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Genotype Specificity of Somatic Embryogenesis and Regeneration in Maize

Bio/Technology volume 4, pages 219–223 (1986)Cite this article

Abstract

Genetic engineering of corn, and other cereals, is currently limited because of the difficulty of regenerating plants via cell culture. Here we report that commercially important inbred lines and hybrids from several different maize groups are capable of being regenerated via somatic embryos. The inbred line A188 has a high frequency of plant regeneration and when crossed into recalcitrant inbreds the resulting F1 hybrids were regenerable. These results demonstrate that nuclear genes exhibiting dominance are important in the formation of somatic embryos and in regeneration of corn plants.

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References

  1. Green, C.E. and Phillips, R.L. 1975. Plant regeneration from tissue cultures of maize. Crop Sci. 15:417–421.

    Article  Google Scholar 

  2. Freeling, M., Woodman, J.C., and Cheng, D.S.K. 1976. Developmental potentials of maize tissue cultures. Maydica 21:97–112.

    CAS  Google Scholar 

  3. Harms, C.T., Lorz, K.H., and Potrykus, I. 1976. Regeneration of plantlets from callus cultures of Zea mays L. Z. Pflanzensuchtg. 77:347–351.

    Google Scholar 

  4. Earle, E.D. 1983. Plant regeneration from cultures of inbred W182BN in N, C, and S cytoplasm. Maize Genetic Coop. Newsletter 57:53.

    Google Scholar 

  5. Green, C.E. 1982. Somatic ernbryogenesis and plant regeneration from friable callus of Zea mays, p. 107. In: Plant Tissue Culture, Proc. 5th Internal Congress Plant Cell and Tissue Culture, Fujiwara, A. (ed), Maruzen Co, Ltd, Tokyo.

    Google Scholar 

  6. Rice, T.B., Reid, R.K., and Gordon, P.N. 1979. In: Propagation of Higher Plants through Tissue Culture-A Bridge between Research and Application, p. 262–277. Hughes, K. W., Henke, R., and Consantin, M. (eds), US. Dept. of Energy.

    Google Scholar 

  7. Sachs, M.M., Lorz, H., Dennis, E.S., Elizur, A., Ferl, R.J., Gerlach, W.L., Pryor, A.J., and Peacock, W.J. 1982. Molecular genetic analysis of the maize anaerobic response, p. 139–145. In: Maize for Biological Research, Sheridan, W. F. (ed), University Press, Grand Forks, ND.

    Google Scholar 

  8. Rhodes, C.A., Green, C.E., and Phillips, R.L. 1982. Regenerable maize tissue cultures derived from immature tassels. Maize Genet. Coop. News Lett. 56:148–149.

    Google Scholar 

  9. Lu, C., Vasil, V., and Vasil, I.K. 1983. Improved efficiency of somatic embryogenesis and plant regeneration in tissue cultures of maize (Zea mays L.). Theor. Appl. Genet. 66:285–289.

    Article  CAS  Google Scholar 

  10. Kamo, K.K., Becwar, M.R., and Hodges, T.K. 1985. Regeneration of Zea mays from embryogenic callus. Bot. Gaz. (in press).

  11. Bingham, E.T., Hurley, L.V., Kaatz, D.M., and Saunders, J.W. 1975. Breeding alfalfa which regenerates from callus tissue in culture. Crop Sci. 15:719–721.

    Article  Google Scholar 

  12. Malmberg, R.L. 1979. Regeneration of whole plants from callus culture of diverse genetic lines of Pisum sativum L. Planta 146:243–244.

    Article  CAS  Google Scholar 

  13. Cummings, D.P., Green, C.E., and Stuthman, D.D. 1976. Callus induction and plant regeneration in oats. Crop Sci. 16:465–470.

    Article  Google Scholar 

  14. Hanzel, J.J., Miller, J.P., Brinkman, M.A., and Fendos, E. 1985. Genotype and media effects on callus formation and regeneration in barley. Crop Sci. 25:27–31.

    Article  Google Scholar 

  15. Keyes, G.J. and Bingham, E.T. 1979. Heterosis and ploidy effects on the growth of alfalfa callus. Crop Sci. 19:473–476.

    Article  Google Scholar 

  16. Bingham, E.T. and McCoy, T.J. 1979. Cultivated alfalfa at the diploid level: origin, reproductive stability, and yield of seed and forage. Crop Sci. 19:97–100.

    Article  Google Scholar 

  17. Saunders, J.W. and Bingham, E.T. 1975. Growth regulator effects on bud initiation in callus cultures of Medicago sativa. Am. J. Bot. 62:850–855.

    Article  Google Scholar 

  18. Reisch, B. and Bingham, E.T. 1980. The genetic control of bud formation from callus cultures of diploid alfalfa. Plant Sci. Lett. 20:71–77.

    Article  Google Scholar 

  19. Izhar, S. and Power, J.B. 1977. Genetical studies with petunia leaf protoplasts I. genetic variation to specific growth hormones and possible genetic control on stages of protoplast development in culture. Plant Sci. Letters 8:375–383.

    Article  CAS  Google Scholar 

  20. Tabata, M. and Motoyoshi, F. 1965. Hereditary control of callus formation in maize endosperm cultured in vitro. Japan. J. Genetics 40:343–355.

    Article  Google Scholar 

  21. Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y. and Bi, F.Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sinica 18:659–668.

    Google Scholar 

  22. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497.

    Article  CAS  Google Scholar 

  23. Green, C.E. and Rhodes, C.A. 1982. Plant regeneration in tissue cultures of maize, p. 367–372. In: Maize for Biological Research (Plant Molecular Biology Assoc), Sheridan, W. F. (ed), Charlottesville, Va.

    Google Scholar 

  24. Sharp, W.R., Sondahl, M.R., Caldas, L.S., and Maraffa, S.B. 1980. The physiology of in vitro asexual embryogenesis. Hort. Rev. 2:268–310.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907

    T. K. Hodges, K. K. Kamo, C. W. Imbrie & M. R. Becwar

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  1. T. K. Hodges
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  2. K. K. Kamo
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  3. C. W. Imbrie
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  4. M. R. Becwar
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Hodges, T., Kamo, K., Imbrie, C. et al. Genotype Specificity of Somatic Embryogenesis and Regeneration in Maize. Nat Biotechnol 4, 219–223 (1986). https://doi.org/10.1038/nbt0386-219

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  • DOI: https://doi.org/10.1038/nbt0386-219

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