Abstract
In vivo the extravillous trophoblasts (EVTs) penetrate the decidua and the first third of the myometrium to remodel the uterine spiral arteries and achieve the high-flow, low-resistance circulation characteristic of the intervillous space of the term placenta. Much of our understanding of these processes comes from histologic analysis of placental bed biopsies, a limited tissue source and one that can provide only a snapshot of a dynamic process. To better characterize these cellular interactions, we have developed an in vitro co-culture system in which first trimester villous explants are cultured at low oxygen tension in contact with 2-mm2 sections of decidua parietalis from the same patient. Hematoxylin eosin counterstaining of paraffin sections shows that EVT columns form at the tips of the placental villi and adhere and penetrate the decidual surface. The decidual blood vessels in the path of the EVT show morphologic disruption. Immunohistochemical analysis of the co-cultures using both an endothelial specific anti-CD31 and an anti–smooth muscle actin antibody show a disruption of the integrity of the vessel lining together with a complete loss of organized smooth muscle actin surrounding the blood vessels. In contrast control decidua samples in the absence of placental villi exhibit blood vessels with a complete endothelial lining and an organized muscular sheath. Using both an anti–cytokeratin-7 and anti–Cdx-2 antibody specific to trophoblasts, we show that these changes coincide with invasion of the vessels by endovascular trophoblasts and penetration of the decidua by interstitial EVTs. No EVTs were found in the control decidua. Thus we conclude that this in vitro model mimics the physiologic change observed in vivo during trophoblast invasion into maternal decidual tissues, and as such it may provide useful information concerning the interactions between EVTs and decidual cells and vessels during early gestation.
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In normal human pregnancy, the blastocyst attaches to the uterine epithelium and implants into the endometrial stroma, giving rise to the extra-embryonic trophoblast shell, the precursor of the hemochorial placenta. Simultaneously the endometrial stroma transforms into the decidua under the influence of progesterone. Decidualization is characterized by the enlargement of endometrial stromal cells, the growth of the uterine spiral arteries, and infiltration of the stroma by macrophage and large granular lymphocytes or uterine natural killer cells (Benirschke and Kaufmann, 1995). In hemochorial placentation the trophoblasts differentiate along two main pathways: villous and extravillous. The villous trophoblast comprises the cytotrophoblast and the overlying syncytiotrophoblast of the villous tree, which is bathed by maternal blood in the intervillous space. Extravillous trophoblasts (EVTs) are derived from the tips of some anchoring villi where villous cytotrophoblast cells proliferate to form the cell columns from which individual EVTs separate, invade, and migrate into the maternal decidua. (Aplin, 1991; Loke and King, 1995).
The first trimester of trophoblast invasion and placental development occurs in a low-oxygen environment, as the blood flow to the intervillous space is not yet established (Genbacev et al, 1996; Rodesch et al, 1992). During this time the anchoring villi of the developing placenta give rise to two populations of EVT: the interstitial (InEVT) and the endovascular (EnEVT). The InEVTs invade as far as the superficial layer of the myometrium and appear to preferentially home toward the uterine spiral arteries and encircle them (Pijnenborg et al, 1980; 1983). The EnEVTs arise as groups of trophoblast cells, which detach from the trophoblastic shell and invade the lumen of the decidual uterine spiral arteries. The EnEVTs form loose plugs in the apical portions of these vessels, and communication with the intervillous space is restricted to a network of intercellular channels (Burton et al, 1999). Concomitant with trophoblast invasion, the walls of the maternal spiral arteries are destroyed. Fibrinoid necrosis of the vessel media and a loss of endothelium occurs, converting muscular vessels into flaccid sinusoidal sacs. (Boyd and Hamilton, 1970). This vascular transformation is necessary to accommodate the huge increase in blood flow (3.5-fold) to the intervillous space required to successfully support the developing fetus. (Thaler et al, 1990; Ziegler et al, 1999).
