Abstract
Mud volcanoes are geological structures in the oceans that have key roles in the functioning of the global ecosystem. Information on the dynamics of benthic viruses and their interactions with prokaryotes in mud volcano ecosystems is still completely lacking. We investigated the impact of viral infection on the mortality and assemblage structure of benthic prokaryotes of five mud volcanoes in the Mediterranean Sea. Mud volcano sediments promote high rates of viral production (1.65–7.89 × 109 viruses g−1 d−1), viral-induced prokaryotic mortality (VIPM) (33% cells killed per day) and heterotrophic prokaryotic production (3.0–8.3 μgC g−1 d−1) when compared with sediments outside the mud volcano area. The viral shunt (that is, the microbial biomass converted into dissolved organic matter as a result of viral infection, and thus diverted away from higher trophic levels) provides 49 mgC m−2 d−1, thus fuelling the metabolism of uninfected prokaryotes and contributing to the total C budget. Bacteria are the dominant components of prokaryotic assemblages in surface sediments of mud volcanoes, whereas archaea dominate the subsurface sediment layers. Multivariate multiple regression analyses show that prokaryotic assemblage composition is not only dependant on the geochemical features and processes of mud volcano ecosystems but also on synergistic interactions between bottom-up (that is, trophic resources) and top-down (that is, VIPM) controlling factors. Overall, these findings highlight the significant role of the viral shunt in sustaining the metabolism of prokaryotes and shaping their assemblage structure in mud volcano sediments, and they provide new clues for our understanding of the functioning of cold-seep ecosystems.
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Introduction
Submarine mud volcanoes are geological structures that are formed by the eruption of mud, fluids and gases (typically methane) from deep-subsurface sediment reservoirs (Milkov, 2000). Global estimates suggest that 103–105 mud volcanoes are distributed worldwide, mostly concentrated on the continental margins and abyssal plains (Dimitrov, 2002). Mud volcanoes are expected to have key roles in the carbon budget, biogeochemical cycles, climate regulation, biomass production and maintenance of biodiversity, thus providing essential ‘goods and services’ for global ecosystems (Niemann et al., 2006; Jørgensen and Boetius, 2007).
The emission of methane and other hydrocarbons, the presence of gas hydrates, and the release of high concentrations of sulphides sustain an enormous microbial biomass (Pimenov et al., 1997; Boetius and Suess, 2004; Joye et al., 2004). At present, we know that microbial assemblages in cold-seep ecosystems are typically dominated by sulphate-reducing bacteria and methanotrophic archaea, which mediate anaerobic oxidation of methane with sulphate (Jørgensen and Boetius, 2007). This process represents one of the major global sinks for methane (Michaelis et al., 2002; Niemann et al., 2006; Pachiadaki et al., 2010).
Although viruses dominate all of the ecosystems on the Earth numerically (outnumbering prokaryotes by about 10-fold; Suttle, 2005; Danovaro et al., 2008a) and they are a major cause of mortality for a wide range of marine organisms (Fuhrman, 1999; Suttle, 2005), their role in ecosystems characterised by seepage is still unclear. Information available from both shallow-water hydrothermal vents (Mediterranean Sea and Pacific Oceans; Manini et al., 2008) and cold seeps (Sagamy Bay, Japan, and Gulf of Mexico; Middelboe et al., 2006; Kellogg, 2010; respectively) suggests that these environments can be either ‘hot-spots’ or ‘cold-spots’ of viral activity (that is, enhanced or reduced viral activity at seep sites, as compared with surrounding sediments). However, information on the dynamics of benthic viruses and their interactions with prokaryotes in mud volcano ecosystems is still completely lacking.
Recent studies carried out in marine sediments worldwide have indicated that the viral shunt, which was originally described for pelagic systems (Wilhelm and Suttle, 1999), is also an essential source of organic detritus in benthic ecosystems. Here, viral lysis of the infected microbes transforms the cell contents and biomass into organic detritus, which contribute greatly to the metabolism of non-infected prokaryotes (Danovaro et al., 2008b).
