Abstract
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.
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Change history
19 December 2013
In the version of this Review Article initially published, a double bond was missing from the chemical structure of the cyclooctene on the left side of the reaction arrow in Figure 1e. The chemical structure has been corrected in the HTML and PDF versions of the article.
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Acknowledgements
We thank K. Rangan and N. Westcott for helpful comments on the manuscript. H.C.H. acknowledges support from Ellison Medical Foundation and US National Institutes of Health–National Institute of General Medical Sciences (1R01GM087544).
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Grammel, M., Hang, H. Chemical reporters for biological discovery. Nat Chem Biol 9, 475–484 (2013). https://doi.org/10.1038/nchembio.1296
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DOI: https://doi.org/10.1038/nchembio.1296
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