Actin and tubulin are the major components of the eukaryotic cytoskeleton. It was previously thought that prokaryotes lacked a cytoskeleton. However, research in the 1990s firmly established that, in fact, the cytoskeleton originated in prokaryotes, with the discovery that filamentation temperature-sensitive protein Z (FtsZ) is the bacterial homologue of tubulin and MreB is the homologue of actin.
FtsZ is a key player in bacterial cell division and acts at an early point in the pathway. The functional relationship between FtsZ and tubulin was uncertain because they share low sequence identity at the amino-acid level. FtsZ does, however, contain a short tubulin signature sequence, which is known to be involved in the binding of GTP to tubulin. The group of Lutkenhaus initially showed FtsZ to form a filamentous ring structure during cell division and predicted it to be a cytoskeletal element. Then, two independent groups led by Rothfield and Park set out to test whether FtsZ can also function as a GTP-binding protein. Using biochemical assays, they demonstrated that FtsZ purified from bacterial cells can bind and hydrolyse GTP just like eukaryotic tubulin. Furthermore, GTP binding was essential for the function of FtsZ in cell division and also for its propensity to form filaments in vitro, as was shown a little later.
However, the most convincing demonstration that FtsZ was the bacterial counterpart of tubulin came when its crystal structure was solved by Löwe and Amos. They revealed that FtsZ has an overall three-dimensional structure that is similar to eukaryotic α-tubulin and β-tubulin. FtsZ and tubulin also share the way in which they initially assemble into linear polymers with a longitudinal interface between two subunits that contains the GTPase active site.
The first glimpse of filamentous proteins inside bacteria. Arguably the beginning of 'bacterial' cell biology. Jan Lowe
While the connection between FtsZ and tubulin was being established, the origin of actin remained obscure. In 2001, two papers by van den Ent et al. and Jones et al. demonstrated that MreB is the bacterial homologue of eukaryotic actin. Based on sequence similarities, actin belongs to a large superfamily of proteins of unrelated function that includes the chaperone heat-shock protein-70 (Hsp70). Jones and colleagues showed that MreB and the closely related protein Mbl are important for regulating the rod shape of Bacillus subtilis. Using light microscopy, they found that MreB and Mbl form large spirals under the cell membrane. Both proteins contribute to the determination of cell shape. These proteins are conserved, with the existence of homologues in a wide range of bacteria. In general, spherical bacteria lack MreB, whereas those with complex shapes, including rod and filamentous forms, have one or more such genes.
The helical bands observed by Jones et al. were suggestive of a cytoskeleton. van den Ent et al. provided direct evidence for this by showing that purified MreB forms polymers under various conditions in vitro. Electron microscopy showed that the polymers resembled one of the helical strands of filamentous actin. They then determined the crystal structure of MreB and found that it is remarkably similar to actin. These papers provided strong evidence that MreB and actin are homologues.
In the past decade, the discovery of bacterial homologues of actin and tubulin revolutionized our view of bacterial cell architecture. The coming period will address important questions about the function and regulation of the bacterial cytoskeleton, and allow comparisons with the eukaryotic system.
References
ORIGINAL RESEARCH PAPERS
Bi, E. & Lutkenhaus, J. FtsZ ring structure associated with division in Escherichia coli. Nature 354, 161 (1991)
RayChaudhuri, D. & Park, J. T. Escherichia coli cell-division gene ftsZ encodes a novel GTP-binding protein. Nature 359, 251–254 (1992)
de Boer, P., Crossley, R. & Rothfield, L. The essential bacterial cell-division protein FtsZ is a GTPase. Nature 359, 254–256 (1992)
Mukherjee, A., Dai, K. & Lutkenhaus, J. Escherichia coli cell division protein FtsZ is a guanine nucleotide binding protein. Proc. Natl Acad. Sci. USA 90, 1053–1057 (1993)
Löwe, J. & Amos, L. A. Crystal structure of the bacterial cell-division protein FtsZ. Nature 391, 203–206 (1998)
Jones, L. J., Carballido-López, R. & Errington, J. Control of cell shape in bacteria: helical, actin-like filaments in Bacillus subtilis. Cell 104, 913–922 (2001)
van den Ent, F. & Löwe, J. Prokaryotic origin of the actin cytoskeleton. Nature 413, 39–44 (2001)
FURTHER READING
Ausmees, N., Kuhn, J.R. & Jacobs-Wagner, C. The bacterial cytoskeleton: an intermediate filament-like function in cell shape. Cell 115, 705–713 (2003)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nath, D. The prokaryotic cytoskeleton. Nat Rev Mol Cell Biol 9 (Suppl 1), s19 (2010). https://doi.org/10.1038/nrm2582
Published:
Issue Date:
DOI: https://doi.org/10.1038/nrm2582