Here, we report the results of the first investigation conducted on viral production and decay, viral impact on prokaryotic mortality, and the contribution of the viral shunt to C cycling in five mud volcanoes of the Mediterranean Sea. We also explore the role of viral infection in controlling prokaryotic assemblage structure in these ecosystems.
Methods
Study site and sampling strategy
Sediment samples were collected in the Sicily Channel (Western Mediterranean; Figure 1) from February to July, 2007. The region under investigation has about 175 mud volcanoes and a venting area of about 0.1 km2 (Holland et al., 2003). The sampling strategy included a set of seven sampling areas, five of which were located in mud volcanoes (MV02, MV03, MV04, MV05 and MV06), with two areas located along the slope at similar depths and without gas emissions (hereafter defined as the controls: CTRL1 and CTRL2; Figure 1). The geophysical survey led to the defining of two main geomorphological structures: (i) terrace-like carbonate structures (hereafter referred to as ridges), which consist of large tabular sub-elongated structures that are elevated up to 5 m to 10 m from the sea floor, and which have flat tops that are surmounted by several carbonate structures; and (ii) dome-shaped structures that are isolated or in clusters and are 50 m to 200 m wide and up to 5 m high. A detailed description of mud volcano areas was reported by Savini et al. (2009). Seawater samples were collected within 5 m of the seabed at the MV04, MV05 and MV06 stations, using Niskin bottles. The gas (methane) dissolved in these samples was analysed by gas chromatography (Carle AGC 100–400 TCD-FID GC) at Isotech Labs Inc. (Champaign, IL, USA). At each site, three undisturbed sediment cores were collected using a NIOZ-type box-corer, which allows collection of samples that can be hermetically closed. Immediately after sampling, sediment sub-aliquots were collected for analysis of the biochemical composition of the organic matter and for microbiological parameters. For analyses of the biochemical composition of the organic matter in the sediments (in terms of protein, carbohydrate, lipid and total phytopigment), the samples were stored at −20 °C.
To provide accurate estimates of viral abundance, production and decay, the surface (0–1 cm) and sub-surface (10–15 cm) layers from each sediment core were immediately processed onboard, without the addition of any preservative (Danovaro et al., 2001; Dell’Anno et al., 2009). This was because it is widely recognised that the use of formaldehyde or glutaraldehyde for sample storage can result in a rapid loss of viruses in sediment samples (Helton et al., 2006; Dell’Anno et al., 2009).
For the prokaryotic counts, samples from these surface (0–1 cm) and sub-surface (10–15 cm) layers of each sediment core were fixed with buffered 2% formalin and stored at 4 °C until processed (within two weeks; Danovaro et al., 2002). For analysis of prokaryotic heterotrophic C production, additional subsamples from the top first 1 cm were processed immediately after retrieval, as described below. For analysis of the prokaryotic assemblage structure by catalysed reporter deposition–fluorescence in situ hybridisation (CARD-FISH), the surface (top 1 cm) and subsurface (10–15 cm layer) sediment aliquots of each core were fixed with 2% paraformaldehyde (polymerised formaldehyde, dry chemical), washed with phosphate-buffered saline solution (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.6), and then stored in phosphate-buffered saline solution: 96% ethanol (1:1) at −20 °C until further processing (Pernthaler et al., 2004).
Biochemical composition of organic matter
The concentrations of proteins, carbohydrates and lipids in the sediment were determined spectrophotometrically, according to Danovaro (2010), and expressed as bovine serum albumin, glucose and tripalmitine equivalents, respectively. The sum of the carbohydrate, protein and lipid concentrations converted into carbon equivalents (using the conversion factors of 0.40, 0.49 and 0.75 μg C μg−1, respectively) was defined as the biopolymeric organic carbon (Pusceddu et al., 2009). Chlorophyll-a and phaeopigment concentrations were determined fluorometrically, according to Danovaro (2010). Total phytopigments were defined as the sum of chlorophyll-a and phaeopigments, and their concentrations are reported as μg g−1. Details of the analyses of the biochemical composition of the organic matter are reported in the Supplementary Materials.
