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Genotyping of the PXR A11156C polymorphism with locked nucleic acid containing fluorogenic probes

The Pharmacogenomics Journal volume 5, pages 72–74 (2005)Cite this article

The introduction of molecular technologies that combine the polymerase chain reaction (PCR) with the use of fluorescent hybridization probes significantly reduced the risk of contamination, since no post PCR steps are involved. Although these techniques have been successfully used for the identification of a huge variety of single-nucleotide polymorphisms (SNPs),1, 2 there are target sequences in which the discriminating power of DNA probes is not sufficient to differentiate between the two alleles of a biallelic gene locus.

The nuclear pregnane X receptor, PXR (also known as SXR, PAR, PRR and NR1I2), is an important component of the body's adaptive defensive mechanism against toxic substances including drugs. Ligand-activated PXR is involved in the transcriptional activation of multiple drug resistance gene (MDR1) and cytochrome P4503A4 (CYP3A4). Both are main regulators in the uptake and transformation of many orally prescribed medicines. Hence, knowledge with regard to known PXR polymorphisms, which influence the expression of MDR1 and/or CYP3A4, is therefore relevant to understand the interindividual responses towards drugs.3, 4, 5

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Authors and Affiliations

  1. Department of Clinical Chemistry, University Hospital Maastricht, The Netherlands

    R A M Op den Buijsch, J E de Vries, P A H M Wijnen, M P van Dieijen-Visser & O Bekers

  2. Department of Biochemical and Clinical Genetics, University Hospital Maastricht, The Netherlands

    J E de Vries & W J G Loots

  3. TIB Molbiol, Berlin, Germany

    O Landt

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Op den Buijsch, R., de Vries, J., Loots, W. et al. Genotyping of the PXR A11156C polymorphism with locked nucleic acid containing fluorogenic probes. Pharmacogenomics J 5, 72–74 (2005). https://doi.org/10.1038/sj.tpj.6500299

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