Abstract
CAG repeat expansion is the cause of an ever-increasing list of neurodegenerative disorders, especially hereditary ataxias. However, genes responsible for 10–50% of the clinically diagnosed ataxias are still unidentified in different populations. Traditional linkage and repeat expansion-detection based methods complemented with human genome sequence and expression information can now accelerate the pace of identification of putative disease candidates. We have analyzed two CAG repeat containing loci, human SMARCA2 and THAP11, which are expressed in the brain as putative candidates for SCAs, using computational as well as polymorphism scanning approaches. Both loci exhibited features characteristic of genes associated with repeat disorders. These loci are polymorphic with respect to size and interruption pattern in the Indian population. Furthermore, computational analysis of glutamine-stretch embedded domains in the respective proteins predicted these regions to be “natively unfolded” beyond a threshold of 40 glutamines. Comparative genome analysis suggested a stabilizing influence of CAA interspersions in repeat tract in THAP11 but not in SMARCA2. Although repeat expansion could not be detected within these genes in unidentified ataxia patients reported in India, we suggest that these loci be screened in other populations, as there is a wide heterogeneity in the prevalence of these disorders in different populations.
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Introduction
Expansion of CAG repeats beyond a threshold of 35–40 glutamines in the majority of patients is a common feature of polyglutamine disorders (reviewed in Cummings and Zoghbi 2000). The nature of the repeat substructure in terms of interruption as well as the length of repeat determines the expandability of trinucleotide repeats (Chung et al. 1993; Eichler et al. 1994; Choudhry et al. 2001). Structurally, long stretches of glutamine encoded by CAA/CAG repeats lead to partial unfolding of the native structure of the protein (Tanaka et al. 2001). Chen (2003) postulated a link between the “natively unfolded” state of the protein and its propensity to cause polyglutamine diseases through a computational approach. His work demonstrated that nine proteins linked to polyglutamine diseases, when confined in a local context, tend to be in an unfolded state.
Sequencing of the complete human genome has revealed ∼2,500 CAG/CTG repeats (n≥5) out of which approximately 1/6th code for polyglutamine (unpublished results). A few of these could be putative candidates for polyglutamine disorders. Prioritization of such loci in the human genome thus becomes mandatory for successful candidate identification. CAG loci implicated in polyglutamine disorders (1) are highly conserved, (2) are polymorphic in normal individuals, (3) sometimes harbor interruptions in the repeat tract, which are lost in expanded alleles, (4) are expressed in the brain, and (5) are prone to aggregation. We studied two loci, SMARCA2 and THAP11, in order to ascertain if they fulfill the above criteria.
SMARCA2, also known as hBRM, is a human homolog of the Drosophila brahma gene (Muchardt and Yaniv 1993; Chiba et al. 1994; Ichinose et al. 1997). The encoded protein is 56% identical and 72% homologous to the D. brahma protein (Wang et al. 1996) and is widely expressed in different tissues, including the brain, as evidenced in the BodyMap database (Hishiki et al. 2000). SMARCA2 and SNF are components of a large protein complex, which may alter the structure of chromatin, allowing other transcription factors to gain access to promoter DNA (Wang et al. 1996). A recent report has demonstrated that SMARCA2 interacts with two ankyrin repeat proteins that are critical components of the Notch signal transduction pathway (Kadam and Emerson 2003).
The protein encoded by the THAP11 gene contains a THAP domain that is a conserved DNA-binding domain with similarity to the DNA-binding domain of Drosophila P element transposases (Roussigne et al. 2003). The locus was identified earlier as a novel triplet repeat-containing gene expressed in the brain (Li et al. 1993, 2003) and is present in the region implicated in SCA4 through linkage study (Flanigan et al. 1996; Hellenbroich et al. 2003; Li et al. 2003).
We reasoned that the presence of long uninterrupted CAG repeats (n≥10) at these loci may predispose the repeat sequence to expand, and therefore, both the genes are putative candidates for neurological diseases exhibiting progressive ataxia.
Materials and methods
Polymorphism studies
Patients demonstrating neurological symptoms diagnosed at the Neuroscience Centre, All India Institute of Medical Sciences, New Delhi, and screened negative for other triplet repeat associated loci were recruited for the study. Polymorphism studies were carried out on 132 and 237 unrelated individuals, including 57 probands who tested negative for the known loci, at SMARCA2 and THAP11 loci, respectively. Informed consent was obtained from all normal and affected individuals before extraction of blood.
The following species of nonhuman primates were used in the analysis of CAG repeats at the two loci: chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla), langur (Presbytis entellus), baboon (Papio hamadryas), rhesus monkey (Macaca mulatta), and bonnet macaque (Macaca radiata). DNA was isolated from peripheral blood leukocytes of human and monkey samples using the modified salting-out procedure (Miller et al. 1988).
