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Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry

Nature Biotechnology volume 16, pages 381–384 (1998)Cite this article

Abstract

Matrix-assisted laser desorption ionization time of flight mass spectrometry was used to sequence exons 5 to 8 of the human p53 gene. A single tube procedure was established for target amplification and mass spectrometric (MS) sequencing. The MS sequencing scheme is designed for high throughput and parallel sample processing, and is amenable to full automation. Reliable sequencing data were obtained using fmol sample amounts. The high resolution and accuracy of MS sequencing was demonstrated by direct sequencing of a heterozygous template.

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Authors and Affiliations

  1. Sequenom, Inc., San Diego, CA, 92121

    Dong-Jing Fu, Kai Tang, Andreas Braun, Dirk Reuter, Brent L. Iverson, Brigitte Darnhofer-Demar, Daniel P. Little, Maryanne J. O'Donnell & Hubert Köster

  2. Boston University, Center for Advanced Biotechnology, Boston, MA, 02215

    Charles R. Cantor

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  1. Dong-Jing Fu
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  2. Kai Tang
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Correspondence to Dong-Jing Fu.

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Fu, DJ., Tang, K., Braun, A. et al. Sequencing exons 5 to 8 of the p53 gene by MALDI-TOF mass spectrometry. Nat Biotechnol 16, 381–384 (1998). https://doi.org/10.1038/nbt0498-381

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  • DOI: https://doi.org/10.1038/nbt0498-381

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