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Novel immunotherapy for peritoneal dissemination of murine colon cancer with macrophage inflammatory protein-1β mediated by a tumor-specific vector, HVJ cationic liposomes

Cancer Gene Therapy volume 8, pages 852–860 (2001)Cite this article

Abstract

A critical issue for cancer treatment is control of metastatic or disseminated tumors. Although immune gene therapy has been considered as a possible strategy for treatment of such tumors, successful results have not yet been obtained. To evoke antitumor immunity more efficiently, macrophage inflammatory protein-1β (MIP-1β) was used for gene therapy of colon cancer in mice. Injection of hemagglutinating virus of Japan (HVJ) cationic liposomes–MIP-1β into subcutaneous tumor masses resulted in local expression of MIP-1β and local accumulation of CD4+ T lymphocytes. Few studies of cancer gene therapies have targeted peritoneal dissemination. In a mouse model of peritoneal dissemination of colon tumor, we used a luciferase-based assay to demonstrate that HVJ cationic liposomes had high tumor specificity and were effective vectors for transfer of genes in peritoneal dissemination. When mice were treated by intraperitoneal injection of HVJ cationic liposomes containing the MIP-1β gene, the survival periods of the MIP-1β–treated mice were significantly longer than those of control mice. Therefore, this HVJ cationic liposome strategy may serve as a powerful tool against peritoneal disseminated cancer. Cancer Gene Therapy (2001) 8, 852–860

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Acknowledgements

This work was supported by grants from the Ministry of Health, Labour, and Welfare, Japan.

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Authors and Affiliations

  1. Division of Gene Therapy Science, Osaka University School of Medicine, Suita, Osaka, Japan

    Toshio Miyata, Seiji Yamamoto, Kayo Sakamoto, Ryuichi Morishita & Yasufumi Kaneda

  2. First Department of Surgery, Kagoshima University School of Medicine, Sakuragaoka, Kagoshima, Japan

    Seiji Yamamoto

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  1. Toshio Miyata
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  2. Seiji Yamamoto
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  4. Ryuichi Morishita
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Correspondence to Yasufumi Kaneda.

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Miyata, T., Yamamoto, S., Sakamoto, K. et al. Novel immunotherapy for peritoneal dissemination of murine colon cancer with macrophage inflammatory protein-1β mediated by a tumor-specific vector, HVJ cationic liposomes. Cancer Gene Ther 8, 852–860 (2001). https://doi.org/10.1038/sj.cgt.7700384

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  • DOI: https://doi.org/10.1038/sj.cgt.7700384

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