Amplification of these loci was carried out using the following primers. SMARCA2: FP—5′-agc cgg ggg ccc tca tcc cag gtg a-3′, RP—5′-cgg ctg ctg ttg ttg ctg cgt ctg t-3′ and THAP11: FP—5′-ggg cgg ccg caa gac cta cac-3′, RP—5′-aag cac ggc cgc gga agc aga tac-3′. One of the primers was fluorescently labeled in each of the amplification reaction. The size of the repeat in the fluorescently labeled PCR products was determined by GeneScan software using an ABI Prism 377 Automated DNA Sequencer (Perkin Elmer, Foster City, CA, USA). Sequencing was carried out using the dideoxy chain terminator chemistry on an ABI Prism 3100 Automated Genetic Analyzer to confirm the repeat size and to determine the nucleotide sequence of the repeats and the flanking region.
Folding/unfolding prediction
Folding/unfolding predictions were based on a previous study (Uversky et al. 2000; Chen 2003) and were performed on ProtParam and ProtScale modules with the ExPASy server (Appel et al. 1994). The amino acid sequence of the SMARCA2 protein was obtained from the SWISS-PROT database (Boeckmann et al. 2003), namely, SN22_HUMAN (accession number P51531), and that of the THAP11 (accession number NP_065190) was obtained from NCBI. ProtParam was utilized for charge calculations, and ProtScale was used to calculate hydrophobicity according to the Kyte and Doolittle protocol (Kyte and Doolittle 1982) with a window size of five residues and normalized to a value between 0 and 1. The mean hydrophobicity (H) is the normalized arithmetic average of hydrophobicity over the range of residues being studied. The global values of mean net charge (R) and mean hydrophobicity were calculated for the full-length (1,586 a.a. residues) and truncated SMARCA2 protein containing 350 residues from N-terminus corresponding to the proline-rich amino terminal domain (Peterson and Tamkun 1995). SMARCA2 protein (accession number P51531) contains 23 consecutive glutamine residues. Computational simulation with and without these 23 consecutive glutamine residues as well as simulated polyQ repeat expansion, with a stepwise increment of five glutamine residues up to 50 glutamine residues was performed both for truncated and full-length SMARCA2 protein. For the computational study of expanded polyQ on THAP11, we focussed on full-length protein (314 a.a. residues) as well as N-terminal truncated protein (a.a. residue 82–314) containing embedded polyQ stretch. The truncated portion (a.a. residues 1–81) is listed as conserved domain in the conserved domain database (CDD) as pfam05485.3, THAP (Marchler-Bauer et al. 2003; Roussigne et al. 2003) and was excluded, as it is likely to fold independently to the rest of the protein. THAP11 protein (accession number NP_065190) contains 29 consecutive glutamine residues. Computational simulation with and without these 29 consecutive glutamine residues as well as simulated polyQ repeat expansion, with a stepwise increment of five glutamine residues up to 50 glutamine residues, was performed both for truncated and full-length THAP11 protein.
Results
CAG repeat polymorphism at SMARCA2 and THAP11 loci
In humans, the CAG repeat at both these loci is polymorphic with a similar unimodal distribution. At the SMARCA2 locus, the CAG repeats are in a range extending from 21 to 31 (Fig. 1a) and have CAA interspersions (Fig. 2a). These interruptions are absent in the middle tract of the repeat, although in a few alleles (7.1%), a CCG triplet was observed.
Similarly, in THAP11, CAG repeats in the range of 20–41 are observed (Fig. 1b) with CAA interruptions. In majority of the cases, variations with respect to both the repeat length and interruption pattern appeared to be polar with changes occurring at the 3′ end of the repeat. The number of CAA interruptions increased with the repeat length. It ranged from two in 20 CAG repeats to four in case of the major allele (29 CAG repeats), which further increased to seven in the largest allele observed (41 CAG repeats) (Fig. 2b).
Comparison of CAG repeat stretch between human and nonhuman primates
Comparison of CAG repeats in the two genes in human and nonhuman primates revealed considerable polymorphism both with respect to repeat length and interruption pattern (Fig. 2). At both loci, the length of the repeat stretch in nonhuman primates is smaller than the most predominant allele (27 in SMARCA2 and 29 in THAP11) in humans. However, the extent of variability in the CAG repeat substructure is different. In the case of SMARCA2, the 5′ and 3′ ends are identical across all species, and variability is observed in the middle tract of the repeat only in humans (Fig. 2a). At the THAP11 locus, the pattern of repeat interruption is highly variable with no similarity between any two species (Fig. 2b). It is noteworthy that more variability in terms of repeat length and interruption pattern is observed at the 3′ end of the repeat in the chimpanzee and humans. In the chimpanzee, the uninterrupted stretch at the 3′ end is much longer than that observed in humans. Additionally, there are variations observed in the regions adjacent to the repeat stretch at both loci in all species (sequence data submitted in GenBank database). CAG repeat length and substructure could not be determined at the SMARCA2 locus in the chimpanzee sample, as the repeat region was refractory to amplification.