There is some controversy as to the roles of the two EVT populations in the transformation of the spiral arteries. Immunohistochemical studies of placental bed biopsies suggest that EnEVT and endothelial cells transiently coexist on the walls of partially modified spiral arteries. The EnEVT are thought to migrate along the vessel lumen and adopt a more endothelial cell phenotype as they replace the endothelium and most of the musculoelastic tissues of the vessel walls (Pijnenborg et al, 1980; Zhou et al, 1997b). However, a study examining the change in decidual spiral arteries found that medial necrosis and replacement with fibrinoid material only occurred in the presence of InEVTs, whereas it was the EnEVTs that were responsible for replacing the endothelial cells in the transformed arteries (Kam et al, 1999). These authors suggest that the InEVTs prime the vessels in some way for invasion by the EnEVTs (Kam et al, 1999). Failure of the EnEVTs to acquire an endothelial phenotype and to proceed beyond the superficial portions of the spiral arteries is thought to contribute to complications of pregnancy such as intrauterine growth retardation, pre-eclampsia, and spontaneous miscarriage (Lim et al, 1997; Lyall, 2002; Zhou et al, 1997a). Previous work has shown that the interstitial invasion is not affected in these cases (Pijnenborg et al, 1998), suggesting that the ability of the EnEVTs to enter and interact with the vascular cells of the spiral arteries may be the key difference between normal and pre-eclampsia and intrauterine growth retardation pregnancies (Khong et al, 1986). However, more recent studies have reported a decrease in the depth of interstitial invasion in pre-eclamptic pregnancies (Kadyrov et al, 2003), which in turn may impact the endovascular invasion. These conflicting reports highlight the need for further understanding of the mechanisms behind these critical cell-to-cell interactions in both normal and pathologic pregnancies.
Studies of trophoblasts and spiral artery interactions have been confined to immunohistochemical analysis of placental bed biopsies and pregnant hysterectomies (Kam et al, 1999; Lyall et al, 1999; 2000; Zhou et al, 1997a; 1997b), which are limited to a single time point. In vitro studies have also been limited by a lack of suitable models, although recently a study investigating the perfusion of isolated myometrial arteries with either primary trophoblast or EVT cell lines has shown that both interstitial and endovascular interactions could be detected (Cartwright et al, 2002). However, these authors used myometrial tissues from term pregnancies, which may not be relevant to the cellular interactions that occur during the first trimester within the decidua. In this study we present a novel in vitro model of decidual spiral artery remodeling using co-cultures of first trimester decidua parietalis and placental explants.
Results and Discussion
First trimester placental villi cultured in contact with the apical epithelial surface of the decidua adhere to the decidual fragments (Fig. 1A) and show the formation of EVT cell columns that penetrate the decidua in paraffin sections (Fig. 1B). Immunohistochemical analysis of these co-culture sections using a monoclonal anti–cytokeratin-7 (CK-7) antibody demonstrated strong immunoreactivity in the villous trophoblasts of placental villi and the EVT cell column (Fig. 1C). Moreover, both InEVTs and EnEVTs were detected within the decidual stroma and spiral arteries, respectively, extending from the trophoblast cell column into the decidua (Fig. 1C) over the culture period of 6 days. The EVTs appeared to preferentially enter the lumen of the arterial blood vessels where they formed loose cohesive structures in the proximal portions of the decidual spiral arteries, similar to the trophoblast plugs observed in vivo during early pregnancy (Fig. 1D). Beyond the trophoblastic plugs, the endovascular trophoblasts appeared to migrate down the endothelial surface, causing a disruption of the endothelial cell integrity as they moved and relined the vessel with EnEVT (Fig. 1, D and F). The EnEVTs also appeared to invade the vessel wall, enter the decidual stroma, and migrate toward other blood vessels becoming InEVTs (Fig. 1C). In this model the uterine veins are never invaded, showing no staining with CK-7 and an intact endothelium (Fig. 1, D and E; Fig. 2, A to D). In a total of 29 vessels (n = three to six per experiment) analyzed from six experiments, invasion by trophoblasts occurred in 69.86 ± 6.1% in the proximal regions of the decidua and extended to the distal portions in 53.2% ± 7.2% by 6 days of culture. Study of earlier time points revealed that EVT columns required 3 days to attach the placenta to the decidua; and by 4 days of culture, the proximal arteries displayed signs of invasion, which extended to the distal portions by the 6th day.
