Abstract
THE AP-1 family of transcription factors, which includes the proto-oncogene products c-Jun and c-Fos1, controls the stimulation of cellular genes by growth factors and the expression of oncogenes, including src and ras2–8. Transcriptional activation by c-Jun is regulated by a cell-type-specific inhibitor that represses the activity of a transcriptional activation domain (Al) of c-Jun by operating through the adjacent negative regulatory region (δ) (refs 9–11). Here we show that cotransfection of the src or ras oncogene enhances the transcriptional activity of a GAL4: c-Jun hybrid that includes the δ-A1 region of c-Jun, suggesting that the DNA binding and dimerization domain of c-Jun is not required for stimulation by Src or Ras. Moreover, induction of c-Jun activity by Src and Ras occurs in cell lines containing the c-Jun inhibitor but not in a cell line lacking it. The region in c-Jun essential for the stimulatory action of these oncogenes maps to domain Al. These findings suggest the existence of signal-transduction pathways that result in an increase in transcriptional activity of c-Jun and AP-1 by disrupting the c-Jun:inhibitor interaction.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Curran, T. & Franza Jr. B. R. Cell 55, 395–397 (1988).
Lee, W., Mitchell, P. & Tjian, R. Cell 49, 741–752 (1987).
Angel, P. et al. Cell 49, 729–739 (1987).
Ryder, K., Lanahan, A., Perez-Albuerne, E. & Nathans, D. Proc. natn. Acad. Sci. U.S.A. 86, 1500–1503 (1989).
Lamph, W. W., Wamsley, P., Sasson-Corsi, P. & Verma, I. M. Nature 334, 629–631 (1988).
Ryseck, R.-P., Hirai, S. I., Yaniv, M. & Bravo, R. Nature 334, 535–537 (1988).
Wasylyk, C., Imler, J. L. & Wasylyk, B. EMBO J. 8, 2475–2483 (1988).
Quantin, B. & Breathnach, R. Nature 334, 538–539 (1988).
Baichwal, V. R. & Tjian, R. Cell 63, 815–825 (1990).
Bohmann, D. & Tjian, R. Cell 59, 709–717 (1989).
Bos, T. J. et al. Genes Dev. 4, 1677–1687 (1990).
DeClue, J. D. & Martin, G. S. J. Virol. 63, 542–554 (1989).
Cantley, L. C. et al. Cell 64, 281–302 (1991).
Moran, M. F. et al. Proc. natn. Acad. Sci. U.S.A. 87, 8622–8626 (1990).
Schutte, J., Minna, J. D. & Birrer, M. J. Proc. natn. Acad. Sci. U.S.A. 86, 2257–2261 (1989).
Angel, P., Hattori, K., Smeal, T. & Karin, M. Cell 55, 875–885 (1988).
Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baichwal, V., Park, A. & Tjian, R. v-Src and EJ Ras alleviate repression of c-Jun by a cell-specific inhibitor. Nature 352, 165–168 (1991). https://doi.org/10.1038/352165a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/352165a0
This article is cited by
-
Ataxin-2 Modulates the Levels of Grb2 and Src but Not Ras Signaling
Journal of Molecular Neuroscience (2013)
-
Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2
Molecular and Cellular Biochemistry (2007)
-
Jun, the oncoprotein
Oncogene (2001)
-
Phosphorylation of c-jun mediated by MAP kinases
Nature (1991)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