Computational analysis of the glutamine-containing domain with respect to folding status
The folded state of the protein was predicted by utilizing Uversky’s algorithm (Uversky et al. 2000), according to which the natively unfolded proteins are specifically localized to a unique region of charge-hydrophobicity phase. A combination of large net charge and low hydrophobicity represents a structural feature observed in the majority of these natively unfolded proteins. Figure 3 represents the mean net charge (R) and mean hydrophobicity (H) for amino acid sequence of the full-length protein and for the domain harboring glutamine repeats. In SMARCA2, exclusion of homogenous 23Q repeat sequence from the full-length protein changed the values from (H)=0.402, (R)=0.005 to (H)=0.406, (R)=0.005, marginally shifting the protein towards the folded state from the unfolded state (Fig. 3a). To examine perturbations in the local domain structure due to long glutamine repeat, similar calculations were done for the amino terminal domain of the SMARCA2 protein. Absence of glutamine repeat stretch from the amino terminal domain of the SMARCA2 protein altered the values from (H)=0.400, (R)=0.008 to (H)=0.420, (R)=0.009, indicating that the presence of long stretches of glutamine may lead to an altered natively unfolded state of the protein. Predictably, simulation with increased glutamine repeat length showed a progressive increase in the natively unfolded state. Full-length SMARCA2 with 50 glutamine repeats is predicted to have (H)=0.396 as against (H)=0.406 for SMARCA2 lacking the polyQ stretch. This small change of hydrophobicity in the context of a full-length protein may not be substantial. However, considering that proteins mostly fold in domains and not as a whole, our analysis of truncated SMARCA2 proline-rich amino terminal domain (N-terminal 350 a.a. residues) demonstrates a large change in net hydrophobicity from (H)=0.420 (without Q stretch) to (H)=0.379 (with 50Q). Local hydrophobic changes of this magnitude are likely to cause localized unfolding and promote aggregation. Similar analysis on full-length as well as truncated THAP11 protein is shown in Fig. 3b. The full-length THAP11 protein with 29 glutamines is “natively folded” (H=0.435), but the protein shifts to a natively unfolded state when the number of glutamines is increased to 50 (H=0.414). A similar simulation study for truncated protein (lacking 1–81 a.a. residues) predicts a large change in net hydrophobicity from (H)=0.470 (folded state) for protein lacking glutamine stretch to (H)=0.399 (unfolded state) for protein with 50Q. As is evident, an increase of glutamine repeats beyond a range of 40 in full-length protein and 35 in N-terminal truncated protein alters the folding characteristics of the protein making it susceptible to misfolding and subsequent aggregation.
Discussion
Using a combination of computational and polymorphism scanning approaches, we analyzed the potential of SMARCA2 and THAP11 genes as plausible candidates for polyglutamine-associated disorders. The presence of a long CAG repeat stretch within the genes has raised the possibility that it might be susceptible to mutation due to repeat expansion. As a first step towards establishing this hypothesis, we determined CAG repeat polymorphism with respect to length and repeat substructure in these genes. Both loci were polymorphic in the Indian population, with identical polymorphism information content (PIC) of 0.38 and a heterozygosity index of 0.43. The low heterozygosities for the above loci do not preclude expansion, as evidenced by earlier studies at the SCA2 locus (Saleem et al. 2000; Choudhry et al. 2001).
It has been extensively studied in SCA1, SCA2, and FMR1 genes (Chung et al. 1993; Eichler et al. 1994; Choudhry et al. 2001) that, besides repeat length, interruption pattern determines the expandability of a locus. Loss of interruptions results in large uninterrupted repeats, which are prone to slippage, subsequently leading to pathogenic lengths. We observed similar variations with respect to the repeat substructure in SMARCA2 and THAP11.
Analysis of comparative DNA fragments from the genome of nonhuman primates encoding SMARCA2 and THAP11 proteins revealed lesser number of repeats in comparison to humans. This is in consonance with a number of reports that indicate directionality towards increase in repeat length during the course of evolution of microsatellites (Gostout et al. 1993; Rubinsztein et al. 1994; Djian et al. 1996). This has also been demonstrated in loci involved in other trinucleotide-repeat-associated disorders.
