b; Supplementary Fig. 2). The structure is α-helical and can be divided into two domains that are connected by an elongated linker (Fig. 2c,d): an N-terminal peripheral domain (PD; residues 1–83) is followed by a 10-residue long elongated loop (residue 84–93) and a C-terminal core domain (CD; residues 94–149). The loop ends in a proline residue (Pro93), which breaks the preceding helix α6 of the CD (Supplementary Fig. 2). The CDs intertwine together to form the core of the P8 trimer. The PDs, due to the elongated nature of the linker, are located a considerable distance (∼50 Å) from the CD. Instead of interacting with the CD of the trimer they belong to, for the majority of the P8 molecules the PDs swap between the trimers, each PD interacting with the CDs and PDs of adjacent trimers via heterotypic interactions (Fig. 2e,f). Nine such structures are observed in the icosahedral asymmetric unit of the NC.
In addition to the ‘open’ conformation described above and adopted by 9 out of 10 of the P8 chains, a fundamentally different conformation is also found on the shell. This ‘closed’, more compact, conformation is observed in one of the three chains of the peripentonal P8Q trimer (Fig. 2g; Supplementary Fig. 2). The two conformations differ in degree of bending of the elongated linker that acts as a hinge. The change in relative position between the two conformations is ∼75 Å and involves a rotation of 71 degrees (Fig. 2h). In the closed conformation, the PD is bound to its own CD via a homotypic interaction (Fig. 2i). This interaction is characterised by an interface that is analogous to the one created between the PD and a CD of an adjacent trimer in heterotypic interactions (Fig. 2j).
P4 hexamer is anchored to the vertices of the P1 shell
In the members of the subfamily Sedoreovirinae of the family Reoviridae, such as blue tongue virus (Orbivirus)29 and rice dwarf virus (Phytoreovirus)32, P-type trimers occupy the five-fold vertices. In contrast, in members of Cystoviridae, such as φ6 (ref. 23) and Pinareovirinae, such as aquareoviruses27, the P-type trimers are absent leaving room for turret-like structures at the icosahedral five-fold vertices. In φ6, the vertices are occupied by the P4 packaging hexamer (Fig. 1). The presence of P4 hexamers at the five-folds provides also a rationale for the observed closed conformation of certain peripentonal P8 proteins (Supplementary Fig. 3); if the type P8Q monomers were all in the open conformation, significant clashes would occur between P8Q and P4 (Supplementary Fig. 3b).
The six-fold symmetry of the P4 hexamer and five-fold symmetry of the icosahedral vertex beneath creates a symmetry mismatch, so that the structure of P4 cannot be resolved using conventional icosahedral reconstruction23. To address this we applied the localized reconstruction method17 to P4 hexamers. Local areas corresponding to P4 hexamers were extracted from the NC images and reconstructed as single particles with six-fold symmetry (‘P4 hexamer reconstruction’) or without any symmetry (‘NC vertex reconstruction’). This allowed a low-resolution structure of the P4 hexamer to be determined in situ (Fig. 3). At a resolution of 9.1 Å (FSC=0.143), fitting of a P4 crystal structure (PDB:4BLO)19 into the density was unambiguous (Fig. 3a–c) and allowed us to orient the hexamer relative to the NC vertex (Fig. 3d,e).
A disorder prediction algorithm suggests that the C-terminal residues of P4 are likely to be disordered (Supplementary Fig. 4)35. Indeed, they are missing in the crystal structure and this disordered region has been proposed to attach the P4 to the underlying P1 shell19. However, in earlier low-resolution cryo-EM reconstructions, the P4 hexamers appeared to float on top of the five-fold vertexes of the P1 shell23,36,37 and the connections between these symmetry-mismatched components have remained unresolved. Our localized reconstruction of P4 hexamers that correctly dealt with the symmetry mismatch revealed elongated densities extending from 3 of the 6 P4 C-terminal parts (Fig. 3f). We hypothesised that these may correspond to the C-terminal parts that are disordered in the crystal structure. Consistent with this hypothesis, in the high-resolution icosahedral NC reconstruction we observed sixty copies of elongated density that lacked any apparent secondary structure stretching ∼115 Å on top of each of the asymmetric P1 dimers (Figs 1b and 4c–e). At the vertex distal part of this density, densities consistent with the side-chains 324-ProArgArg-326 of the P4 C terminus were identified (Fig. 4f), allowing us to specifically assign this density to the C-terminal residues 292–332 of P4. The sixth, unbound C-terminal region of P4 was not resolved in any of the reconstructions, and is likely to be either disordered or unresolved in our maps due to limited resolution of the localized reconstructions.
