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Unexpected CRISPR on-target effects

Nature Biotechnology volume 36, pages 703–704 (2018)Cite this article

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Cas9 can induce extensive on-target damage, including large deletions, inversions, and insertions.

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Figure 1: Outcomes of Cas9-catalyzed DNA double-strand break repair in human cells.

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Author information

Authors and Affiliations

  1. Hyunji Lee and Jin-Soo Kim are at the Center for Genome Engineering, Institute for Basic Science, Daejeon, South Korea.,

    Hyunji Lee & Jin-Soo Kim

  2. Jin-Soo Kim is also with the Department of Chemistry, Seoul National University, Seoul, South Korea.,

    Jin-Soo Kim

Authors
  1. Hyunji Lee
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  2. Jin-Soo Kim
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Correspondence to Jin-Soo Kim.

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Competing interests

J.-S.K. is a cofounder of and holds stock in ToolGen, Inc., a company focused on therapeutic genome editing.

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Lee, H., Kim, JS. Unexpected CRISPR on-target effects. Nat Biotechnol 36, 703–704 (2018). https://doi.org/10.1038/nbt.4207

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