In the present study, CK-7 staining was also routinely detected in the epithelium of decidual glands of both invaded and noninvaded tissues (Fig. 1G). It should be noted that longitudinal sections of decidual glands distinctly resemble transformed spiral arteries displaying a layer of CK-7 positive cells, a lack of endothelium and small focal depositions of smooth muscle actin (SMA). Therefore, great care should be taken in the identification and analysis of decidual blood vessel remodeling when using CK-7 as a marker to identify invaded trophoblasts. In this study we have used a monoclonal antihuman caudal-related homeobox gene-2 (Cdx-2) antibody as a potentially more specific marker of trophoblasts in the placental-decidual co-cultures. Cdx-2 has previously been identified as an intestine-specific transcription factor where it is thought to regulate the proliferation and differentiation of intestinal epithelial cells during crypt formation (Silberg et al, 2000). Beck et al (1995) reported the early expression of Cdx-2 in the trophectoderm of Day 3.5 mouse embryos and continuing expression in the fetal membranes and spongiotrophoblasts at later stages, suggesting that this homeobox gene may also be involved in placental development. In this study the placental-decidual co-cultures displayed strong Cdx-2 immunostaining in the EVT cell column (Fig. 1J) and in EnEVT lining decidual spiral arteries (Fig. 1H). This is the first identification of Cdx-2 as a novel marker of human trophoblasts. In contrast, in both the control decidua parietalis and placental-decidual co-culture, no Cdx-2 immunostaining was detected in the glandular epithelium, decidual stroma, or endothelial cells (Fig. 1I). Comparison of the CK-7 and Cdx-2 immunostaining in adjacent serial sections has allowed us to identify and distinguish between true transformed blood vessels lined with EnEVTs (CK-7, Fig. 1F; Cdx-2, Fig. 1H) and epithelial glands (CK-7, Fig. 1G; Cdx-2, Fig. 1I). Interestingly, in mouse placental development, the spongiotrophoblasts can differentiate into giant cells, and in this way they are analogous to the cells of the cytotrophoblast cell columns that anchor the villi of the human placenta (Rossant and Cross, 2001). Therefore, we suggest that Cdx-2 may also be important in the differentiation of human EVTs. Interestingly Cdx-2 staining was not observed in all luminal EnEVTs (Fig. 2D) as compared with CK-7 (Fig. 2A). We hypothesize that this localization to a subset of EnEVT suggests a potential role for Cdx-2 as a transcription factor in the differentiation of these invasive trophoblasts as they reline the blood vessels and interact with endothelium. The Cdx-2 staining therefore may reflect the different differentiated state of the EnEVT in an ongoing process of blood vessel transformation. These results identify Cdx-2 as a novel marker specific to the invasive trophoblasts of the human placenta.
Importantly, in the control decidua parietalis, no CK-7 or Cdx-2 immunostaining was detected in association with decidual blood vessels (Fig. 2G), showing that the EVTs observed in the placental decidual co-cultures were a result of de novo invasion in the experiment. In our model the trophoblast cells were observed to traverse the entire section of the decidua, migrating a distance of 5 mm over 6 days in culture. These results are in contrast to the observation made by Vicovac et al (1995), where in their placental-decidual culture system, trophoblast invasion was limited to the superficial surface of the decidua. We suggest that this discrepancy may be accounted for by the fact that these authors cultured their first trimester explants at atmospheric oxygen tension, whereas our model system is cultured at 3% O2 and 5% CO2 (conditions equivalent to first trimester pregnancy). When our experiments were cultured at atmospheric oxygen tension, the placenta failed to firmly attach to the decidual surface and was easily disrupted by the fixation process (data not shown).
In the co-culture system, decidual spiral arteries were observed where the endothelium had been almost completely replaced by CK-7– and Cdx-2–positive EnEVTs (Fig. 2, A and D). To investigate the effect of this trophoblast infiltration on the decidual spiral arteries, we used both a monoclonal anti-CD31 (platelet endothelial cell adhesion molecule-1) and anti-SMA antibody in serial sections to identify endothelial and smooth muscle cells, respectively. In invaded spiral arteries, a loss of endothelium was observed with a few endothelial cells visible but disrupted and detached from the vessel wall. These vessels were lined by a mixed population of trophoblasts and isolated endothelial cells (Fig. 2B). Staining with the anti-SMA antibody revealed that in the arteries relined by trophoblasts, the muscle cells had been completely lost, and only flecks of SMA remained (Fig. 2C, arrowhead) underlying the remaining isolated endothelial cells. However, in the partially invaded spiral arteries, focal degradation of the smooth muscle was observed with a gradual loss of SMA staining from the periphery of the vessel and in areas where the luminal EnEVT plugs contacted the vessel wall (Fig. 2E). In contrast, in the control decidua parietalis, anti-CD31 detected a complete endothelial cell layer lining the spiral arterial blood vessels (Fig. 2H). Swelling of the endothelial cells, particularly in the smaller arteries, was apparent, as has been previously described in decidua parietalis (Kam et al, 1999). The veins also displayed an intact endothelial layer, but the cells showed no swelling appearing as a flat monolayer (Fig. 2H). In these tissues the anti-SMA antibody detected an intact ring of SMA-positive medial cells surrounding the smaller arteries, while the larger spiral arteries displayed many layers of prominent medial cells (Fig. 2I).