Viral abundance
Viral counting was carried out as described by Danovaro et al. (2001). Sediment sub-samples (0.5 cm3 of wet sediment) were diluted in an equal volume of virus-free seawater (filtered at 0.02 μm pore size using disposable syringe filters; Anotop 25, Whatman; dilution 1:1, v:v), and treated with pyrophosphate (final concentration, 5 mM) and ultrasound (three times, for 1 min each; Branson 2200 sonicator, 60 W) to increase the extraction yields. The samples were then diluted 100–500-fold in virus-free water and filtered onto 0.02 μm pore size filters (Anodisc Al2O3; 25 mm diameter, Whatman). Then the filters were stained using SYBR Green I (10 000 × in anhydrous dimethyl sulphoxide; Molecular Probes-Invitrogen, Grand Island, NY, USA), incubated in the dark for 20 min, and then mounted on glass slides with a drop of 50% phosphate buffer (6.7 mM, pH 7.8) and 50% glycerol containing 0.5% ascorbic acid (Noble and Fuhrman, 1998). Viral counting was performed under epifluorescence microscopy (Zeiss Axioskop 2; magnification, × 1000; filter set #9, excitation BP 450–490, beam splitter FT 515, emission LP 520), by examining at least 10 fields per slide, and counting at least 400 viral particles per filter. The viral abundance was expressed as viruses per g dry sediment (after desiccation at 60 °C for 24 h).
Prokaryotic abundance
For prokaryotic counting, ca. 1 cm3 of sediment from the top 1 cm and from the 10–15 cm layer of each sediment core were analysed as previously described by Danovaro et al. (2002). Three replicate sediment sub-samples were each suspended in tetrasodium pyrophosphate (final concentration, 5 mM) and sonicated three times (Branson Sonifier 2200; 60 W for 1 min). The samples were then diluted with sterile seawater that was pre-filtered through 0.2-μm-pore-size filters (25 mm diameter disposable syringe filters; Whatman). Following filtering onto 0.2 μm pore size filters (Anodisc Al2O3, 25 mm diameter; Whatman), the samples were stained using SYBR Green I (10 000 × concentrate in anhydrous dimethyl sulphoxide; Molecular Probes-Invitrogen), and the filters were incubated in the dark for 20 min and mounted on microscope slides with 20 μl 50% phosphate buffer (pH 7.8) and 50% glycerol containing 0.5% ascorbic acid. Total prokaryotic counts were obtained under epifluorescence microscopy (Zeiss Axioskop 2, magnification × 1000, filter set #9, excitation BP 450–490, beam splitter FT 515, emission LP 520) by examining at least 10 fields per slide, to count at least 200 cells per filter. The data were normalised to sediment dry weight after desiccation (60 °C, 24 h).
Viral production and decay
Viral production in the sediments was determined as described by Dell’Anno et al. (2009). Three replicate sediment samples of about 25 ml were transferred into sterile tubes and mixed with 25 ml virus-free seawater. Subsamples (0.5 ml) were collected for viral counts at 0, 1.5, 3, 6 and 12 h. As the increases in viral abundance were not linear over the first 12 h of incubation, in all of the samples, the net viral production was determined as the maximum increment of viral abundance per g dry sediment per h.
Viral decay was defined as the rate of disappearance of free viruses (Danovaro et al., 2008a), and this was determined following the same procedure used for the analysis of viral production, by monitoring the decrease of viruses over time according to Corinaldesi et al. (2010). Sediment subsamples (1:1, sediment:virus-free seawater) were collected at 0, 1.5, 3, 6, 12 and 24 h. The measurements of viral decay were started only when a significant decrease in the viral abundance was evident, that is, generally 6–12 h after the beginning of the experiment. Viral decay rates were estimated as the maximum rate of decrease of the viral abundance during the time-course experiments. The viral decay rate was expressed as the number of viruses per g dry sediment per h. Gross viral production was estimated as the sum of the net viral production and decay rates.
