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β-Adrenergic receptor gene polymorphisms and hemodynamic response to dobutamine during dobutamine stress echocardiography

The Pharmacogenomics Journal volume 8, pages 408–415 (2008)Cite this article

Abstract

Our objective was to determine if β1-adrenergic receptor (β1-AR) and β2-AR gene polymorphisms influence heart rate (HR), systolic blood pressure (SBP) and diastolic blood pressure (DBP) response to dobutamine during dobutamine stress echocardiography (DSE). Patients (n=163) undergoing clinically indicated DSE were enrolled. Dobutamine doses were titrated from 5 to 40 μg kg−1 min−1 at 3 min intervals and HR, SBP and DBP were measured. Genotypes were determined for β1-AR Ser49Gly, β1-AR Arg389Gly, β2-AR Arg16Gly and β2-AR Gln27Glu polymorphisms by polymerase chain reaction-restriction fragment length polymorphism analysis, pyrosequencing and single primer extension methods. β2-AR Glu27 homozygotes had a greater HR response at the highest dobutamine dose than Gln27 carriers (P=0.002). β2-AR Gly16 homozygotes had a lower HR response during 5–30 μg kg−1 min−1 of the dobutamine infusion protocol compared to Arg16 carriers (P=0.03). Differences in SBP by β2-AR codon 16 genotype and DBP by β1-AR codon 389 genotype were found at baseline and were maintained throughout DSE (P=0.06 and 0.02, respectively). However, the magnitude of SBP and DBP response to dobutamine did not differ significantly by β2-AR codon 16 or β1-AR codon 389 genotypes, respectively. These data suggest that the four selected β1- and β2-AR polymorphisms do not substantially influence the magnitude of hemodynamic response to dobutamine during DSE.

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Abbreviations

DSE:

dobutamine stress echocardiography

HR:

heart rate

SBP:

systolic blood pressure

DBP:

diastolic blood pressure

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Acknowledgements

This study was supported in part by grants from the National Institutes of Health HL68834 (to JAJ) and an American Foundation for Pharmaceutical Education Clinical Pharmacy Post-Pharm.D. Fellowship in the Biomedical Research Sciences (to CLA and LHC). Complete genotype, heart rate and blood pressure data from this study are available at www.Pharmgkb.org.

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Authors and Affiliations

  1. Department of Pharmacy Practice, University of Florida College of Pharmacy, Gainesville, FL, USA

    C L Aquilante, H N Yarandi, L H Cavallari, T E Andrisin, S G Terra & J A Johnson

  2. Division of Cardiology, University of Florida College of Medicine, Gainesville, FL, USA

    J F Lewis & K K Hamilton

Authors
  1. C L Aquilante
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  2. H N Yarandi
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  3. L H Cavallari
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  4. T E Andrisin
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  5. S G Terra
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  6. J F Lewis
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  7. K K Hamilton
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  8. J A Johnson
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Corresponding author

Correspondence to J A Johnson.

Additional information

Presentation of material as an abstract: These data were presented in part as an abstract at the 2002 Annual Meeting of the American College of Clinical Pharmacy, Albuquerque, New Mexico. Pharmacotherapy 2002 22:240.

Duality of Interest

None declared.

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Aquilante, C., Yarandi, H., Cavallari, L. et al. β-Adrenergic receptor gene polymorphisms and hemodynamic response to dobutamine during dobutamine stress echocardiography. Pharmacogenomics J 8, 408–415 (2008). https://doi.org/10.1038/sj.tpj.6500490

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