Abstract
Aim:
Panax notoginseng is a cultivated ginseng species highly valued for its various pharmacological activities mostly associated with triterpenoid saponin glycosides. It would be of great interest to understand biodiversity in this ginseng species after its long history of domestication.
Methods:
We collected 92 random sampled 3-year-old P notoginseng plants from 4 counties of Wenshan prefecture in Yunnan province, China and documented their morphological features of plant height, stem color, number of leaves/leaflets and dry weight of tap root. Their genetic diversity was evaluated by fluorescent amplified fragment length polymorphism (fAFLP) analysis.
Results:
Among the samples collected, variable morphological features were observed. For these 4 populations (Zhulijie, Shangliuhe, Bazai and Jinbuhuan) analyzed by fAFLP, percentage of polymorphic bands among the total number of 582 discrete bands were 74.05%, 45.36%, 38.83% and 51.89% respectively. Mean genetic heterozygosity were 0.166, 0.093, 0.094 and 0.125. On the other hand, Nei genetic distances among populations were all <0.03. Further analysis of molecular variance (AMOVA) attributed most (93.5%) genetic diversity to within population variation. Principal coordinates analysis (PCA) did not group any population distinctively.
Conclusion:
This domesticated ginseng species still maintains a fair level of biodiversity and this conclusion is consistent with the local practice of non-selective collection of seeds for next season planting. There was no genetic drift in populations. Biodiversity of P notoginseng can be exploited to improve this important herb through breeding. Two possible strategies include inbreeding for pure lines and hybrid breeding with genetic divergent parents for hybrid vigor.
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This study was supported by an internal research grant of the Temasek Life Sciences Laboratory (Yan HONG, Deborah HONG), a grant from the US National Institutes of Health and National Center for Complementary and Alternative Medicines (Yan HONG, Planning Grant: 1 R21 AT001945-01), National Natural Science Fundation of China (Chong-Ren YANG and Dong WANG, Grant: 30472156), Academic Research Fund (R-148-000-056-112) and International Collaboration Fund of the National University of Singapore (Hwee-ling KOH and Dong WANG).
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Wang, D., Hong, D., Koh, Hl. et al. Biodiversity in cultivated Panax notoginseng populations. Acta Pharmacol Sin 29, 1137–1140 (2008). https://doi.org/10.1111/j.1745-7254.2008.00875.x
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DOI: https://doi.org/10.1111/j.1745-7254.2008.00875.x
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