Abstract
The 16 kilobase (kb) heterochromatin domain between the chicken β-globin locus and the folate receptor gene is used here to study the roles of RNA-dependent mechanisms and histone modifications in the maintenance of a constitutive heterochromatic structure. Inhibition of histone deacetylase (HDAC) activity is shown to both increase intergenic transcription and render the heterochromatin more accessible to MspI digestion. We show that short interfering RNA (siRNA)-mediated downregulation of the enzyme Dicer has similar effects: histone acetylation is increased, transcript levels rise and the compact chromatin structure becomes more accessible to restriction endonucleases. We also show that the chicken Argonaute 2 homologue binds the 16 kb region in a Dicer-dependent manner and is necessary for a condensed chromatin structure. Heterochromatic domains of this kind, which are widely distributed in vertebrate genomes, thus seem to be maintained in their condensed form by highly conserved mechanisms.
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Acknowledgements
This work was supported by the intramural research program of the NIH, National Institute of Diabetes and Digestive and Kidney Diseases.
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K.G. designed and performed experiments helped write the manuscript; R.G. designed and performed experiments; G.F. designed experiments and helped write the manuscript
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Giles, K., Ghirlando, R. & Felsenfeld, G. Maintenance of a constitutive heterochromatin domain in vertebrates by a Dicer-dependent mechanism. Nat Cell Biol 12, 94–99 (2010). https://doi.org/10.1038/ncb2010
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DOI: https://doi.org/10.1038/ncb2010
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