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Factors predicting allogeneic PBSCs yield after G-CSF treatment in healthy donors

Bone Marrow Transplantation volume 44, pages 613–615 (2009)Cite this article

G-CSF mobilized PBSCs are increasingly used in allo-SCT due to their relative ease of collection1 and because higher CD34+ cell doses may be associated with improved transplant outcomes in some settings.2, 3 However, some healthy donors may show poor mobilization response to G-CSF and poor subsequent CD34+ apheresis yields.4, 5 Therefore, identifying donors at risk for poor mobilization and a reliable estimate of a healthy donor's CD34+ cell mobilization response to G-CSF and subsequent CD34+ cells yield could be of value in optimizing transplantation approaches. The aim of this single center report was to analyze factors associated with the total CD34+ PBSC yield in an ethnically homogeneous Caucasian population of 95 healthy allogeneic adult donors.

All donors received G-CSF dosed at 10 μg/kg/day (Filgrastim, Amgen, France) for 5 days followed by leukapheresis. Complete blood counts (total WBC, Hb, Plts) were measured for all donors at baseline before G-CSF administration, before and after PBSC collection. Peripheral blood CD34+ cell counts were also measured before apheresis. Sixty-nine donors (73%) were healthy sibling donors, whereas 26 (27%) were healthy volunteer donors for HLA-identical unrelated transplants. All donors were undergoing their first PBSC mobilization (study period 2004–2008). Donors' demographic characteristics are summarized in Table 1. Briefly, the median (range) age, weight and height were 47 (18–81) years, 69 (43–106) kg and 170 (150–187) cm, respectively. The median body mass index (BMI) was 23.9 (15.2–35.7) kg/m2. As per the institutional policy, the targeted total number of CD34+ stem cells was between 4 and 8 × 106/kg recipient body weight to be collected in a maximum of three consecutive aphaeresis sessions while continuing G-CSF administration. Assessments were based on recipient body weight.

Table 1 Donors' demographics and post-G-CSF cell counts
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Authors and Affiliations

  1. Service d'Hématologie Clinique, CHU de Nantes, Nantes, France

    E Brissot, P Chevallier, T Guillaume, J Delaunay, S Ayari, V Dubruille, S Le Gouill, B Mahe, T Gastinne, N Blin, B Saulquin, P Moreau, J-L Harousseau & M Mohty

  2. Université de Nantes, Nantes, France

    S Le Gouill, P Moreau, J-L Harousseau & M Mohty

  3. INSERM U892, Nantes, France

    S Le Gouill, G Flandrois, A Devys, P Moreau, J-L Harousseau & M Mohty

  4. Etablissement Français du Sang, Nantes, France

    V Stocco, A Cesbron & F Dehaut

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  1. E Brissot
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  2. P Chevallier
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Correspondence to M Mohty.

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Brissot, E., Chevallier, P., Guillaume, T. et al. Factors predicting allogeneic PBSCs yield after G-CSF treatment in healthy donors. Bone Marrow Transplant 44, 613–615 (2009). https://doi.org/10.1038/bmt.2009.60

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