Summary
Seven kinds of DNA probes which recognize hypervariable loci were applied for paternity test. The putative father was decreased and unavailable for the test. The two legitimate children and their mother (the deceased's wife) and the four illegitimate children and their mother (the deceased's kept mistress) were available for analysis. Paternity index of four illegitimate child was investigated. Allelic frequencies and their confidence intervals among unrelated Japanese individuals were previously reported from our laboratory, and co-dominant segregation of the polymorphism was confirmed in family studies. Cumulative paternity indices of four illegitimate children from 16 kinds of standard blood group markers were 165, 42, 0.09, and 36, respectively. On the other hand, cumulative paternity indices from 7 kinds of DNA probes are 2,363, 4,685, 57,678, and 54,994, respectively, which are 14, 113, 640, 864, and 1,509 times higher than that from standard blood group markers. The DNA analyses gave nearly conclusive evidence that the putative father was the biological father of the children. Especially, the paternity relation of the third illegitimate child could not be established without the DNA analyses. Accordingly, DNA polymorphism is considered to be informative enough for paternity test.
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Allen, R.W., Bliss, B. and Pearson, A. 1989. Characteristics of a DNA probe (pa3′HVR) when used for paternity testing.Transfusion 29:477–485.
Baird, M., Balazs, I., Giusti, A., Miyazaki, L., Nicholas, L., Wexler, K., Kanter, E., Glassberg, J., Allen, F., Rubinstein, P. and Sussmann, L. 1986. Allele frequency distribution of two highly polymorphic DNA sequences in three ethnic groups and its application to the determination of paternity.Am. J. Hum. Genet. 39:489–501
Balazs, I., Baird, M., Clyne, M. and Maeda, E. 1989. Human population genetic studies of five hypervariable DNA loci.Am. J. Hum. Genet. 44:182–190.
Boonlayangoor, P.W. 1989. True paternity or exclusion: Analysis in the case of a deceased party.J. Forens. Sci. 34:703–707.
Capon, D.J., Chen, E.Y., Levinson, A.D., Seeburg, P.H. and Goeddel, D.V. 1983. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.Nature 302:33–37.
Garber, R.A. and Morris, J.W. 1983. General equations for the average power of exclusion for genetic systems of n codominant alleles in one-parent and no-parent cases of desputed parentage. InInclusion Probabilities in Parentage Testing, Walker, R.H., ed., American Association of Blood Banks, Arlington, Virginia, pp. 277–280.
Gendler, S.J., Burchell, J.M., Duhig, T., Lamport, D., White R., Parker, M. and Taylor-Paradimitriou, J. 1987. Cloning of partial cDNA encoding differentiation and tumor-associated mucin glycoproteins expressed by human mammary epithelium.Proc. Natl. Acad. Sci. U.S.A. 84: 6060–6064.
Gill, P., Jeffreys, A.J. and Werrett, D.J. 1985. Forensic application of DNA ‘fingerprints’,Nature 318:577–579
Giusti, A., Baird, M., Pasquale, S., Balazs, I. and Glassberg, J. 1986. Application nucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm.J. Forens. Sci. 31:409–417.
Higgs, R.D., Goodbourn, S.E.Y., Wainscoat, J.S., Clegg, J.B. and Weatherall, D.J. 1981. Highly variable regions of DNA flank the human alpha globin genes.Nucleic Acids Res. 9:4213–4224.
Ito, H., Yasuda, N. and Matsumoto, H. 1985. The probability of parentage exclusion based on restriction fragment length polymorphisms.Jpn. J. Human Genet 30:261–269.
Jeffreys, A.J., Wilson, V. and Thein, S.L. 1985a. Hypervariable ‘minisatellite’ regions in human DNA.Nature 314:67–73.
Jeffreys, A.J., Brookfield, J.E.Y. and Semenoff, R. 1985b. Positive identification of an immigration test-case using human DNA fingerprints.Nature 317:818–819.
Jeffreys, A.J., Royle, N.J., Wilson, V. and Wong, Z. 1988. Spontaneous mutation rates to new length alleles at tandem repetitive hypervariable loci in human DNA.Nature 332:278–281.
Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982.Molecular Cloning A laboratory manual. Cold Spring Harbor Lab., Cold Spring Harbor, New York.
Nakamura, Y., Leppert, M., O'Connell, P., Woff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumlin, E. and White, R. 1987. Variable number of tandem repeat (VNTR) markers for human gene mapping.Science 235:1616–1622.
nakamura, Y., Lathrop, M., O'Connell, P., Leppert, M., Barker, D., Wright, E. Skolnick, M., Kondoleon, S., Litt, M., Lalouel, J.M. and White, R. 1988a. A mapped set of DNA marker for human chromosome 17.Genomics 2:302–309.
Nakamura, Y., Lathrop, M., O'Connell, P., Leppert, M., Lalouel, J.M. and White, R. 1988b. A mapped set of DNA markers for human chromosome 15.Genomics 3:342–346.
Odelberg, S.J., Demers, D.B., Westin, E.H. and Hossaini, A.A. 1988. Establishing paternity using minisatellite DNA probes when the putative father is unavailable for testing.J. Forens. Sci. 33:921–928.
Odelberg, S.J., Plaetke, R., Eldridge, J.R., Ballard, L., O'Connell, P., Nakamura, Y., Leppert, M., Lalouel, J.M. and White, R. 1989. Characterization of eight VNTR loci by agarose gel electrophoresis.Genomics 5:915–924.
Wolff, R.K., Nakamura, Y. and White, R. 1988. Molecular characterization of a spontaneously generated new allele at a VNTR locus: No exchange of flanking DNA sequence.Genomics 3:347–351
Yokoi, T., Nata, M., Odaira, T. and Sagisaka, K. 1990a. Hypervariable polymorphic VNTR loci for parentage testing and individual identification.Jpn. J. Human Genet. 35:179–188.
Yokoi, T., Nata, M., Odaira, T. and Sagisaka, K. 1990b. Hypervariable regions of DNA for parentage testing and individual identification.Z. Rechtsmed. 103:487–497.
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Yokoi, T., Odaira, T., Nata, M. et al. Investigation of paternity establishing without the putative father using hypervariable DNA probes. Jap J Human Genet 35, 235–244 (1990). https://doi.org/10.1007/BF01876852
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DOI: https://doi.org/10.1007/BF01876852