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Adenoviral preterminal protein stabilizes mini-adenoviral genomes in vitro and in vivo

Nature Biotechnology volume 15, pages 1383–1387 (1997)Cite this article

Abstract

In the absence of host immunity, nonintegrating, first-generation adenoviral vectors remain stable in the nucleus of quiescent transduced cells in mice. A mini-adenoviral genome (9 kb) deleted for viral E1, E2, E3, and late genes, but containing the viral inverted terminal repeats (ITRs), transgene expression cassette (human α1-antitrypsin), and the viral E4 genes was equally efficient at transducing cells in vitro or in vivo as first generation, E1-deleted vectors. In contrast to a first generation vector, gene expression as well as vector DNA was short-lived in cells transduced with the deleted adenoviral genome. We demonstrate that coexpression of the adenoviral E2-preterminal protein from the vector or in trans stabilizes the mini-genome in vitro and in vivo without evidence of cellular toxicity.

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  1. Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA, 98195

    André Lieber, Chen-Yi He & Mark A. Kay

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  1. André Lieber
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Correspondence to Mark A. Kay.

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Lieber, A., He, CY. & Kay, M. Adenoviral preterminal protein stabilizes mini-adenoviral genomes in vitro and in vivo. Nat Biotechnol 15, 1383–1387 (1997). https://doi.org/10.1038/nbt1297-1383

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