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δ-(L-α-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase, the Multienzyme Integrating the Four Primary Reactions in β-Lactam Biosynthesis, as a Model Peptide Synthetase

Bio/Technology volume 11, pages 807–810 (1993)Cite this article

Abstract

ACV Synthetase forms the tripeptide precursor of penicillins and cephalosporins from α-aminoadipate, cysteine, and valine. Catalytic sites for substrate carboxyl activation as adenylates, peptide bond formations, epimerization and release of the tripeptide-thioester are integrated in multifunctional enzymes of 405 to 425 kD. These have been characterized from several pro- and eukaryotic β-Iactam producers. Implications of these results for the thio-template mechanism of peptide formation are discussed, as well as the use of this multienzyme as a model system for enzymatic peptide synthesis.

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Author information

Author notes

  1. Saul Wolfe

    Present address: Chemistry Department, Queen's University, Kingston, Ontario, K7L 3N6, Canada

  2. Jesus M. Cantoral

    Present address: University of Cadiz, Cadiz, Spain

  3. Uwe Fink

    Present address: Max-Volmer Institut, Technical University, Berlin

  4. Andrew MacCabe

    Present address: University of Wageningen, The Netherlands

  5. Harriet Palissa

    Present address: Bundesgesundheitsamt, Berlin

  6. Jinyou Zhang

    Present address: Merck Sharpe & Dohme, Rahway, NJ, USA

  7. Hans von Döhren: Corresponding author.

Authors and Affiliations

  1. Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstr. 29, D-1000, Berlin, 10, F.R.G.

    Uwe Fink, Horst Kleinkauf, Harriet Palissa, Eva Pfeifer, Torsten Schwecke, Henk van Liempt & Hans von Döhren

  2. Tel-Aviv University, Tel-Aviv, Israel

    Yair Aharonowitz & Gerald Cohen

  3. D-6900, Heidelberg, F.R.G.

    Jürgen Bergmeyer

  4. University of Léon, Léon

    Jesus M. Cantoral

  5. Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Arnold L. Demain & Jinyou Zhang

  6. Molecular Genetics Unit, Sir Harold Mitchell Building, Greenside Place, St. Andrews, U.K.

    Jim Kinghorn & Andrew MacCabe

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Aharonowitz, Y., Bergmeyer, J., Cantoral, J. et al. δ-(L-α-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase, the Multienzyme Integrating the Four Primary Reactions in β-Lactam Biosynthesis, as a Model Peptide Synthetase. Nat Biotechnol 11, 807–810 (1993). https://doi.org/10.1038/nbt0793-807

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