Comparison of the repeat substructure at the THAP11 locus between humans and nonhuman primates revealed some interesting observations. In case of the chimpanzee, the two interruptions from the 5′ end are invariant, and the 3′ uninterrupted stretch shows considerable length polymorphism. This clearly indicates that slippage occurs at the 3′ end of the repeat, leading to length polymorphisms. In fact, the length of the uninterrupted stretch is considerably longer in the chimpanzee. Length variability at the 3′ end is also observed in humans, but uninterrupted stretches are much smaller compared to the chimpanzee due to the presence of multiple interruptions. The number of interruptions seems to increase with the length of the repeat. The presence of these interruptions thus restricts slippage, resulting in restricted length polymorphism. Other triplet repeat-containing loci also harbor multiple interruptions in longer repeats, which restricts mutability. This again reinforces the fact that THAP11 should be screened in other populations, as it has an expansion-prone repeat pattern.
In SMARCA2, the middle tract contains long and continuous CAG repeats, rendering it a potential site for instability. Long stretches of CAG repeats can generate large alleles through hairpin-mediated slippage, which are prone to expansion (Brahmachari et al. 1995; McMurray 1995). Though CCG interruption is observed in this middle stretch in a few cases, base-pairing rules predict stable hairpin formation and therefore, it is unlikely that CCG interruption will prevent slippage-mediated repeat expansion.
Intranuclear inclusions, the hallmark of polyglutamine-associated disorders, are dependent on the length of glutamine repeats, which promote aggregation. Even though CAA interruptions restrict mutability at the nucleotide level, it could still have phenotypic consequences due to a long stretch of glutamine. Therefore, it becomes imperative to determine the effect of glutamine stretch on protein structure. We used an algorithm developed by Uversky et al. (2000), which uses a unique combination of net charge and mean hydrophobicity to study the effect of glutamines on protein folding. This algorithm predicted the full-length SMARCA2 protein to be natively unfolded and THAP11 to be natively folded. It is quite likely that in the physiological milieu of the cell, binding of ligands and interaction with other proteins may affect the net charge and prevent global unfolding of the protein, but localized unfolding of domains cannot be ruled out. Our analysis indicated that inclusion of a long polyglutamine stretch (n≥35) destabilizes the local context of the protein in both cases, which could lead to local unfolding of the protein thus exposing the glutamine repeats to the surface, which subsequently may result in intramolecular and intermolecular hydrogen bonding (Sharma et al. 1999; Chen 2003). It is possible that repeat lengths greater than 35–40 may promote unfolding, leading to subsequent aggregation and cell death.
In conclusion, our results suggest that SMARCA2 and THAP11 are likely candidates for novel polyglutamine-mediated neurological disorders. However, expansion at these loci in the Indian population was not observed. Since the prevalence of all SCAs is not uniform in all populations worldwide, probands demonstrating characteristic symptoms of polyglutamine diseases should be extensively screened for CAG repeat expansion in the SMARCA2 and THAP11 loci in other populations.
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Acknowledgements
The authors are grateful to Prof. Samir K. Brahmachari for providing intellectual support during the course of this investigation. We are grateful to Deepak Grover and Vikash Kumar for bioinformatics support. We are thankful to Ruchi, Suruchika and N. Makhija for help with GeneScan and sequence analysis. We would like to thank the Primate Research Facilities of the National Institute of Immunology, New Delhi, the Indian Institute of Science, Bangalore, and the Centre for Cellular and Molecular Biology, Hyderabad, India, for providing the primate samples. Financial support from the Department of Biotechnology, Government of India, in the Project on Disease Genomics and CSIR project on “Predictive medicine using repeat and single nucleotide polymorphisms (CMM0016)” is duly acknowledged. Neeraj Pandey and Uma Mittal are grateful to CSIR and UGC, respectively, for Senior Research Fellowship.
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The nucleotide sequence data reported are available in the GenBank database under the accession numbers Brm—AY653188 (Bonnet), AY653189 (Rhesus), AY653190 (Baboon), AY653191 (Langur), AY653192 (Gorilla), Thap11—AY653182 (Bonnet), AY653183 (Rhesus), AY653184 (Baboon), AY653185 (Langur), AY653186 (Gorilla), AY653187 (Chimpanzee).
The first two authors contributed equally to this work.
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Pandey, N., Mittal, U., Srivastava, A.K. et al. SMARCA2 and THAP11: potential candidates for polyglutamine disorders as evidenced from polymorphism and protein-folding simulation studies. J Hum Genet 49, 596–602 (2004). https://doi.org/10.1007/s10038-004-0194-8
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DOI: https://doi.org/10.1007/s10038-004-0194-8
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