Binding of up to five of the six flexible C-terminal tails of P4 provides an elegant solution to the symmetry mismatch. These tails are likely to be disordered in solution (Supplementary Fig. 4) and to order only upon binding to P1 during assembly. The P4 tails are analogous to the ‘stay-cables’ observed earlier in adenovirus fibres38. As such cables have now been observed in at least two unrelated viruses, adenovirus (a dsDNA virus)38 and φ6 (a dsRNA virus, this study), they may be a general solution to the problem of attaching fibres and other symmetry-mismatched appendices to vertices of icosahedral virus capsids.
P4 C-terminal tail solidifies the dimeric P1 building block
The inner P1 shell is composed of 60 copies of asymmetric P1 dimers, formed by P1A and P1B (see Fig. 1b)23. A large-scale expansion of the P1 shell occurs during RNA packaging to empty particles and subsequent RNA replication22,23. To model the structural transitions in the P1 shell during this expansion, we refined the existing atomic coordinates of the P1 monomer (PDB:4K7H)39 into our EM density map of the NC (Fig. 4a,b) and compared them to P1 coordinates flexibly fitted to an unexpanded PC reconstruction (PDB:4BTG)39. The angle between the two subunits in the P1A–P1B asymmetric dimer changes relatively little (11°) showing that the P1A–P1B dimer behaves as quite a rigid block during the expansion (Supplementary Fig. 5a,b; Supplementary Movie 1). In contrast to these intradimer angles, the interdimer angles change significantly, mainly at the P1B–P1B interfaces39. The interdimer angle changes 57° at the two-fold interface and 49° at the three-fold interface (Supplementary Fig. 5c,d). The root-mean-square deviation (RMSD) calculated between the two states was 2.6 and 6.0 Å for the P1A and P1B C-alpha coordinates, respectively. This shows that relatively small changes occur within the P1A subunits, while P1B subunits adjust to their local environment in the two very different P1 shell expansion states.
The relative rigidity of the P1A–P1B dimer interface is now explained by our observation that the C-terminal part of P4 bridges the P1A and P1B monomers (Fig. 4c,d) whilst still allowing interdimer angles to change. Thus, the P4 C-terminus seems not only to link P4 hexamers to the P1 shell, but also to play a role in P1 dimerization. A dimer of the major inner capsid protein could indeed be a conserved intermediate in the assembly pathways of dsRNA viruses. This notion is supported by the structure of the Penicillium chrysogenum virus in which the equivalent building block is formed by a covalently linked major capsid protein dimer, produced by gene duplication40. In φ6, we propose that the C-terminal tail of P4 provides an analogous linkage.
When combined with earlier structural and biochemical results, our NC structure allows us to propose an assembly model for φ6 (Fig. 5; Supplementary Movie 2). The assembly of the P1 shell is initiated in vitro by P4 hexamers26 and the C-terminus of P4 is essential for the formation of recombinant PC in vivo41. We now have a structural rationale for these observations, since the P4 C-terminus cements the P1 asymmetric dimer together (Fig. 4c,d). Accordingly, we suggest that the φ6 PC assembly starts with the formation of a P1A–P1B dimer stabilized by the C-terminus of P4 (Fig. 5a). As observed in the P1 crystal structure, individual P1 chains tend to create a pentamer in a conformation that resembles a group of five P1A chains in the unexpanded conformation of PC39. Our modelling experiments also showed that six P1A–P1B dimers per P4 would create steric clashes (Supplementary Movie 2). It is thus likely that each P4 grabs five and not more P1A–P1B dimers. Dimers attached to distinct P4 hexamers then interact to eventually form a closed P1-shell (Fig. 5b). The assembly of the compact empty PC is facilitated by the assembly cofactor P7 (refs 20, 26). P7, together with P2, is located at the three-fold symmetry axis of the empty PC21, but the order of P2 and P7 binding to the PC remains unclear. The PC subsequently packages and replicates the RNA genome segments leading to expansion of the PC (Fig. 5c)23,39 and finally formation of the double-shelled particles by incorporation of the P8 layer (Fig. 5d).