Prokaryotic heterotrophic production
Prokaryotic heterotrophic production was measured using [3H]-leucine incorporation, following the procedure previously described by Danovaro et al. (2002). Sediment sub-samples (200 μl) were supplemented with an aqueous solution of [3H]-leucine (0.2 nmol final concentration, specific activity, 72 Ci mmol−1), and incubated for 1 h in the dark at the in situ temperature. Three sediment blanks were run in parallel, adding ethanol immediately before the [3H]-leucine addition. After the incubation, the samples were supplemented with ethanol (80%) and stored at 4 °C until they were processed in the laboratory. Briefly, the sediments were washed two times with ethanol (80%) by mixing, centrifuging at 14 000 g for 5 min, and discarding the supernatant. The sediment was then re-suspended in 80% ethanol and transferred onto a polycarbonate membrane filter (0.2 μm pore size, Nucleopore, 25 mm diameter) using a filtration apparatus and a vaccum pump (Millipore, Billerica, MA, USA, <100 mm Hg). Subsequently, each filter was washed four times with 2 ml 5% trichloroacetic acid, and then each filter was transferred into a pyrex test-tube containing 2 ml 2 M NaOH and heated to 100 °C for 2 h. After centrifugation at 800 g to separate the sediment residue, 1 ml supernatant was transferred to scintillation vials containing an appropriate scintillation fluid. The incorporated radioactivity (as disintegration per minute) in the sediment samples was measured by determining the counts per minute in a liquid scintillation counter (Packard Tri-Carb 300). The data were normalised to sediment dry weight after desiccation (60 °C, 24 h).
Burst size and VIPM
Prokaryotic burst size (the number of viruses released by cell lysis due to viral infection) was calculated as the ratio of viral production to cells killed (Danovaro et al., 2008b). The number of prokaryotes killed by the viruses was estimated by time-course experiments on the basis of the expected number of prokaryotes produced (calculated as prokaryotic turnover multiplied by prokaryotic abundance) and the number of prokaryotes actually counted over the same time interval (Corinaldesi et al., 2007).
VIPM was calculated as follows:
where: KP is the number of prokaryotes killed by the viruses per g sediment per h, and TP is the total prokaryotic abundance per g sediment.
Catalysed reporter deposition–fluorescence in-situ hybridisation
The analysis of the benthic prokaryotic assemblage structure at the domain level (Bacteria vs Archaea) as performed by CARD-FISH, as previously described (Teira et al., 2004; Schippers et al., 2005; Danovaro et al., 2009). Methodological details are reported in the Supplementary Materials.
Statistical analyses
To test for differences in all of the variables investigated between the sampling sites, one-way analysis of variance was applied using the GMAV 5.0. software (University of Sidney, Australia), after testing for the homogeneity of variances using the Cochran test. When significant differences were encountered, Tukey's post-hoc comparison tests (at α=0.05) were also carried out, to determine in which station(s) the parameters investigated were significantly different.
Multivariate multiple regression analysis was also used for investigation of the biotic and abiotic factors that influence viral distribution and prokaryotic assemblage composition. All of the analyses were carried out with routine distance-based multivariate analysis for a linear model forward (Anderson, 2004). The P-values were obtained using 4999 permutations of residuals under the reduced model (Anderson, 2001).
Results
Methane concentrations and biochemical composition of organic matter
The methane, biopolymeric C, and phytopigment concentrations are reported in Table 1. Both the domes and ridges are influenced by methane seeps, with the highest concentrations of methane dissolved in the seawater at the MV05 station.
Biopolymeric C concentrations (used as a proxy for the available trophic resources; Pusceddu et al., 2009) in the surface sediments ranged from 0.27±0.04 to 3.25±1.15 mgC g−1 (at stations MV06 and CTRL2, respectively), while the total phytopigment concentrations varied from 3.8±0.8 μg g−1 to 33.4±10.5 μg g−1 (at stations CTRL2 and MV02, respectively).
Viral and prokaryotic abundance
Viral abundance in the surface sediments (0–1 cm layer) ranged from 5.42±1.53 × 108 to 31.87±1.24 × 108viruses g−1 (at stations CTRL1 and MV05, respectively; Figure 2a), and it was significantly higher in the mud volcanoes than in the control stations (analysis of variance, P<0.01). At all of the stations, the viral abundance decreased significantly in the subsurface sediment layers (range, 3.63±0.61 × 108 to 9.85±1.28 × 108 viruses g−1, at the 10–15 cm sediment depth at stations MV06 and MV02, respectively, P<0.05, Figure 2a).
