Abstract
Rye secalins were analyzed in the progeny of three test-crosses by sodium dodecyl sulphate Polyacrylamide gel electrophoresis (SDS-PAGE) using unreduced and reduced extracts of total seed proteins. Segregation for two ω-secalin bands and two 40K γ-secalin bands was studied in the three crosses. Both kinds of proteins showed a very tight linkage (0·25 ± 0·25 per cent recombination), finding one recombinant in 394 seeds. Segregation for two high molecular weight (HMW) secalin bands was analyzed in one test-cross. The data indicated linkage (36·03 ± 4·12 per cent recombination) between the structural genes for ω and 40K γ secalins (Sec 1) and for HMW secalins (Sec 3).
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Carrillo, J., Vazquez, J. & Orellana, J. Linkage relationships between the loci Sec 1 and Sec 3 in rye (Secale cereale L.). Heredity 64, 125–130 (1990). https://doi.org/10.1038/hdy.1990.16
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DOI: https://doi.org/10.1038/hdy.1990.16
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