Abstract
Aim:
To evaluate the phylogenetic relationships among related species of Lycium samples.
Methods:
Random amplified polymorphic DNA (RAPD) fingerprinting and lab-on-a-chip electrophoresis techniques were used to analyze the characteristics of Lycium species. Seven species and 3 varieties of Lycium were studied. Based on RAPD fingerprint data obtained from 11 primers, we proposed a new index, called dispersivity, using entropy theory and projection methods to depict the diversity of the DNA fingerprints.
Results:
Using the proposed dispersivity, primers were sorted and the dendrograms of the 7 species and 3 varieties of Lycium were constructed synthetically by merging primer information.
Conclusion:
Phylogenetic relationships among Lycium samples were constructed synthetically based on RAPD fingerprint data generated from 11 primers.
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Project supported by the National Natural Science Foundation of China (No 10371015 and 10329102) and a grant from the Hong Kong Baptist University (No FRG/02–03/II-62).
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Yin, Xl., Fang, Kt., Liang, Yz. et al. Assessing phylogenetic relationships of Lycium samples using RAPD and entropy theory. Acta Pharmacol Sin 26, 1217–1224 (2005). https://doi.org/10.1111/j.1745-7254.2005.00197.x
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DOI: https://doi.org/10.1111/j.1745-7254.2005.00197.x