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An Isologous Porcine Promoter Permits High Level Expression of Human Hemoglobin in Transgenic Swine

Bio/Technology volume 12, pages 55–59 (1994)Cite this article

Abstract

We describe isologous promoter replacement as an approach to permit high level expression of human hemoglobin in transgenic swine. We linked the human β globin genomic coding region to the porcine β globin promoter and used this fusion gene in an expression construct containing the human β locus control region and the human a and e genes to produce transgenic pigs. The highest level of expression was 24% human (32g/liter) and 30% human α/pig β hybrid (40g/liter) hemoglobin in one transgenic pig. This pig was bred to a non–transgenic animal resulting in the transmission of high level human hemoglobin expression to 5 of 12 progeny.

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Author notes

  1. Ramesh Kumar: Corresponding author.

Authors and Affiliations

  1. DNX Biotherapeutics Inc., 303 College Road East, Princetion, N.J., 08540

    Ajay Sharma, Mike J. Martin, Jeannine F. Okabe, Ruth A. Truglio, Nirpal K. Dhanjal, John S. Logan & Ramesh Kumar

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  1. Ajay Sharma
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  2. Mike J. Martin
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  3. Jeannine F. Okabe
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  4. Ruth A. Truglio
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  5. Nirpal K. Dhanjal
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  6. John S. Logan
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Sharma, A., Martin, M., Okabe, J. et al. An Isologous Porcine Promoter Permits High Level Expression of Human Hemoglobin in Transgenic Swine. Nat Biotechnol 12, 55–59 (1994). https://doi.org/10.1038/nbt0194-55

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