Prokaryotic abundance in the surface sediments (0–1 cm layer; Figure 2b) ranged from 1.89±0.30 × 108 cells g−1 to 11.31±2.01 × 108 cells g−1 (at stations CTRL1 and MV02, respectively), and on average, it was significantly higher for the mud volcanoes than for the control stations (7.37 × 108 vs 3.02 × 108 cells g−1, P<0.01).
In the subsurface sediments (10–15 cm layer), the prokaryotic abundance (range, 0.60±0.14–5.22±0.65 × 108 cell g−1 at stations MV06 and MV02, respectively) was significantly lower than in the surface sediments (P<0.05). The virus to prokaryote abundance ratio ranged from 1.6 to 7.9 in the surface sediments (at stations MV02 and MV05, respectively), and from 1.9 to 10.6 in the subsurface sediments (at stations MV02 and MV05, respectively; Figure 2c).
Viral production and decay, and VIPM
The net viral production in the surface sediments ranged from 0.25±0.02 × 108 to 3.29±0.43 × 108 virus g−1 h−1 (at stations CTRL1 and MV03, respectively). Significant differences were observed among the stations, with viral production higher in the mud volcano sediments than in the control sediments (P<0.05; Figure 3a).
Viral decay ranged from 0.37±0.09 × 107 to 8.83±2.91 × 107 viruses g−1 h−1 (at stations CTRL1 and MV04, respectively; Figure 3b). The mud volcano sediments were characterised by viral decay rates (on average, 5.25 × 107 viruses g−1 h−1) that were significantly higher than in the control sediments (on average, 0.87 × 107 viruses g−1 h−1; P<0.01). Viral decay accounted for 22% of the gross production in the mud volcano sediments, and 25% in the control sediments.
VIPM in the mud volcano stations, except for MV02, was significantly higher than in the control stations (P<0.01; on average, 33% vs 23% killed prokaryotes per day in mud volcano and control sediments, respectively; Figure 3c).
Prokaryotic heterotrophic C production and biomass
Prokaryotic heterotrophic C production in surface sediments ranged from 43.0±12.4 to 346.6±54.9 ngC g−1 h−1 (at stations CTRL1 and MV03, respectively) and was ca. 6-fold higher in the mud volcano sediments than in the control sediments (Figure 4a). The prokaryotic biomass in the surface sediments ranged from 4.5±0.7 to 33.5±8.6 μgC g−1 (at stations CTRL1 and MV02, respectively), and on average, for the mud volcanoes it was ca. three-fold higher than in the control sediments (21.6 vs 6.5 μgC g−1, respectively; Figure 4b). The turnover rates of prokaryotic biomass ranged from 0.18 d−1 to 0.33 d−1 (at stations MV02 and MV03, respectively), and on average, they were similar between the mud volcanoes and the control sediments (0.28 vs 0.25 d−1, respectively; see Supplementary Figure S1).
Prokaryotic assemblage structure
The number of benthic prokaryotes detected using CARD-FISH (that is, the total bacterial abundance plus the total archaeal abundance) accounted for, on average, 73% of the total prokaryotic abundance as determined using SYBR Green I.
The abundance of bacteria and archaea was significantly higher in the surface sediments of the mud volcanoes than in the control sediments (on average, 4.65 × 108 vs 1.16 × 108 cells g−1 for bacteria, and 1.13 × 108 vs 0.54 × 108 cells g−1 for archaea; P<0.01; Figure 5a). The abundance of Archaea increased with the depth of the sediment, and was ca. double the abundance of bacteria (on average, 6.37 × 107 vs 3.38 × 107 cells g−1, respectively; Figure 5b).
Within the archaeal domain, the contributions of crenarchaea and euryarchaea were similar in surface sediments of mud volcanoes (on average, ca. 10% of the total cell counts by CARD-FISH; see Supplementary Figure S2a). Conversely, the abundance of crenarchaea was ca. three-fold higher than that of euryarchaea in the control sediments (on average, 23.3% vs 8.3%).
Crenarchaea accounted for a significantly lower fraction than for euryarchaea in the subsurface sediments of the mud volcanoes (on average, 3.3% vs 32.1%; Figure S2b).