These results agree in part with former in vivo observations in which EnEVTs and endothelial cells are reported to transiently coexist on the walls of partially modified spiral arteries (Pijnenborg et al, 1980; Zhou et al, 1997b). However, in our study EnEVTs were often observed in decidual vessels that were not surrounded by InEVTs, in contrast with the in vivo reports by Kam et al (1999), in which EnEVTs were only observed in vessels surrounded by InEVTs. We suggest that this discrepancy may be explained by the artificial presentation of the placenta to the decidua in this model. In normal in vivo implantation, it is the trophectoderm and not the EVT that makes the initial contact with the uterine epithelium, and the invasive trophoblasts do not develop until the blastocyst is fully implanted into the uterine wall (Benirschke and Kaufmann, 1995). Therefore, we suggest that the later EVTs resulting from 7- to 9-week gestational placentae used in this study may not possess the full ability to attach to and penetrate the epithelium to give rise to InEVTs. However, in this model the EnEVTs efficiently enter the lumen of the decidual arteries, replacing the endothelium and degrading the smooth muscle, which suggests that the EnEVTs possess the ability to remodel decidual arteries independently with no requirement for interstitial priming. Recently there has also been some controversy surrounding the adoption of an endothelial phenotype by the EnEVTs as they contact and replace the endothelium. It has been reported that the EnEVTs mimic the adhesion phenotype of the endothelial cells that they replace and that EVTs in the cell columns, InEVTs, and EnEVTs express CD31 (Zhou et al, 1997b). In this study use of monoclonal antibodies specific to CK-7 and CD31 has shown that during invasion of the decidual blood vessels by the EnEVTs, there is no expression of CD31 in any of the trophoblast populations. The CD31 immunoreactivity observed localized only to maternal and fetal endothelial cells of intact vessels and to the remaining isolated endothelial cells in the invaded vessels. These observations are consistent with reports stating that there is no expression of CD31 in the EVTs of the placental bed in the first trimester of normal, pre-eclampsia, and intrauterine growth retardation pregnancies (Lyall et al, 2001; Pijnenborg et al, 1998).
The anti–human leukocyte antigen (HLA)-G has been reported to be a good marker of EVTs within the decidua (Hunt et al, 1991; Kovats et al, 1990). However, the HLA-G antibody used in the present study is specific to the soluble form of HLA-G (sHLA-G). Interestingly on the placental-decidual co-culture sections, this antibody detected antigen only in the distal part of the placental cell column (Fig. 2G) and not in the trophoblasts within the decidua that had stained positively for both CK-7 and Cdx-2 in serial sections (data not shown). This result agrees well with published work that reports strong expression of the sHLA-G at the fetal-maternal interface (Le Bouteiller et al, 1999), where it has been suggested to contribute to the elimination of potentially harmful maternal cytotoxic allogenic T cells during pregnancy (Le Bouteiller, 2000). This result suggests that the EVTs may stop secreting sHLA-G as they enter the decidua and that HLA-G expression may be restricted to the cell-associated membrane form found on InEVTs and EnEVTs in vivo (Proll et al, 1999).
In conclusion these results suggest that this model of first trimester trophoblast and decidual cell interactions is able to mirror the extensive changes that occur in vivo during the remodeling of the decidual spiral arteries during the formation of the utero-placental circulation. As such we suggest that this model provides an excellent opportunity for placentologists to investigate the mechanisms underlying the cellular interactions between fetal trophoblasts and maternal endothelium in both normal and pathologic pregnancies.
Materials and Methods
Tissue Collection
First trimester placentae and decidua parietalis were obtained at the time of elective terminations of pregnancy. Informed consent was obtained from each patient, and the University of Toronto review committee on the use of human subjects approved collections. Tissue from 6 to 9 weeks of gestation was dated according to the criteria of the Carnegie classification evaluating characteristics of embryonic/fetal parts. Placental tissue was washed in ice-cold PBS, and amnion and umbilical cord were dissected away. Decidua parietalis from the same patient was washed in ice-cold PBS and assessed for the integrity of the apical epithelial surface.
