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Inhibition of angiogenesis and tumor progression by hydrodynamic cotransfection of angiostatin K1-3, endostatin, and saxatilin genes

Cancer Gene Therapy volume 13, pages 563–571 (2006)Cite this article

Abstract

In vivo expression of angiostatin and endostatin, two different types of endothelial cell growth inhibitor, have been reported to inhibit vascularization in tumor tissues, resulting in tumor growth inhibition. Recently, in vivo expression of saxatilin, a novel disintegrin purified from snake (Gloydius saxatilis) venom, was able to strongly inhibit endothelial cell proliferation and smooth muscle cell migration, resulting in tumor growth inhibition. However, the antitumor efficacy of the individual antiangiogenic molecules expressed in vivo was not sufficiently potent to induce tumor regression in animal models. Therefore, in this study, we have systemically examined how combinational transfer of angiostatin, endostatin, and saxatilin genes affects neovascularization in tumor tissues and tumor progression in a mouse model. In Matrigel-implanted mice, cotransfection with plasmids encoding angiostatin K1-3 (pFLAG-Angio K1/3), endostatin (pFLAG-Endo), and saxatilin (pFLAG-Sax) resulted in the most effective inhibition of angiogenesis. In addition, hydrodynamic cotransfection of the three genes induced more inhibition of B16BL6 melanoma growth and pulmonary metastasis than other combinations of transfected genes. Compared with the empty vector-treated control group, cotreatment with the three plasmids reduced B16BL6 tumor growth by 89% and pulmonary metastasis by 90%. These results provide additional evidence supporting the combined systemic expression of antiangiogenic factors, such as angiostatin K1-3, endostatin, and saxatilin, as an alternative procedure for antiangiogenic cancer therapy.

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Acknowledgements

The authors wish to acknowledge the financial support of the National Cancer Center of Korea in the program year of 2005.

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science and Institute of Health Science, Yonsei University, Wonju, Republic of Korea

    K S Kim & Y S Park

  2. Department of Biochemistry, Yonsei University, Seoul, Republic of Korea

    D S Kim

  3. Cardiovascular Research Institute and BK21 projects for Medical Science, Yonsei University, College of Medicine, Seoul, Republic of Korea

    K H Chung

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  1. K S Kim
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Correspondence to Y S Park.

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Kim, K., Kim, D., Chung, K. et al. Inhibition of angiogenesis and tumor progression by hydrodynamic cotransfection of angiostatin K1-3, endostatin, and saxatilin genes. Cancer Gene Ther 13, 563–571 (2006). https://doi.org/10.1038/sj.cgt.7700924

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