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Native Myosin-IXb is a plus-, not a minus-end-directed motor

Nature Cell Biology volume 5, pages 171–172 (2003)Cite this article

Abstract

Myosin-IXb (Myo9b) is a single-headed, processive motor1,2 that contains a Rho-GTPase-activating protein (GAP) domain within its tail3,4. Although tail-less myosin-IXb motor domain moves towards the minus end of the actin filament2, we show here that full-length myosin-IXb is a plus-end-directed motor. This suggests that the tail domain of myosin-IXb regulates motor directionality.

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Figure 1: Native Myo9b moves towards the plus-end of actin filaments.

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Acknowledgements

We thank E. Osterweil for discussions and providing purified Rho, the Pollard laboratory for gel-filtered actin, W. Chang for Myo5a and M. J. Tyska for discussions. This work was supported by National Institutes of Health grants DK-25387 and DK 55389 and American Heart Association grant 9950204N.

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Authors and Affiliations

  1. Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06511, USA

    Christopher B. O'Connell & Mark S. Mooseker

  2. Department of Pathology, Yale University School of Medicine, New Haven, CT 06511, USA

    Mark S. Mooseker

  3. Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA

    Mark S. Mooseker

Authors
  1. Christopher B. O'Connell
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  2. Mark S. Mooseker
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Corresponding author

Correspondence to Mark S. Mooseker.

Supplementary information

Supplementary Methods (PDF 23 kb)

Supplementary Movie 1

Movement of a polarity-labeled filament by native Myo9b. Time lapse images show translocation of a filament with its plus end (labeled red) at the trailing end and minus end leading (green), demonstrating Myo9b is a plus-end-directed motor. Speed ~ 300X. Bar, 5 μm. (AVI 359 kb)

Supplementary Movie 1

Movement of a polarity-labeled filament by Myo5a. As for Myo9b, the red/plus end is trailing, indicating movement is plus-enddirected. Speed ~ 50X. Bar, 5 μm. (AVI 1971 kb)

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O'Connell, C., Mooseker, M. Native Myosin-IXb is a plus-, not a minus-end-directed motor. Nat Cell Biol 5, 171–172 (2003). https://doi.org/10.1038/ncb924

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