Human Placental-Decidual Co-Culture
Placental-decidual explant co-cultures were established from first trimester human tissues by a modification of the method of Vicovac et al (1995). Placental and decidual tissue of 6- to 9-weeks gestation was placed in ice-cold PBS and processed within 2 hours of collection. Small fragments of placental villi (15- to 20-mg wet weight) were dissected from the placenta, teased apart, and selected for the presence of trophoblast cell columns. Decidua parietalis was assessed for thickness and integrity of the sample, dissected to 2- to 3-mm2 cubes, and placed with the apical epithelial surface uppermost in Millicell-CM culture dish inserts (pore size 0.4 μm, Millipore Corporation, Bedford, Massachusetts) precoated with 0.2 ml undiluted phenol red-free matrigel substrate (Becton Dickinson, Bedford, Massachusetts). The matrigel was allowed to solidify prior to the placement of the corresponding placental villous explant in contact with the decidual epithelial surface. Explants were cultured in 10% FBS DMEM-Ham F-12 media (Life Technologies, Burlington, Ontario, Canada) supplemented with 100 μg/ml streptomycin, 100 U/ml penicillin, and 0.25 μg/ml ascorbic acid, pH 7.4 at 3% O2 and 5% CO2. Culture medium was changed every 48 hours. Placental-decidual explants were maintained in culture for up to 6 days. Attachment of the distal end of the villous tips to the decidua, their adherence to matrigel, and the appearance of EVTs breaking through from the tips were used as markers of viability and morphologic integrity as previously described by Genbacev et al (1993). Explants (from a single patient) were cultured in triplicate for each treatment point. Each experiment was repeated with at least six placentae and matched decidua. Decidua parietalis was also cultured alone as a control to assess that there was no trophoblast invasion prior to the establishment of the culture or degradation of blood vessels due to the culture conditions.
Immunohistochemistry
Placental-decidual co-cultures and decidual control tissues were fixed in the insert by immersion in cold 4% paraformaldehyde for 1 hour and prepared for paraffin embedding. Histology was assessed on every 10th serial section using the standard hematoxylin eosin counterstaining protocol. For immunohistochemistry, 5-μm sections were deparaffinized in xylene and rehydrated through a gradient series of ethanol in PBS. Endogenous peroxidase activity was blocked by incubation of the sections in 3% hydrogen peroxide in methanol for 40 minutes. Antigens were unmasked using microwave pretreatment in 10 mm citrate buffer pH 6.0, (sections were incubated for 10 minutes at high power in the DAKO pressure cooker; DAKO Diagnostics Canada Inc., Missisauga, Ontario, Canada) or 0.02% Triton X-100 (Sigma, St. Louis, Missouri) pretreatment for the Cdx-2 antibody. Immunostaining used the HRP–LSAB technique (LSAB kit; DAKO). (For antibodies used in the study, see Table 1.) Negative controls were carried out by omission of the primary antibody or incubation with nonimmune mouse IgG. Slides were developed in 0.075% (weight/volume) 3,3-diaminobenzidine in PBS containing 0.002% (volume/volume) H2O2 with or without nickel enhancement (Vector Laboratories, Inc., Burlingame, California). After counterstaining with hematoxylin or nuclear fast red (Sigma), slides were dehydrated in an ascending ethanol series, cleared in xylene, and mounted with Permount (Fisher Scientific, Fair Lawn, New Jersey). Photomicrographs were taken using a Sony PowerHAD 3CCD color video camera DXC-970MD (Sony of Canada Ltd., Willowdale, Ontario, Canada) and Northern Eclipse software.
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Acknowledgements
This work was supported by a grant from the NIH (HD42558–01) and by a fellowship from the Canadian Institutes of Health Research (to CD).
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Dunk, C., Petkovic, L., Baczyk, D. et al. A Novel In Vitro Model of Trophoblast-Mediated Decidual Blood Vessel Remodeling. Lab Invest 83, 1821–1828 (2003). https://doi.org/10.1097/01.LAB.0000101730.69754.5A
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DOI: https://doi.org/10.1097/01.LAB.0000101730.69754.5A
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