Abstract
The distribution of 3H-chlorambucil following its administration by subcutaneous injection to Yoshida ascites tumour-bearing rats has been examined, in an attempt to elucidate the metabolic fate and mode of action of this drug.
Drug uptake into the body tissue was rapid, with a high level of radioactivity being associated with the plasma and ascitic fluid during the initial 6-hour period after treatment. Previous studies in vitro had shown that chlorambucil-resistant cells accumulated less drug than their sensitive counterparts: this discrepancy was also observed after in vivo drug treatment and was reflected in the two-fold difference in extent of binding of tritium to DNA, RNA and protein isolated from the 2 cell strains. These results might in part explain the observed difference in metabolism of chlorambucil by the resistant and sensitive strain of Yoshida ascites sarcoma cells.
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Hill, B., Riches, P. The Absorption, Distribution and Excretion of 3H-Chlorambucil in Rats Bearing the Yoshida Ascites Sarcoma. Br J Cancer 25, 831–837 (1971). https://doi.org/10.1038/bjc.1971.96
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DOI: https://doi.org/10.1038/bjc.1971.96