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Cloning of the Bacillus Subtilis IMP Dehydrogenase Gene and its Application to Increased Production of Guanosine

Bio/Technology volume 4, pages 225–228 (1986)Cite this article

Abstract

The gene encoding inosine 5′–monophosphate dehydrogenase (IMP dehydrogenase) of Bacillus subtilis was cloned in pBR322 in Escherichia coli and subsequently subcloned into the B. subtilis plasmid, pC194. The introduction of the recombinant plasmid encoding IMP dehydrogenase to B. subtilis NA6128, an inosine overproducer, elevated its level of IMP dehydrogenase activity and remarkably increased the productivity of guanosine, accompanied by a decreased accumulation of inosine.

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Author notes

  1. Kazuo Nakahama and Masakazu Kikuchi: Biotechnology Laboratories, Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka 532, Japan.

Authors and Affiliations

  1. Applied Microbiology Laboratories, Central Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka, 532, Japan

    Ken-ichiro Miyagawa, Hiroyuki Kimura, Kazuo Nakahama, Masakazu Kikuchi, Muneharu Doi, Shun-ichi Akiyama & Yoshio Nakao

Authors
  1. Ken-ichiro Miyagawa
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  2. Hiroyuki Kimura
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  3. Kazuo Nakahama
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  4. Masakazu Kikuchi
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  5. Muneharu Doi
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  6. Shun-ichi Akiyama
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  7. Yoshio Nakao
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Miyagawa, Ki., Kimura, H., Nakahama, K. et al. Cloning of the Bacillus Subtilis IMP Dehydrogenase Gene and its Application to Increased Production of Guanosine. Nat Biotechnol 4, 225–228 (1986). https://doi.org/10.1038/nbt0386-225

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  • DOI: https://doi.org/10.1038/nbt0386-225

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