Abstract
Formins have been implicated in the regulation of cytoskeletal structure in animals and fungi. Here we show that the formins Bni1 and Bnr1 of budding yeast stimulate the assembly of actin filaments that function as precursors to tropomyosin-stabilized cables that direct polarized cell growth. With loss of formin function, cables disassemble, whereas increased formin activity causes the hyperaccumulation of cable-like filaments. Unlike the assembly of cortical actin patches, cable assembly requires profilin but not the Arp2/3 complex. Thus formins control a distinct pathway for assembling actin filaments that organize the overall polarity of the cell.
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Notes
*Erratum Figs 1–8 were published incorrectly in both the online and print version of Evangelista et al. (Nature Cell Biol. 4, 32–41). These figures have now been replaced online in both the full text and PDF versions, and a corrected print version of the manuscript will be published in the March issue of Nature Cell Biology.
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Acknowledgements
We thank Ruth Collins, John Cooper, Brian Haarer, Tim Huffaker, Daniel Schott, Janet Shaw and Jason Singer for reagents, Sarang Kulkarni for assistance with Deltavision imaging and Bryce Nelson for critical reading of the manuscript. This work was supported by the NIH (GM39066 to A. B.); by grants from NCIC and NSERC to C. B.; and by an NCIC graduate student fellowship to M. E.
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Evangelista, M., Pruyne, D., Amberg, D. et al. Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast. Nat Cell Biol 4, 32–41 (2002). https://doi.org/10.1038/ncb718
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DOI: https://doi.org/10.1038/ncb718
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