Abstract
A polymerase chain reaction with sequence-specific primers (PCR-SSP) system using primers with mismatches at the 3′ ends was developed to determine polymorphisms in IL-10 promoter region. Three previously described biallelic polymorphisms in IL-10 were linked in a 12 reaction PCR-SSP system and the method used to provide genotype data on 233 UK and 166 Polish controls. There are eight possible polymorphic combinations in IL-10 promoter gene but only three were observed in both control groups. Population frequencies of IL-10 genotypes show, in contrast to HLA, that UK and Polish frequencies are remarkably similar.
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Koss, K., Fanning, G., Welsh, K. et al. Interleukin-10 gene promoter polymorphism in English and Polish healthy controls. Polymerase chain reaction haplotyping using 3′ mismatches in forward and reverse primers. Genes Immun 1, 321–324 (2000). https://doi.org/10.1038/sj.gene.6363669
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DOI: https://doi.org/10.1038/sj.gene.6363669
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