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Negotiating the speed bumps to fluorescence

Two new studies use directed evolution to develop more rapidly maturing fluorescent proteins.

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Figure 1: Representative normalized emission spectra of fluorescent proteins.

References

  1. Nagai, T. et al. Nat. Biotechnol. 20, 87–90 (2002).

    Article  CAS  Google Scholar 

  2. Bevis, B.J. & Glick, B.S. Nat. Biotechnol. 20, 83–87 (2002).

    Article  CAS  Google Scholar 

  3. Tsien, R.Y. Annu. Rev. Biochem. 67, 509–544 (1998).

    Article  CAS  Google Scholar 

  4. Matz, M.V. et al. Nat. Biotechnol. 17, 969–973 (1999).

    Article  CAS  Google Scholar 

  5. Baird, G.S., Zacharias, D.A., & Tsien, R.Y. Proc. Natl. Acad. Sci. USA 97, 11984–11989 (2000).

    Article  CAS  Google Scholar 

  6. Chalfie, M. et al. Science 263, 802–805 (1994).

    Article  CAS  Google Scholar 

  7. Heim, R., Cubitt, A.B. & Tsien, R.Y. Nature 373, 664–665 (1995).

    Article  Google Scholar 

  8. Ormo, M. et al. Science 273, 1392–1395 (1996).

    Article  CAS  Google Scholar 

  9. Wachter, R.M. & Remington, S.J. Curr. Biol. 9, R628–R629 (1999).

    Article  CAS  Google Scholar 

  10. Gross, L.A. et al. Proc. Natl. Acad. Sci. USA 87, 11990–11995 (2000).

    Article  Google Scholar 

  11. Terskikh, A. et al. Science 290, 1478–1479 (2000).

    Article  Google Scholar 

  12. Yarbrough, D., Wachter, R.M., Kallio, K., Matz, M.V. & Remington, S.J. Proc. Natl. Acad. Sci. USA 98, 462–467 (2001).

    Article  CAS  Google Scholar 

  13. Gurskaya, N.G., Savitsky, A.P., Yanushevich, Y.G., Lukyanov, S.A. & Lukyanov, K. BMC Biochemistry 10 July 2001 (http://www.biomedcentral.com/1472-2091/2/6).

  14. Lukyanov, K.A. et al. J. Biol. Chem. 275, 25879–25882 (2000).

    Article  CAS  Google Scholar 

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Remington, S. Negotiating the speed bumps to fluorescence. Nat Biotechnol 20, 28–29 (2002). https://doi.org/10.1038/nbt0102-28

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