Discussion
The impact of viruses on prokaryotic activity
Viral abundance in mud volcano sediments was significantly higher than that determined in the control sediments, with values similar to that previously reported in cold seeps of the Gulf of Mexico (Kellogg, 2010), although much higher than that observed in a cold seep of Sagami Bay (Japan, Middelboe et al., 2006).
The determination of viral and prokaryotic abundance alone does not provide information on the dynamics of benthic viruses and their effects on prokaryotic assemblages (Danovaro et al., 2008b). We thus also investigated viral production, decay and impact on prokaryotes. Viral production in the mud volcano sediments was high and comparable to values reported for highly productive benthic coastal ecosystems (Danovaro et al., 2008a). In addition, viral production in the mud volcanoes investigated was significantly higher (up to one order of magnitude) than that of the control sediments. Recent studies have indicated that lysogenic infection can prevail on the lytic strategy both in shallow and deep hydrothermal vent ecosystems (Williamson et al., 2008; Maugeri et al., 2010). However, our results suggest that the environmental conditions of mud volcanoes promote high rates of viral replication through lytic infection, which indicates that these ecosystems represent hot spots of viral activity.
Viral production co-varied with the metabolic activity of the prokaryotes (prokaryotic C production: n=15, r=0.89, P<0.01), which was, on average, ca. six-fold higher in the mud volcano sediments than in the control sediments. The high heterotrophic prokaryotic C production in mud volcano sediments was associated with high concentrations of organic C derived from primary production processes (that is, the phytopigment content was ca. six-fold higher than in the control sediments).
The viral impact on prokaryotes in the mud volcano sediments was, on average, higher than in the control sediments (33% and 23% of prokaryotes killed per day, respectively), and was even higher when compared with prokaryotic mortality previously reported for marine sediments at similar depths worldwide (Danovaro et al., 2008b). This suggests that viral infection has crucial implications in the functioning of mud volcano ecosystems. We also found that viral and prokaryotic abundance in subsurface sediments (the 10–15 cm layer) was significantly lower than in the surface sediments (the 0–1 cm layer). However, viral abundance decreased with depth in the sediment to a lesser extent than prokaryotic abundance. Consequently, the virus-to-prokaryote abundance ratios in the subsurface sediments were up to 3-fold higher than in surface sediments. This pattern allows us to hypothesise that the viral impact on their hosts in these subsurface sediments is even greater than in the surface sediments (Corinaldesi et al., 2010). Overall, these findings suggest that the environmental conditions in mud volcanoes sustain high prokaryotic heterotrophic metabolism, and promote highly dynamic virus–prokaryote interactions.
Factors influencing the viral shunt in mud volcano sediments
Viral abundance in marine sediments is generally related to viral production (Danovaro et al., 2008a), although this was not the case for the present study, as the patterns of viral abundance in the mud volcano sediments were opposite to those of the viral production. In particular, the highest viral abundance and the lowest viral production rates were observed in the mud volcano sediments that were characterised by the highest gas emission (MV05, viral abundance one third of that in the other mud volcano stations). The uncoupled patterns between viral abundance and production can be dependant upon viral decay rates (Corinaldesi et al., 2010). Indeed, at the MV05 station, we found the lowest viral decay rate and contribution to the gross viral production (12%).
Statistical multivariate analyses reveal also that ca. 95% of the total variance of the viral abundance was explained by the biopolymeric carbon and methane concentrations, which can influence the viral distribution by stimulation of the metabolism of their host and/or by supplying mud volcano sediments with viruses not produced in situ.
We conclude here that the main factors driving the highly dynamic viral assemblages in mud volcanoes are different and more complex than those in sediments without seepage.
