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No evidence for association to the G72/G30 locus in an independent sample of schizophrenia families

Molecular Psychiatry volume 10, pages 431–433 (2005)Cite this article

SIR – Mapping efforts to characterize the genetic basis of schizophrenia have indicated chromosome 13q32 as a potential susceptibility locus. In 2002, Chumakov et al reported an association (since replicated) for schizophrenia with single-nucleotide polymorphisms (SNPs) on chromosome 13q32 at the G72/G30 locus.1, 2, 3 Evidence for linkage at this locus in our sample (NPL=2.95, P=0.001 at D13s174 (Mulle et al, 20054)) encouraged us to investigate this association. We obtained 159 trios from the NIMH Genetics Initiative (66 trios) and the Western Psychiatric Institute and Clinic at University of Pittsburgh (93 trios), and genotyped 11 associated SNP markers as reported by Chumakov et al. Despite evidence for linkage to this region, we fail to find evidence for association at the G72/G30 locus.

Ascertainment of schizophrenia trios has been previously described, for both the NIMH sample5 and the Pittsburgh sample.6 We selected pedigrees for our study if the pedigree had a case and DNAs from both parents were available. We obtained 66 trios from the NIMH resource, and 93 trios from the Pittsburgh sample. A total of 11 SNPs were selected for genotyping based on evidence for statistically significant association with schizophrenia as published in Chumakov et al. SNPs were genotyped using Taqman genotyping technology (ABI), in a 384-well format with 10 ng of DNA per reaction. For data quality purposes, at least 15% of the sample was genotyped in duplicate for each SNP assayed. Out of >750 replicates, zero discrepancies existed (average rate of missing data=3%). The TDT statistic, which accounts for transmission of alleles from heterozygous parents, was computed according to Spielman and Ewens.7 Parental genotypes were not imputed.

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Authors and Affiliations

  1. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, MD, USA

    J G Mulle & A Chakravarti

  2. Western Psychiatric Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, USA

    K V Chowdari & V Nimgaonkar

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  1. J G Mulle
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  2. K V Chowdari
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  3. V Nimgaonkar
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  4. A Chakravarti
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Correspondence to A Chakravarti.

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Mulle, J., Chowdari, K., Nimgaonkar, V. et al. No evidence for association to the G72/G30 locus in an independent sample of schizophrenia families. Mol Psychiatry 10, 431–433 (2005). https://doi.org/10.1038/sj.mp.4001619

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