P8 shells form spontaneously in the presence of 0.1–1.0 mM Ca2+ and in the absence of any other structural proteins42. Conversely, depleting calcium by a chelating agent or exposure to low pH leads to P8 shell disassembly42,43. Raman spectroscopy has revealed that the assembled shell stabilizes the mostly α-helical conformation of P8 (ref. 44). It is thus clear that Ca2+ regulates the conformational state of P8. In the light of our observation of a closed and open state of the P8 trimer in the NC, we hypothesize that Ca2+ induces transition of the P8 trimer from the closed to the open conformation and this further facilitates outer shell assembly by domain swapping (Fig. 5d,e). Due to steric clashes, the P8 monomers closest to the P4 hexamers are forced to remain in closed conformation (Supplementary Fig. 3). Ca2+ regulation of P8 conformation, possibly in addition to acidic pH, might also be important to facilitate the delivery of the PC to the host cell upon infection.
The conformational space sampled by P8 trimers in solution and at different Ca2+ concentrations remains unknown. Insolubility of purified P8 and its tendency to readily form large assemblies even in the presence of Ca2+-chelating agents has impeded its solution structure studies by small angle X-ray scattering (Z.S. and J.T.H., unpublished observations). The P8 CD domain is preceded by Pro93 that breaks the helix α6 of the CD. Proline residues are abundant in the linker regions of domain swapped proteins and may play a role in modulating the equilibrium between an open and a closed state45. It is thus possible that Pro93 has a functional role in adding strain to the backbone of the linker region but this hypothesis remains to be tested.
The observed P8 shell assembly allows us to extend the concept of domain swapping to viral shells8,9,10,11,12. Domain swapping is considered bona fide, when the same subunit can still adopt both closed and open conformations, as opposed to the subunits adopting just the open conformation in a multimer and the closed conformation in a different, albeit orthologous, monomeric protein8. Following this nomenclature, our φ6 outer shell structure provides an exemplar of bona fide domain swapping between P8 trimers that exhibit both closed and open conformations.
Analogously to monomer to multimer transition during the evolution of multimeric proteins, our results allow us to propose a hypothetical model how closed viral protein shells may further evolve from such proteins. A primordial, relatively simple capsomer may have some tendency to form larger assemblies, but initially the protein-protein interfaces required to make a spherical shell are not optimized. Mutations in a linker region can increase its flexibility to allow for the formation of an open conformation of the multimeric capsomer. These capsomers in their open conformations would then be able to further link together by recapitulating the domain-domain interactions of the closed conformation. A pre-existing inner protein shell, genome, or internal lipid bilayer present in some viruses, may play a role in increasing the fidelity of shell assembly46. This is also evident in the case of φ6, where in the absence of the inner P1 shell, P8 self-assembles in vitro into aberrant, shell-like structures that vary in size and are not topologically closed43,47. In some viruses, the capsomers may have subsequently lost the ability to form both open and closed conformations. To what extent the outer protein shells of φ6 and other dsRNA viruses are homologous remains an open question, as domain-swapping is not observed in other dsRNA viruses. It is conceivable, however, that linker region deletions and domain-shuffling may have played a further role in stabilizing protein shells. In the case of φ6, the ability of P8 to still adopt both conformations may have been preserved in evolution as this may play a role in calcium-regulated outer shell assembly/disassembly and also the presence of P4 hexamer at the vertices necessitates a closed conformation of P8.
In conclusion, our structural characterization of the φ6 double-shelled particle revealed interactions of the inner P1 shell and symmetry-mismatched P4 packaging hexamers. Most importantly, the study led to the discovery of two states of the outer shell forming protein P8, ‘open’ and ‘closed’. Trimers in the ‘open’ conformation swap domains between each other. This process is possibly regulated by calcium ion concentration but further studies are required to reveal the exact mechanism of Ca2+ regulation. The derived outer shell assembly model not only provides insights into assembly in this particular model system, but also suggests how closed protein shells may have arisen during evolution. Further studies are required to assess to what extent domain swapping may have played a role in other viral systems.
