Studies on the HAEC (human amniotic epithelial cell), a kind of stem cells, have suggested that it could be an excellent resource for cell replacement in the clinical practice, once differentiated into functional cells. In 2004-2005, transplantation of HAECs derived insulin secreting cells, induced by the nicotinamide and N2 in vitro, into streptozotocin-induce diabetic mice normalized the hyperglycemia. However, these cells only sustained the normal blood glucose for only tow months and could express both insulin and glucagon genes according to recent report, seemed that they were not stable and mature insulin secreting cells.

To get a better understanding of the transdifferentiation characteristics, we evaluated the chronological gene expression changes, such as PDX1, ngn3, pax4, pax6, nkx6.1, nkx2.2, insulin and glucose gene, by semi-quantity RT-PCR during a 14-day inductive course. Briefly, the cell samples were collected every other day and then semi-quantity RT-PCR was performed to examine the mRNA expression level. The immunocytofluorescental analysis were performed to detect the insulin or/and glucagon-positive cell number changes. The gene chronological expression analysis demonstrated a different expression trend between insulin and glucagon gene. Insulin gene's expression was first up-regulated in the first week and subsequently down-regulated, but the glucagon was sustained at a stable high level within the whole time course. Expression tendency of PDX1, pax4, nkx2.2 gene, known as important transcriptional factor for beta cell differentiation and insulin gene transcription, was similar to that of insulin gene. PDX1 was even negative in the last 4 days. Ngn3, nkx6.1 and pax6, which was important for alpha cell differentiation, were stable expressed in all of the samples except HAECs. Immunocytofluorescent analysis showed that double positive cells were the major part in the first week, after that, number of glucagons-positive cells increased step by step, whereas insulin-positive cells number decreased. Our results indicated that prevalent inductive method of HAECs-derived insulin secreting cells by addition of N2 and nicotinamide was not effective enough and many HAECs differentiated into glucagon secreting cells.