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Gene repair validation

Nature Biotechnology volume 19, pages 507–508 (2001)Cite this article

To the editor

An early criticism of PCR-based evidence was that a long-lived chimeraplast might itself act as a primer and, as it contains the “corrected sequence”, skew the result toward high conversion efficiency6. However, this has been discounted by corroborative PCR-independent techniques, including Southern blots of genomic DNA, protein analyses, and functional biochemical assays. Nevertheless, in the April issue of Nature Biotechnology, van der Steege et al.2 emphasize that few studies were coming from laboratories independent of the originators of the technique and proposed an international consortium to freely exchange results and reagents, a proposal that we endorse and to which we can contribute.

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Figure 1: Chimeraplast-directed conversion of apolipoprotein genes.

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Authors and Affiliations

  1. Division of Biochemistry, Royal Holloway, University of London, Surrey, TW20 0EX, UK

    Ian R. Graham, Anna Manzano, Aristides D. Tagalakis, Vanessa Hill, Stuart Beattie & George Dickson

  2. Department of Medicine, Royal Free and University College Medical School, Royal Free Campus, London, NW3 2PF, UK

    Anna Manzano, Aristides D. Tagalakis, Zahra Mohri, Galia Sperber, Silke Schepelmann & Jim S. Owen

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  1. Ian R. Graham
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Graham, I., Manzano, A., Tagalakis, A. et al. Gene repair validation. Nat Biotechnol 19, 507–508 (2001). https://doi.org/10.1038/89209

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