Cryo-EM sample preparation
Bacteriophage φ6 was grown on its host Pseudomonas syringae pv.phaseolicola HB10Y48 and purified as described previously49. The viral envelope was removed by Triton X-114 extraction49 and the resulting aqueous mixture containing the NC loaded onto a CIM DEAE-1 tube monolithic column (BIA Separations, Slovenia) in 20 mM potassium phosphate pH 7.2, 1 mM MgCl2, 0.1 mM CaCl2, 150 mM NaCl from which the bound NC particles were eluted using a 0.15–2 M NaCl complex gradient (in 20 mM potassium phosphate pH 7.2, 1 mM MgCl2, 0.1 mM CaCl2). The NC containing fractions were concentrated using an ultra-centrifugal filter with a 100-kDa cutoff (Amicon; EMD Millipore, Billerica, MA, USA). A 3-μl aliquot of sample, diluted in buffer (20 mM KPO4, pH 7.2, 1 mM MgCl2, 0.1 mM CaCl2, 150 mM NaCl) to concentration of 3 mg ml−1, was applied to a glow-discharged grid (C-flat; Protochips, Raleigh, NC) and vitrified by plunge-freezing into liquid ethane using a Vitrobot (FEI, Hilsboro, OR).
Data acquisition and processing
Data were acquired using a 300-kV transmission electron microscope (Tecnai F30 ‘Polara’; FEI) equipped with an energy filter (slit width 20 eV; GIF Quantum LS, Gatan, Pleasanton, CA) and a direct electron detector (K2 Summit, Gatan). Movies (22 frames, each frame 0.2 s) were collected at in electron counting mode at dose rate of 5 e− per pixel per s at calibrated magnification of 37,037 × resulting in a total dose of ∼16 e− per Å2 and pixel size of 1.35 Å. Data acquisition statistics are summarized in Table 1.
Movie frames were aligned to account for drift50 and contrast transfer function (CTF) parameters were estimated51. A total of ∼900 movies, showing minimal drift and astigmatism were used to pick 16,466 particles. Particles were extracted for 2D reference free classification in Relion 1.3 (ref. 52). Particles from classes showing complete P1 and P8 layers were selected for 3D classification using a previously published NC structure (EMD-1,206) as an initial model. A subset of 13,291 particles extracted from movies was refined using standard refinement and particle polishing protocols implemented in Relion.
The structure of the P4 hexamer was calculated using localized reconstruction ( www.opic.ox.ac.uk/localrec)17. Briefly, twelve ‘subparticles’, each corresponding to a projection of the P4 hexamer in each of the particle images, were extracted (total of 159,463 subparticles) to a smaller box and treated as independent single particles. To assign the orientation for each subparticle, one of the five possible triplets of Euler angles that were equivalent for the corresponding five-fold symmetric vertex was chosen randomly so as not to bias orientations in further processing. Two types of reconstructions were calculated. For the first reconstruction (‘P4 hexamer’), partial signal subtraction was used to remove the contribution of unwanted NC density in the particle images53. We modified our earlier localized reconstruction method17 to allow subtracting ‘all-but-one’ P4 hexamer density (available from http://github.com/OPIC-Oxford/localrec), in addition to the other unwanted densities (P1 and P8 shells and RNA) from the icosahedral reconstruction. Only subparticles from the edge of the particle (total of 103,482; maximum 40° deviation from a side view) were chosen for further analysis to reduce the overlap of the projected hexamer density with the RNA component of the virion that could not be accurately subtracted from the particle images. The structure of the P4 hexamer was then determined using Relion and 3D classification, using a localized reconstruction of the hexamer, calculated from a subset of subparticles and filtered to 40 Å resolution, as a starting model. 3D classification revealed the hexamer in different orientations relative to the five-fold vertex. Particles from classes showing clear hexamer density were combined and six-fold symmetry was applied on the reconstruction. For the second reconstruction (‘NC vertex’), subparticles extracted from the original particle images and orientation parameters from the P4 hexamer reconstruction were used and no symmetry was applied.
Resolutions of the icosahedral NC reconstruction and P4 hexamer localized reconstruction were estimated by using FSC using 0.143 cutoff. The NC vertex reconstruction was low-pass filtered to the resolution of P4 hexamer reconstruction (9.1 Å) and both localized reconstructions were sharpened by applying an inverse B-factor of −600 Å2 (determined by trial and error). Local resolution of the NC reconstruction was estimated using ResMap54. Reconstruction statistics are summarized in Table 2.