The viral shunt fuels the metabolism of benthic prokaryotes and influences their assemblage structure
Viral lysis of prokaryotic cells results in the release of cellular detritus, which can be an important organic source for prokaryotic metabolism (Corinaldesi et al., 2007; Suttle, 2007; Danovaro et al., 2008a, 2008b). In mud volcano sediments, we estimated that the C released by viral lysis was, on average, 47 mg C m−2 d−1. This is ca. four-fold higher than that released in the control sediments, which in turn is similar to values reported for other benthic systems at similar depths worldwide (Danovaro et al., 2008a, 2008b). If we also include the organic C released by viral decay in the C flux derived from viral-shunt processes, the amount of C derived by viral lysis in the mud volcano sediments was, on average, 49 mg C m−2 d−1. Assuming a conservative value of C conversion efficiency in prokaryotic biomass of 20% (del Giorgio and Cole, 1998), the amount of C released by viral lysis accounts for ca. 21% of the prokaryotic C demand in the mud volcano sediments. Such values are comparable to those previously reported in different marine pelagic systems (5–30% of the prokaryotic carbon demand; Wilhelm and Suttle, 1999; Bonilla-Findji et al., 2008).
Recent studies have suggested that viral lysis of microorganisms is also a source of recalcitrant-dissolved organic carbon, and as this is resistant to microbial degradation, it can persist in the ocean interior for thousands of years (Jiao et al., 2010).
Overall, these findings suggest that the total C released by viral lysis in mud volcano sediments can sustain prokaryotic assemblages through stimulation of the metabolism of non-infected prokaryotes, and it can represent a carbon source that fuels the dissolved organic carbon pool of the overlying water column.
Information on the impact of viral infection on prokaryotic biodiversity and community structure in benthic ecosystems is still largely lacking (Hewson and Fuhrman, 2007). In the present study, we found that bacteria are the dominant component of prokaryotic assemblages both in surface sediments of mud volcanoes and in the controls. Conversely, in the subsurface sediments of mud volcanoes, archaea account for ca. 60% of prokaryotic abundance, on average. The increase in the relative importance of archaea with increasing depth in sediments has already been reported for continental margins (Biddle et al., 2006; Lipp et al., 2008) and other mud volcano ecosystems (Lösekann et al., 2007), which suggests that this component is more adapted than bacteria to live and grow under the environmental conditions occurring in the mud volcano subsurface.
Previous studies have suggested that crenarchaea typically dominate over euryarchaea in oxygenated deep waters (De Long et al., 1999; Karner et al., 2001) and surface sediments (Vetriani et al., 1999; Danovaro et al., 2009). We found the same pattern in the control sediments, whereas euryarchaea were dominant in the surface and subsurface sediments of the mud volcanoes. The dominance of euryarchaea over crenarchaea has been previously reported for gas-hydrate-bearing and methane-bearing sediments, and this was related to the exploitation of the methane released by the seeps (Inagaki et al., 2006; Parkes et al., 2007; Pachiadaki et al., 2010).
To determine which factors mainly influence the composition of prokaryotic assemblages in mud volcano sediments, we carried out multivariate multiple regression analysis. We found that in all of the systems, >80% of the total variance of the prokaryotic assemblage composition (as ratios between bacteria and archaea and crenarchaea and euryarchaea) was explained by the availability of trophic resources (in term of phytopigments, biopolymeric C and C released by the viral shunt) and by the ‘viral predatory pressure’ (that is, VIPM).
These findings suggest that the structure of the prokaryotic assemblages that inhabit these mud volcanoes is not only dependant on the geochemical features and processes of mud volcano ecosystems but also on synergic interactions with both bottom-up (that is, available trophic resources for prokaryotic assemblages, including phytopigments) and top-down (that is, viral-mediated prokaryotic mortality) controlling factors.
Overall, our results highlight the significant roles of viral infection in mud volcano sediments for sustaining the metabolism of prokaryotes and for the shaping of their assemblage structure, and they provide new clues towards an understanding of cold-seep ecosystem functioning.
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Acknowledgements
This study was supported by the EU funded project HERMIONE and by the National Projects MESC (COFIN PRIN 2005) and EXPLODIVE (FIRB 2008, contract no. I31J10000060001). We would like to thank Dr M Magagnini for support in the field work and laboratory analyses.
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Corinaldesi, C., Dell'Anno, A. & Danovaro, R. Viral infections stimulate the metabolism and shape prokaryotic assemblages in submarine mud volcanoes. ISME J 6, 1250–1259 (2012). https://doi.org/10.1038/ismej.2011.185
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DOI: https://doi.org/10.1038/ismej.2011.185
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