Missing sequence information for the RNAi vectors used in the article by Janiesch et al. (Nature Cell Biol. 9, 379–390; 2007) is shown below:
RNAi vector construction. RNAi feeding vectors used here were based on the pPD129.36 backbone (a kind gift from A. Fire, SUSM, Stanford, CA). The complete cDNAs of ufd-2 (using the forward primer 5′-ATGATTGAAGACGAGAAAGCAG-3′ and the reverse primer 5′-TTATTTCTTTGAATTTCTTTTCTG-3′) and chn-1 (using the forward primer 5′-ATGTCAAGCGGCGCCGAAC-3′ and the reverse primer 5′-TTAAACCATGCCTCCGGGTTC-3′) were amplified and subcloned into pPD129.36. RNAi constructs that are specific for individual depletion of cdc-48.1 or cdc-48.2, respectively, have been constructed as described previously1 (see also ref. 23 in the article).
References
Yamanaka, K., Okubo, Y., Suzaki, T. & Ogura, T. Analysis of the two p97/VCP/Cdc48p proteins of Caenorhabditis elegans and their suppression of polyglutamine-induced protein aggregation. J. Struct. Biol. 146, 242–50 (2004).
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Addenda. Nat Cell Biol 9, 612 (2007). https://doi.org/10.1038/ncb1586
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DOI: https://doi.org/10.1038/ncb1586