Atomic model building and refinement
As no P8 atomic model was available, a polyalanine model was built de novo using Coot. Visually identified densities for bulky residues facilitated building the full atomic model of P8 (L3-Y147) and the C terminus of P4 (R292-L332) with side-chains. The crystal structure of φ6 P1 (PDB:4K7H) was fitted in the NC map using COOT55 as a rigid body in two different positions corresponding to subunits P1A and P1B. P1A and P1B main-chains and side-chains were adjusted using manual and real space fitting in COOT. The crystal structure of φ6 P4 (PDB:4BLO)19 was fitted in the P4 hexamer and NC vertex localized reconstructions as a rigid body in UCSF Chimera56.
Atomic models for all of the chains in the asymmetric unit (P1A, P1B, P4 C terminus and ten copies of P8) were refined in Phenix.real_space_refine applying secondary structure, rotamer, and Ramachandran plot restraints. In addition, non-crystallographic symmetry restraints were applied on P8 chains57. The models were validated with MOLPROBITY58. Atomic model refinement statistics are summarized in Table 3. Disordered regions in P4 sequence (UniProt:P11125) were predicted with RONN35 and changes in subunit-subunit angles calculated in UCSF Chimera59.
Density maps and atomic models that support the findings of this study have been deposited in the Electron Microscopy Database and in the Protein Databank with the accession codes EMD-3571 (NC reconstruction), PDB 5MUU (NC atomic model), EMD-3572 (P4 hexamer reconstruction), PDB 5MUV (P4 atomic model fitted in the P4 hexamer reconstruction), EMD-3573 (NC vertex reconstruction), and PDB 5MUW (P4 atomic model fitted in the NC vertex reconstruction).
How to cite this article: Sun, Z. et al. Double-stranded RNA virus outer shell assembly by bona fide domain-swapping. Nat. Commun. 8, 14814 doi: 10.1038/ncomms14814 (2017).
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We thank Alistair Siebert for electron microscopy support and Riitta Tarkiainen for technical help. The OPIC electron microscopy facility was founded by a Wellcome Trust JIF award (060208/Z/00/Z) and is supported by a WT equipment grant (093305/Z/10/Z). The EU ESFRI Instruct Centre for Virus Production (ICVIR) facility used in this study is supported by the Academy of Finland (grant 272853) and University of Helsinki and this is a collaboration with the Oxford Instruct Centre. This work was funded by Academy of Finland (218080 and 263677 to J.T.H; 283192, 250113, and 272507 to M.M.P.), Sigrid Jusélius Foundation, Medical Research Council (MR/N00065X/1 to K.E.O.), European Research Council under the European Union’s Horizon 2020 research and innovation programme (649053 to J.T.H.) and Wellcome Trust Core Award Grant Number 090532/Z/09/Z. The authors acknowledge the support of employees and the use of experimental resources of Instruct, a Landmark ESFRI project.
Conformational changes in the P1 asymmetric dimer upon polymerase complex expansion. The conformational change in the P1A-P1B asymmetric dimer (A subunit blue; B subunit red) and the changes in the intra-dimer angles at the P1B-P1B interfaces is shown for the icosahedral two-fold and three-fold first from the top and then from the side. The conformational change is shown as a morphing from the polymerase complex (PDB:4BTG) to the nucleocapsid (this study) conformation.
Assembly model of the double-shelled Φ6 particle. 1. Flexible C-terminal tails (dark blue) of P4 hexamer (grey) mediate capture of five P1A-P1B dimers (A subunit blue; B subunit red). Assemblies of P4 plus five P1A-P1B dimers, together with minor virion components P2 and P7 (not depicted) come together to form the assembled polymerase complex (PC) 2. Assembled PC has a shape of a dodecahedron, consisting of 12 assemblies of P4 plus five P1A-P1B dimers. 3. RNA packaging and replication (not depicted) leads to an expansion of the PC particle. 4. Expanded single-shelled polymerase complex 5. P8 trimers (yellow, green, gold, and brown) undergo a conformational change from a 'closed' conformation to an 'open' conformation, allowing them to create the outer shell of the nucleocapsid (NC) by domain-swapping. 6. Assembled double-shelled nucleocapsid particle.
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